x

trypsin

uploads/trypsin.jpg

trypsin n.【生物化學】胰朊酶，胰蛋白酶。

n. 【生物化學】胰朊酶，胰蛋白酶。 “bovine trypsin“ 中文翻譯：   牛胰蛋白酶“bromelain-trypsin“ 中文翻譯：   菠蘿蛋白酶-胰蛋白酶“crystalline trypsin“ 中文翻譯：   胰蛋白酶,結晶胰蛋白酶“crystallized trypsin“ 中文翻譯：   結晶胰蛋白酶“macroglobulin-trypsin“ 中文翻譯：   巨球蛋白-胰蛋白酶“tpck-trypsin“ 中文翻譯：   tpck胰蛋白酶“trypsin crystallized“ 中文翻譯：   結晶胰蛋白酶“trypsin digestion“ 中文翻譯：   胰蛋白酶消化“trypsin hydrolysis“ 中文翻譯：   胰蛋白酶水解“trypsin ihhibitor“ 中文翻譯：   胰蛋白酶抑制因子“trypsin inhibitor“ 中文翻譯：   胰蛋白酶抑制劑; 胰蛋白酶抑制素〔存在于大豆等中; 胰蛋白酶抑制因子“trypsin inhibrtor“ 中文翻譯：   抑肽酶,抑胰肽酶,胰蛋白酶抑制劑“trypsin, trypsinase“ 中文翻譯：   胰蛋白酶“trypsin-chymotrypsin“ 中文翻譯：   糜胰蛋白酶“alpha- and beta-trypsin“ 中文翻譯：   α-和β-胰蛋白酶; 和-胰蛋白酶“alpha-trypsin inhibitor“ 中文翻譯：   α-胰蛋白酶抑制劑“alpha1 anti-trypsin“ 中文翻譯：   α1抗胰蛋白酶“alpha1 anti-trypsin isotypes“ 中文翻譯：   α1抗胰蛋白酶同型“anti-trypsin ab“ 中文翻譯：   人抗胰酶抗體“basic pancreatic trypsin inhibitor“ 中文翻譯：   堿性胰蛋白酶抑制劑“bovine pancreatic trypsin inhibitor“ 中文翻譯：   牛胰胰蛋白酶抑制劑“cationic trypsin-1“ 中文翻譯：   陽離子胰蛋白酶-1“congenital pancreatic trypsin deficiency“ 中文翻譯：   先天性胰蛋白酶缺乏“egg white trypsin inhibitor“ 中文翻譯：   蛋白胰蛋白酶抑制劑“trypsase; parenzyme; trypsin“ 中文翻譯：   胰蛋白酶“trypsin digest agar medium“ 中文翻譯：   胰酶消化瓊脂培養基

trypsinogen

The clsp gene is located approximately 3 kb and 90 kb centromeric to the clr and cls gene , respectively , and their genes form a short cluster in a region of about 115kb in 12pl3 . the cluster gene arrangement and the sequence similarities of the cub and trypsin - like serine protease domain of clsp with other complement component 1 subcomponents supported a common evolutionary history by consecutive gene dup 同樣利用pcr檢測了clspmrna在人正常組織中的分布，結果發現，人clspmrna廣泛表達于人正常組織中，如在胎盤、肝臟、腎臟、胰腺中表達量較高，在肺、脾、前列腺、卵巢、結腸、外周血組織有中度表達，而在心臟、骨骼肌、胸腺、翠

After 4 days of growth , the intact inner cell mass ( icm ) were separated with 0 . 05 % trypsin - edta and replated on feeder layer in dmem containing 15 % serum , 0 . 1mmol / l nonessential amino acids , 0 . 1mol / l 2 - mercaptoethanol , 2mmol / l glutamine , 100 units / ml of streptomycin and 100 units / ml of penicillin . after 4 - 6 days 5 es cell colonies were selected and expanded in which alkaline alkaline phosphatase was detected ( 2 )小鼠囊胚或內細胞的培養和es細胞的分離培養了156個不同品系的小鼠囊胚，經過3 ? 4天培養后，將增殖出來內細胞團用機械法結合胰酶- edta處理，離散后培養于mef飼養層上， 4 6天后有5例出現了es細胞樣集落。

Compared to control , retention times of digesta in whole alimentary tract of immunized animals inc reased by 20 hours ( to use cumlative excretion of 5 % marker as reference ) . immunoneutralization of ss significantly augmented activities of digestive enzymes ( proteolytic , trypsin , chymotrypsin , amylase ) in pancreas and the small intestine ( control and immunized animals were 1693 . 67unit / g , cp , 2728 . 33 unit / g , cp , 3055 . 50 unit / g , cp , 12 . 9x106 unit / g , cp ; 2 . 57x 102unit / g , cp , 1 . 20x103unit / g , cp , 1 . 12x 103unit / g , cp , 2 . 98x 107unit / g , cp ft 2451 . 33 unit / g , cp , 2904 . 17 unit / g , cp , 4279 . 33 unit / g , cp , 20 . 61 x 106 unit / g , cp ; 6 . 45 x 102unit / g , cp , 2 . 53 x 103unit / g , cp , 1 - 83 x 103unit / g , cp , 5 . 77 x 107unit / g , cp , respectively , p < 0 . 05 or p < 0 . 01 ) 12ng ml ， 0人su vg ，各指標比較均差異不顯著， p 0刀5人兔疫組動物的食糜消化道滯留時間明顯增加（以指示劑累計排出50為標準，兔疫組較對照組大約增加20小時） ，與此同時， ss免疫中和也提高了胰腺和消化道各種消化酶的比活力（對照組和免疫組胰腺，小腸食糜總蛋白酶，胰蛋白酶， ”糜蛋白酶和淀粉酶比活分別為1693石7unit g ， cp ， 2728

The quickly developing techniques of biological mass spectrometry ( bio - ms ) in recent years realized the high throughput identification of proteins by determining the accurate mass values of trypsin - digested peptides and the randomly selected peptide sequence tags , and have been successfully used in the studies of protein interactions and post - translational modification such as the phosphorylation 摘要近幾年快速發展起來的生物質譜技術，依靠（酶解后肽段）精確質量數測定和隨機肽序列標簽分析，實現了對蛋白質高通量的鑒定，并被成功地用于蛋白質相互作用和蛋白質磷酸化等翻譯后修飾研究。

The cowpea trypsin inhibitor ( cptt ) gene is testified as a broad spectrum insect - resistant gene at present and its application in insect - resistant botanic transgenic engineering only after s / gene . the cpti transgenic plant developed rapidly for it ' s broad spectrum insect - resistant character and the target insects are uneasy tolerance to it 豇豆胰蛋白酶抑制劑( cpti )基因是目前在植物抗蟲基因工程中應用僅次于bt基因的廣譜性抗蟲基因。鑒于它抗害蟲的廣譜性和靶標昆蟲不易對其產生耐受性的優點，轉cpti基因植物得到了迅速的發展。

We treat the porcine skin by 0 . 25 percent trypsin , 0 . 125 % trypsin , 2 . 5 u / ml dispase , hypertonic saline or hypertonic saline - trypsin / dispase . we find that after the skin has been incubated in 0 . 125 percent trypsin for 24h at 4 ? , the cells in the skin are all disintegrated . there are no significant differentiation between the acellular matrix treated by 0 . 125 , 0 . 25 perlent trypsin , 2 . 5 u / ml dispase and hypertonic saline - trypsin / dispase . but the cell ca n ' t be removed by using the hypertonic saline - sds 本研究通過對0 25胰酶不同脫細胞時間處理、不同濃度胰酶處理、 dispase脫細胞法、 im 、 zm高滲鹽水脫細胞法、高滲鹽水和胰酶或dispase混合脫細胞法的比較確認采用0 12盼胰酶， 4 ， 244 。

The he was strongly inhibited by sbti and apmsf , and very sensitive to pmsf , lbti , and tlck , while not sensitive to chymostatin , bestatin , leupeptin , tpck , pepstatin , nem and iam . all these results imply that brine shrimp he was most probably a trypsin - type serine protease . the he could be strongly inhibited by edta in a dose - dependent manner , and 50 mmol / l edta exhibited more than 56 . 5 % inhibition 對孵化酶純化樣品進行生化性質和酶性質分析發現，鹵蟲孵化酶的最適反應溫度約為40 ，最適ph為8 . 5左右；該孵化酶對p - apmsf 、 sbti極為敏感，對pmsf 、 lbti和tlck也非常敏感，但對chymostatin 、 leupeptin 、 pepstatin 、 bestatin 、 tpck 、 nem和iam不敏感，表明該酶極可能是一種屬于胰蛋白酶類型的絲氨酸蛋白酶。

The results showed that the feeder layers prepared by icr and kunming mice had no significant effect on adherence of embryos and proliferation of icms . the trypsin , which affected the isolation of es cells remarkedly , should be used with concentration of 0 . 125 % trypsin + 0 . 02 % edta for kmsmin 從實驗結果來看， icr小鼠和昆明小鼠兩種來源的飼養層細胞對胚胎貼壁、 icm增殖無影響，消化液作用影響明顯，以0 . 125 trypsin + 0 . 02 edta消化10 15min為宜。

3 ) dispased cold digested single cell is the best to obtain pured epithelial cell line ; it is appropriate to obtain pured fibroblast cell line that repeated attachment and the pured method to their different sensibility to trypsin 對于秦川牛皮膚上皮細胞與成纖維細胞的純化： dispase冷消化單細胞培養的效果最佳，可獲得純化的上皮細胞系，而根據二者對胰蛋白酶的敏感性不同的分離方法、反復貼壁法適宜于獲得純化的成纖維細胞系。

When the hameoly - mph were pretreated with activators such as sds , trypsin and zymosan , their po acti - vities increased significantly , reaching about twice of the untreated . this indicated that the po activities exist in the hameolymoh of penaeus chinensis and penaeus 結果表明，中國對蝦和南美白對蝦血清中都存在po ，主要以酚氧化酶原（ propo ）的形式存在，并且都可以被胰蛋白酶、 sds和酵母聚糖激活。

Sequence alignment shows that the fragment of pcr product showed some identities to the masp gene of xenopus laevis , branchiostoma belcheri , the trypsin gene in litopenaeus vannamei and the serine protease gene in aurelia aurita Pcr反應產物基因片段大小為630bp ，序列比較結果表明， pcr克隆產物與爪蟾、文昌魚的masp基因，蝦、水母等的胰蛋白酶基因和絲氨酸蛋白酶基因都有一定的同源性。

If the skin has been put into 4 percent glutaraldehyde for three minutes or more . we are no longer able to get rid of the cells in it . the a - gal in porcine skin as disapered in acellular matrix treated by trypsin , dispase and sds 實驗發現4戊二醛對基質交聯固定后，若交聯時間短，則與不交聯組無差異，時間稍長，則胰酶無法脫除細胞。

In the experiment , in case of the proliferative capability of eg cells , the composition of 0 . 25 % trypsin + 0 . 04edta was optimal . the growth behavior of icr murine eg cells from pgc was observed 傳代過程中，以0 . 25胰酶與0 . 04 edta聯合作用效果較好，對eg細胞的綜合損傷最小，離散傳代后eg細胞活力比較強， eg克隆出現最多，最高傳至6代。

2 ) hanks “ buffer of trypsin not being added edta could obtain better the growth potential and the subsequent attachment of harvested skin cells t then it being added edta have better digested potent 與添加edta相比，用只含胰蛋白酶的hanks '液處理貼壁細胞，所回收的傳代細胞存活能力較好；而添加edta的胰蛋白酶消化液消化能力較強。

The po is very sensitive to some trypsin - specific inhibitors such as sbti , p - apmsf and bestatin , but almost not affected by trypsin - specific inhibitor tlck at all , indicates that it is like a tyrosine - type protease 在對蝦中也已報道了一些免疫促進劑，能不同程度地提高對蝦的自身免疫力，但其具體作用機理目前還不清楚。

For trypsin - like serine protease domain , the presence of his ( h ) - asp ( d ) - ser ( s ) representing a serine protease catalytic triad were at position of 283 , 339 , 436 , respectively Clsp在正常人外周血單核細胞來源的dc中有較強的表達，在分別經anti一cd40 、 tnf一a 、 lps刺激后的dc中，其表達水平均有所上升。

During the course of the primary culture and passage of es cells , co - culture of mouse es cells and mef was the best . in this research , cell digestive juice with 0 . 125 % trypsin + 0 . 02 % edta was relative gentle to cells 消化液濃度以0 . 125胰酶+ 0 . 02 edta對es細胞的損傷力最小，傳代后es克隆出現率最高。

Trangenic animals are very useful for delineating the function of newly discovered genes as well as for producing useful proteins in large animals ( e . g . the production of alpha anti - trypsin in goat ' s milk ) 轉基因動物對于描述新發現基因的功能和在大動物體內產生有益蛋白質十分有用。

In this experiment , the method of trypsin digestion was improved , and that several kinds of mouse fibroblasts and porcine fibroblasts were successfully dissociated and cultured 實驗中改進了胰蛋白酶消化法，成功地分離和培養了多種鼠成纖維細胞和多種豬成纖維細胞。