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trehalose n.【化學】海藻糖。

treillage

The main research contents include the methods for trehalose determination , the breeding of trehalose high - producing strain , the optimization of medium and culture conditions , the production of extracellular trehalose and the purification of the intra - and extracellular trehalose 研究內容主要包括海藻糖測定方法的研究、高產海藻糖菌株的選育、培養基與培養條件的優化、胞外海藻糖的合成以及海藻糖的提取與精制等。

The methods of paper chromatography - mottle elution cnromometry and enzyme - dns colorimetric were defined . these methods were simple , cheap and accuate . the results showed that the positive correlation was obtained between trehalose concentration and acetic acid or osmotic tolerant 提出了“紙層析-分離洗脫法” 、 “酶- dns比色法”定量測定海藻糖的方法,該方法簡便、成本低、準確度高。

Here we used a method of hplc / elsd to analyse the content from leaf extract of the two transformants and ck . there were 2 . 126 mg / gfw and 2 . 556 mg / gfw trehalose in these two transformants , respectively , whilst no trehalose was detected in ck 通過hplc elsd法測得這兩株轉基因煙草海藻糖含量分別為2 . 126mg gfw和2 . 556mg gfw ,而對照則檢測不出海藻糖。

In our report , we selected trehalose to substitute sucrose in the cryoprotectant . the process of vitrification is under the same preferable condition . the survival - rate of the recovered cells was tested by fda - pi and cd34 + cell - count 凍融后的細胞以fda - pi雙熒光染色、流式細胞儀cd34 +計數等檢測手段,與同樣條件的60蔗糖保護劑處理的細胞相比較。

Keeping the ph of the culture broth at 6 . 0 , the initial glucose concentration of l % , the strain could produce 14 . 5g / l trehalose after reaction at 50 for 6 hours , the production of trehalose increased by 62 . 2 % than that of flask - shaking culture 0 ,溫度50c條件下,發酵6h ,可使海藻糖的合成總量達14 sg l ,比搖瓶反應的海藻糖合成量增加了62 2 。

In order to enhance the trehalose production , y7 was used for mutation with ems . through primary screening and compound screening , strain l61 with high - yielding trehalose and deep neutral trehalase activity was screened 實驗對出發菌株y7進行了ems誘變處理,通過初篩、復篩,從中選育出一株能高產海藻糖同時具有較低海藻糖分解酶活性的菌株l61 。

The factors affecting the trehalose production were studied in this paper . the placket - burman design method and respond surface analysis method were used to optimize the medium and the reaction conditions were optimized too 應用正交設計、 plackett - burman實驗設計和響應曲面分析法確定了最佳的培養基配方,并對反應條件進行了優化研究。

When a protein is desiccated in the presence of trehalose , water molecules bound to the protein structure by hydrogen bonding evaporate and the sites are then replaced by the sugar to maintain an intact confirmation ( 42 ? 45 ) 當蛋白在海藻糖存在下干燥,經氫鍵結合在蛋白結構上的水分子蒸發掉,留下的位點隨后被糖替代,確保了結構的完整。

By response surface experiment , it was found that the combination use of 0 . 54 % peg6000 , 0 . 62 % trehalose and 0 . 31 % dtt could effectively improve the pal activity by 92 . 9 % 響應面實驗發現,海藻糖、聚乙二醇6000 、二巰基蘇糖醇在實驗濃度范圍內交互作用很小, 0 . 62 %海藻糖、 0 . 54 %聚乙二醇6000和0 . 31 %巰基蘇糖醇的組合可提高酶活92 . 9 % 。

The effects of six effectors , polyethylene glycol ( peg ) 6000 , d - sorbitol , trehalose , dithiothreitol , glycerol and glutarie dialdehyd on pal activity in enzymatic reaction were investigated in this article 摘要實驗考察了聚乙二醇6000 、 d -山梨醇、海藻糖、二巰基蘇糖醇、甘油、戊二醛對酶反應中苯丙氨酸解氨酶活力的影響。

Keeping the ph at 6 . 0 , 3 % of the initial glucose concentration , 5 % of seed volume , 1 % of glucose concentration , heat shock at 50 ? for 6 hours resulted in 8 . 94g / l trehalose 研究還發現,選用5接種量, 3初糖濃度,反應過程中底物濃度1左右, ph6刀, 50 ’ c條件下震蕩反應6h ,海藻糖合成量可達不94g l 。

Because trehalose exhibit protective action against damage of high molecular substances , it achieve widespread application in a variety of fields including foods , pharmaceutics and biochemistry etc 由于它具有對生物大分子非特異性的保護作用,從而在食品、醫藥、生物化學等領域得到了廣泛的應用。

Trehalose is accumulated at the end of the reproductive stages and under extreme environmental conditions . because the trehalose is intracellular product , its productivity is limited 在酵母生長后期或在不良環境的條件下可積累大量海藻糖,但由于是胞內產物,積累量有限,大大影響了產量的提高。

It was found that replacing part of granulated sugar in the complex solution of granulated sugar and sticky rice starch with trehalose could be effectively stay the course of retrogradation 研究發現,在含有糯米粉、白砂糖的復合體系溶液中,用海藻糖替代部分白砂糖,能有效地延緩回生的進程。

At this point , continual drying induces a phase change of the sugar to a glass state so that the protein molecules are surrounded by trehalose crystals , preserving them from thermal energy ( 46 , 47 ) 這時候,連續干燥引起糖的相變,變成玻璃狀態,這就使得蛋白分子被海藻糖包圍,從而避免受熱。

Meanwhile , the inhibition of trehalose for the retrogradation could be more apparent during the frequent courses of freezing and thawing , according to the analysis of the preservation condition 同時,通過對保存條件的分析,發現海藻糖在頻繁的凍融過程中更能體現出抑制回生的效果。

Through the test , the strain of y7 , which had the highest content of trehalose and strong tolerance to sodium chloride and acetic acid , was defined as a parental strain 綜合耐性實驗和海藻糖積累實驗的結果,確定了海藻糖含量高,滲透壓和乙酸耐性最強的y7菌株為本研究出發菌株。

Trehalose , a non - deoxidize disaccharide , serves as a protectant of enzymes and membranes in many microorganism , algae and lower invertebrate animals against various stress 海藻糖是由兩個葡萄糖分子以、 1 1糖苷鍵結合的非還原性雙糖,對許多生物的抗逆耐受性起著重要作用。

In yeast , the synthesis of trehalose is catalyzed by trehalose - 6 - phosphate synthase / phosphatase complex , which is encoded by four genes : tpsl , tps2 , tps3 and tsl1 酵母細胞中的海藻糖由tps1 、 tps2 , tps3和tsl1四個基因負責合成,其編碼的多肽組成一個海藻糖合酶復合體。