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triton n.【希臘神話】半人半魚的海神;〔t-〕【動物;動物學】...

tritone

Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7 . 5 , 250mm nacl , 5mm edta , imm dtt , 0 . 1 % triton , 50mm sodium orthovanadate , looug / mg pmsf and tpck , 50ug / ml tlck , 1 ug / ml leupeptin pepstatin and aprotinin ) , frozing them below - 70 , and then thawing them at room temperature for 10 min 2 . westem印跡將處理組及對照組小鼠一細胞g2期受精卵各200個取出,轉移到ep - pendoff管中, 5000甲m離心4分鐘,棄去上清,加入ro川粉碎緩沖液,充分振蕩混勻,在液氮中經過2一3次的凍融循環,迫使卵細胞裂解,加人等量的樣品緩沖液沸水中煮5分鐘,用于westem印跡分析。

In this study a microbial system for biphenyl biodegradation is set up in order to investigate the effects of surfactants on biodegradation of hydrophobic organic compounds . four strains which can grow on biphenyl as the sole carbon and energy sources are selected out , in which alcaligenes eutrophus dj1 and pseudomonas sms02 are chosen as degradation strains . after studing the bioavailability of three nonionic surfactants ( op - 10 , tween - 80 , and triton x - 100 ) , they are added into the biodegradation system of biphenyl 為了考察表面活性劑對疏水性有機污染物生物降解的影響,本論文選擇聯苯作為模擬研究體系,篩選、馴化得到四株可利用聯苯作為碳源生長的菌株;考察了聯苯降解菌株對三種非離子表面活性劑op - 10 、 tween - 80 、 tritonx - 100的生物可利用性;用高效液相色譜法測定了非離子表面活性劑對聯苯的增溶曲線;較系統地研究了加入非離子表面活性劑對聯苯生物降解速率的影響。

The expression protein products as insoluble inclusion bodies accounted for 15 % and 30 % of whole bacterial protein . two methods , b - per reagent only and triton - urine with sonication lysis of cells , were both access to the relatively good washing effect 通過摸索iptg誘導濃度,誘導溫度,菌體收獲時間等條件,確定了eo基因能在pgex4t bl21 ( de3 ) codonplus和pmal - p2x bl21 ( de3 ) codonplus中高效表達,表達產量分別占菌體總蛋白的15和30 ,表達的重組蛋白主要為包涵體。

After centrifugation at 8000rpm for 15 minutes , the bacterial pellets was subjected to ultr asonication in the suspension solution ( 50mmol / l , ph8 . 0 tris - hci , 100mmol / l nac1 , 0 . 5mmol / l edta , 4mol / l urea , 0 . 2 % triton x - 100 , 0 . 2 % doc ) at 300w , working 3s , intervul 3s for 240 times . then , concentration at 8000rpm for 15 minutes at 4 : 3 ) p (正x一們’一1一nk4表達菌株blzi誘導持續時間的研究分別進行了1 、 2 、 3 、 4 、 5小時的誘導,發現在誘導2小時后表山西醫科大學2003屆碩士學位論文達即可達到最人。

After washing with reagent ( 50mmol / l ph8 . 0 tris - hcl , 100mmol / l nacl , 0 . 5mmol / l edta , 2mol / l carbamide , 0 . 2 % triton x - 100 , 0 . 2 % doc ) , ultrasound crushing ( 200 w , 150 times , 3 seconds per time , spacing 3 seconds ) and freezing - melting methods , we got hng fusion protein and m - insulin fusion protein with purity of above 80 % . 4 . radioimmunoassay result shows that the radioimmune activity of of m - insulin fusion protein is 0 . 5 unit per litre bacterial liquid 3 .表達產物初步純化仁gly ”一flumanin融合蛋白和人胰島素突變體融合蛋白包涵體的洗滌方案為: lj ]體沉淀溶于含zmol , / l尿索洗滌液,超聲( 200w 、巧o次、 3秒/次、間隔3秒) , 4 、 i000orpm離心15min后沉淀用洗滌液充分重懸,一2 ( )凍存過夜,次日融化后4 、 10000rpm離心15min ,取上清,即得到初步純化的融合蛋白,可去除土要的雜蛋白,融合蛋白溶解于上清液中,純度達到80 %以_ 1 : 。

On cytomembrane ; resolved the precipitation in buffer d ( contained triton x - 100 ) shattered by ultrasound , 4 vibrated for 60min , centrifuged at 45000g for 1 hour , took the supernatent to mesure protein concentration and then mesure the pkc activity 目前,在鉛的神經毒性研究過程中,鉛對pkc的作用引起人們極大關注。這是因為鉛比鈣對pkc有更高的親和力。現已知, ph卜可以取代caz ”激活蛋白激酶c 。

A data assimilation experiment is carried out through combination the model with temperature measurements from the 165 e moorings on the equator , which collected by tao / triton ( tropical atmosphere and ocean project ) array of bugs 并通過在赤道太平洋, 165 e ,熱帶大氣和海洋( tropicalatmosphereandoceanproject ) ,即tao triton錨系浮標陣列觀測點,選取一段時間觀測資料,進行kalman濾波試驗。

Trypsin , dispase or hypertonic saline is used in decelliztion . triton x - 100 and sodiumdodecylsulphate ( sds ) are effective detergent in removing cell debris , but it is unknom that which of these is more effective 在去除細胞碎片方面,文獻中介紹以用曲拉通( tritonx 100 )和十二烷基磺酸鈉( sodiumdodecylsulphate , sds )為多,對于這兩種洗滌劑的選擇也有不同看法。

The cell debris ca n ' t be removed by using 0 . 5 percent triton x - 100 or the solution which is mixed by 0 . 5 percent triton x - 100 and 0 . 5 percent sds at the ratio of 1 : 1 . 25 , 1 : 1 , 1 : 0 . 75 , 1 : 0 . 5 , 1 : 0 . 25 , 1 : 0 . 125 125混合液洗滌,按本實驗方法效果不佳,而0 5 sds振蕩洗滌3小時可較好地清除細胞碎片。但是,經這樣洗滌清除細胞碎片后基質的物理性能與洗滌前相比顯著降低。

A triton mage from the forest of amber kelp has arrived with his menagerie . he was attacked by sea trolls and he is now seeking refuge in your lands 一個來自琥珀藻森林的特里同法師和他的家族來到了你這里。他被海巨魔攻擊而來你的土地尋求避難。

Some of such “ foreigners ” were standing among the multitude , just liked a triton of the minnows , and with no intention to cover up their sense of superiority 有些這樣的“洋人”就站在大眾之間,如同鶴立雞群,毫不掩飾自己的優越感。

Study on the optimum synthetic process of nano - hydroxyapatite particles by triton x - 100 hexanol cyclohexaneane inverse microemulsion system 正己烷反相微乳液體系合成納米羥基磷灰石的工藝探討

Mercury , venus , earth , mars , jupiter s moon io , and neptune s moon triton all show evidence of volcanic activity 在地球以外,水星金星火星木?一及海?一上均有火山活動的痕跡。

Effects of body weight on dominance hierarchy and agonistic behaviors in male greater long - tailed hamsters tscheskia triton 雄性大倉鼠體重對其斗毆行為及社會等級的作用

Synthesis of nano - hydroxyapatite particles by triton x - 100 hexanol cyclohexane inverse microemulsion system 反相微乳液合成納米羥基磷灰石的新方法

Tritons , nereids , and nymphs will always have a meaning on a fountain 海神特里同斯,女神涅瑞伊得斯還有水澤仙女他們總是與水息息相關。

He was just too big a man for the position , in fact a triton among minnows 其實他擔任那一職務,才力綽綽有余,有如鶴立雞群。

A triton prince has come to you in search of the fabled orb of atlantis 一個特里同王子來你這里尋找傳說中的亞特蘭提斯之珠。

If triton can ' t keep me drier than this , he can suck my cock 如果海神不能把我身上弄干點兒那就見他的鬼去吧