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trophoblast n.【生物學】滋養層。adj.-ic

trophology

The egfr expression in human trophoblast cells of dififferent pregnancy phases were shown by immunohistochemical method ( abc ) and western blot , and the related quantity of egfrmrna was analyzed by rt - pcr method . 2 . the ultrastructure of the placental tissue was observed under electron microscope 應用免疫組織化學及westernblot方法對不同孕期滋養層細胞的egfr蛋白的表達進行分析,應用逆轉錄一聚合酣反應( rt一pcr )技術對egfxillrtria進行定量分析,探討egfr在滋養層細胞分化發育中的作用。

The immunohistochemical method and western blot were preformed to show the expression of egfr and rt - pcr method was applied to determine the expression of egfr mrna in placenta trophoblast of pregnancy with pih . 3 . egfr of jar was determined by immunohistochemical method and egfrmrna was analyzed by rt - pcr method 電子顯微鏡觀察正常妊娠及妊高征患者兩組胎盤滋養細胞的超微結構,應用免疫組織化學方法及westernblot方法測定妊高征胎盤滋養層細胞表皮生長因子受體( egfr )的表達,以及rt一nr技術對egfrmrna進行定量分析。

The co - expression of l l6 - hsd1 and gr in the saxne chorionic trophoblast suggests possible intrcrine achons ofglucoconicoid generated by l l6 - hsdl within the cells . 2 . ewe radiomctric conversion assay showed that trctrient of the cells with the synthetic glucocorticoid - - dexarnethasone ( l0 - ' m ) for 24h increased the conversion of conisone to cortisol , and thes increase was blocked by the gr antagonist ru486 雙標免疫組織化學染色結果顯示11p hsdi和gr共存于同一個滋養層細胞,提示在體內無活性的gc代謝產物?一17羥d脫氫皮質酮經11p hsdi還原活化后可以直接與同一個細胞內的gr結合,即以內在分泌( intracrine )形式發揮作用

L lp - hsdl ma of i . 5kb was detected in the rna extracted from cult ' ured chorionic trophoblast using northem blot hybridhation method . the expression of ll6 - hsdl wa was induced by treating the chorionic cells with dexamethasone ( l0 - ' , l0 ' m ) for 24h , and the induction was blocked by co - treatinent of the cells with ru486 . these results suggest a gr - mediated effect of glucocorticoid on the rnrna expression and reductase activity of l l 9 - hsd1 人h合成的肌?一加x門’ m )對培養的絨毛膜滋養層細胞的11p hsdi還原酶活性具有誘導作用,此誘導作用可以被gr阻斷劑ru486門0川)所阻斷; northern印跡雜交方法進一步證實, dex亦可誘導絨毛膜滋養層細胞11p叫inrna的表達,此誘導作用也可以被ru486門0叫川)所阻斷

It acts as a mediator molecule for adhesion between the surface of blastocyst and epithelial cell , and maybe involved in implantation regulation , mmp - 9 is highly expressed and secreted by trophoblast cells , and the antibody of mmp - 9 can inhibit the invasion of the trophoblast this indicates mmp - 9 is very important to the invasive embryo Mmp - 9 (基質金屬蛋白酶- 9 )在植入過程中滋養層細胞大量分泌和表達,而多克隆抗mmp - 9抗體可以阻斷這些滋養層細胞對基質的浸潤,這說明, mmp - 9對于胚胎的侵入至關重要。

Many regulatory factors involved in the process of proliferation and differentiation of trophoblast were examined . epidermal growth factor ( egf ) plays an important role in normal cellular growth and differentiation and acts on its placental target cells , i . e . the trophoblasts , via a specific receptor ( egfr ) which belongs to the tyrosine kinase receptor family 表皮生長因子( epidermalgrowthfactor , egf )是一種分布廣泛,具有多種生物學效應的細胞因子,尤其對細胞的增殖具有強烈的促進作用, egf是通過與表皮生長因子受體( epidermalgrowthfactorreceptor , egfr )結合發揮其生物學效應。

Human trophoblast cells express both egf and egfr , and that egf plays a major role in placental implantation , growth and differentiation . an alteration of egfr biological activity directly interferes with the differentiated function and proliferation of trophoblast and the fetoplacental development Egfr的表達異常伴隨著胎盤滋養層細胞的分化增殖以及功能的改變,本研究目的旨在直接闡述egfr在滋養層細胞分化發育中的作用,以及與妊高征、絨毛膜癌發病的關系。

Which may provide the molecular basis for the inberine regulation of ll p - hsdl expression by glucocorticoids . secondiy , the regulation of l l 9 - hsdl expression and redutase activity by glucocorticoids was investigated in the primny cultured human chorionic trophoblast 整個妊娠過程中胎盤11 - hsd活性以氧化酶為主,使母體gc在進入胎兒循環前大部分被轉化為無活性的17 -羥- 11 -脫氫皮質酮,保證胎兒正常發育所需的激素濃度。

A growing number of evidences that human placenta is able to produce bioactive substance . these substances play a role in the proliferation of trophoblast cell and induction secretion of placenta hormones 越來越多的研究表明胎盤可以產生大量的生物活性物質,這些生物活性物質通過自分泌和旁分泌途徑單獨或協同作用于胎盤滋養層,影響其激素的分泌及細胞的增殖。

However , whether glucocothcoids modulate llp - hsd1 expression through a prgnoter dependent mechanism has never been studied . in this study we bo exainined whether 1l9 - hsdl and gr are co - expressed in the sarne chorionic trophoblast 胎盤中兩型酶均存在, 11 - hsd1主要存在于胎膜的絨毛膜滋養層細胞,而11 - hsd2主要存在于胎盤的合體滋養層細胞。

Main resuits and conclusions are as follows : 1 . after 3 days in culture , some of the chorionic trophoblast clustered together and formed mulh - nuclei syncghotrophoblast cells 主要實驗結果和結論如下1免疫組織化學染色顯示, 11p叫和gr免疫活性樣物質存在于培養的絨毛膜滋養層細胞

The trophoblast which displays highly proliferative properties between normal and tumor cells plays an important role in the establishment and maintenance of pregnancy 滋養層細胞生長特性處于正常細胞和腫瘤細胞之間,具有高度增殖能力及對母體的侵蝕性,并受多種因子調控。

Conclusions : the study suggests that the proliferation and adhesion of trophoblast in placenta of patients with early abortion was declined 結論:研究提示早期自然流產患者胎盤中滋養細胞增殖、粘附能力下降。

5 . 5 - ht at certain concentration , can markedly induce the hcg secretion of cultured trophoblast cells . 6 在一定濃度范圍內, 5 - ht可以促進培養的滋養層細胞的增殖; 6

To study the expression of egfr and egfrmrna in human trophoblast cells of different pregnancy phases 測定不同孕期胎盤滋養層細胞egfr蛋白的表達和egfrmrna的表達。

5 - ht at certain concentration , can markedly stimulate proliferation of cultured trophoblast cells 在一定濃度范圍內, 5 - ht可以刺激胎盤絨毛滋養層細胞分泌hcg 。

Egfr mrna and protein levels in jar were significantly higher than those in normal trophoblast ( p < 0 . 01 ) 妊高征組egfllin wa的表達也明顯低于對照組呼功

The obvious pathological changes were found in ultrastructure of placental trophoblast of study group 妊高征組胎盤滋養層細胞超微結構有明顯的病理學改變。

Analysis of transforming growth factor 1 mrna in trophoblasts of pih placenta 在妊高征患者胎盤組織的定位及定量研究