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urokinase n.【生物化學】尿激酶,尿致活酶。

urolith

Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth . ba - dfe was purified from the supernatant of b . amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation , ion - exchange chromatography on cm - and deae - sepharose fast flow , hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50 . the purified enzyme displayed thermophilic , hydrophilic and strong fibrinolytic activity 通過硫酸銨分級沉淀、 cm - sepharosefastflow和deae - sepharosefastflow離子交換層析、 phenylsepharose6fastflow疏水層析和sephadexg - 50凝膠過濾等方法,從解淀粉芽孢桿菌dc - 4的發酵液中分離純化出電泳純的ba - dfe 。

The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase , to make the scfv - linker - uk32 chimeric gene . this gene was cloned into the transfer vector pbacpak9 , and cotransfected with bacpak6 bsu36i digest into sf 9 cells . the fusion protein was secreted into the medium . in the fifth day after the cotransfection , the supernatant of the medium showed 107 iu ml fibrinolytic activity , higher than 25 iu ml fibrinolytic activity of scfv - uk32 . elisa showed that the supernatant had the binding activity to activated platelet . wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain 為了提高重組導向溶栓分子scfv - uk32的溶纖活性,通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列,并克隆到轉移載體pbacpak9上,通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內,進行表達。表達產物分泌到上清中,共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ,比未引入連接肽的scfv - uk32的表達活性25 iu ml高。

By means of pcr , the signal peptide dna sequence was deleted . for study of the limited factors in expression of pro - urokinase , the pro - uk gene was divided into three fragments , and they were expressed in 最后用同樣的方法提高全長人尿激酶原cdna在大腸桿菌中的表達量,使其表達量達到全菌蛋白的5 % 。

A clinic research on intraspinal injection of urokinase and exchange of cerebrospinal fluid for treatment of subarachnoid hemorrhage 大劑量腦脊液等量置換及椎管內尿激酶注射救治蛛網膜下腔出血后遲發性腦血管痙攣的臨床研究

The comparison of the repass rate between homemade recombined streptokinase and urokinase in thrombolytic therapy of acute myocardial infarction 國產重組鏈激酶與尿激酶在急性心肌梗死溶栓中的血管再通率的比較

Effects of urokinase - type plasminogen activator receptor on sperm chemotactic response induced by urokinase - type plasminogen activator in mice 尿激酶受體在尿激酶誘導小鼠精子趨化運動中的作用

Clinical observation on combined application of urokinase and troxerutin on deep venous thrombosis in lower extremity 患肢輸注尿激酶局部溶栓合用曲克蘆丁治療下肢深靜脈血栓形成的臨床觀察

Clinical observation of intrapleural injection of urokinase for patients with encapsulated tuberculous pleural effusion 胸腔內注入尿激酶輔助治療結核性纖維化性胸腔積液的臨床觀察

Application of low molecular heparin in acute myocardial infarction patients after urokinase thrombolysis treatment 目的探討哮喘患者未遵醫囑用吸入糖皮質激素

Therapeutic efficacy of recombinant streptokinase and urokinase in the treatment of acute myocardial infarction 重組鏈激酶與尿激酶治療急性心肌梗死的療效

The treatment of tramatic primary intraventricular hemorrhage with ventricular drainage applying urokinase 尿激酶引流治療外傷性原發性腦室出血

Comparison of therapy with recombinant streptokinase and urokinase for acute myocardial infarction 尿激酶溶栓治療急性心肌梗死的對比研究

Comparison the effects of recombinant streptokinase and urokinase in acute myocardial infarction 尿激酶對急性心肌梗死溶栓的療效分析

Clinical observation on urokinase collision treating frequent transient ischemic attack 基底動脈系統短暫性腦缺血發作56例療效觀察

Establishment of embolic stroke model and study of the efficacy of intra - arterial urokinase 犬腦缺血模型的建立及動脈內溶栓的研究

Studies were made on the enhancement of expression of human pro - urokinase pro - uk cdna in Arg使該片段的表達量提高了10倍。

Full ventricular irrigation with urokinase in the treatment of ventricular hemorrhage 尿激酶全腦室系統沖洗治療腦室出血

Effects of urokinase on hemorheology of acute cerebral infarction cases 尿激酶對急性腦梗死患者血液流變學的影響

Thrombolytic therapy of urokinase on acute myocardial infarction 尿激酶溶栓治療急性心肌梗死的臨床分析