x
uploads/transgene.jpg

transgene n.【生物化學】轉基因。

transgenic

The results indicate the tobacco lines transformed with d - i - 2kl have white flowers and those transformed with r - 2kl and d - l - 2kl - r - 2kl do n ' t have obvious changes , similar to that of the wild tobacco . so we can speculate that : first , d - i - 2kl can restrain the synthesis of the tobacco anthocyanin ; second , the regulatory mechanism of the anthocyanin synthesis may be conservative among higher flowering plants ; third , there are not obvious changes in the flowers of tobacco lines transformed with r - 2kl ; fourth , r - 2k1 protein can inhibit the effect of ci - i - 2k1 , with the cooperration of c1 - i - 2k1 ; fifth , it is a possible way to alter the color of the plant flower by transgene 由此我們可以得到啟示:第一, c1 - i - 2k1蛋白的確能抑制轉基因煙草花青素的合成;第二,在高等植物中,有些物種間花青素合成的調控途徑可能存在保守性;第三,單獨的r - 2k1蛋白對轉基因煙草的花青素合成無明顯作用;第四, r - 2k1蛋白可能需要在c1 - i - 2k1蛋白協同下才會起作用,并且這種作用能抑制c1 - i - 2k1蛋白對轉基因煙草花青素合成的抑制作用;第五,通過轉基因的方法來改變植物花色有著明朗的應用前景。

The molecular mechanism of plant stress response was analyzed in this paper , the progress of transgene research for stress resistance related to different stress response pathway was overviewed , and the multi - gene integration strategy was proposed to extend the scope of stress resistance and improve its intensity 摘要分析了植物逆境應答的分子機制,概述了不同應答機制下的植物轉基因研究進展,提出了采用基因聚合技術實現不同類杭逆基因的聚合以提高轉基因植株的抗性范圍和抗逆程度。

Chloroplast genetic engineering , a new technology that could overcome many problems associated with nuclear genetic engineering , is of growing interest for bioproduction of valuable materials . it has several advantages including the extraordinarily high level of transgene expression and environmental safety , the favorable environment for prokaryote gene expression , the absence of “ position effect “ and gene silencing 葉綠體基因工程是隨著植物遺傳轉化技術發展剛剛興起的生物技術,具有超量表達外源基因,為原核基因提供適宜表達環境,消除“位置效應”和基因沉默,環境安全性好等優點,較更適合用于植物生物反應器方面的研究。

The transgene of choice is then injected into a fertilized egg , which is obtained as follows ; a female mouse is injected with several hormones to cause it to produce numerous eggs ; the female is then mated with a male so that some of the eggs are fertilized ; the eggs are collected before they divide and are injected with the foreign genetic material 然后將這段基因注入受精卵,方法如下:對一只雌老鼠注射激素使其產生大量卵;讓一只雄老鼠與其交配使部分卵受精;將這些卵收集起來,在其卵裂前注入外來基因物質。

Deficiency of apoe may promote to produce and develop atherosclerotic lessions . the apoe gene - targeted mice will result in marked regression of both early and advanced atherosclerotic lesions by injected apoe recombinant protein , or by transfected adviral vector with apoe cdna to express human apoe transgene in liver , or by transplantation of bone marrow with normal rat apoe gene . this demonstrates that apoe gene and its expressing product can inhibit progression of atherogenesis . apoe3 has a more effective prevention from as than apoe2 and apoe4 Apoe的缺失可促進動脈粥樣硬化的發生發展,給apoe基因敲除鼠反復注射apoe重組蛋白、在肝組織中用腺病毒載體表達apoe蛋白、移植帶有正常apoe基因的小鼠骨髓,都能使apoe基因敲除鼠的動脈粥樣硬化得到回復,表明apoe基因及其表達產物對動脈粥樣硬化的發生具有抑制作用, apoe _ 3對動脈硬化的阻抑作用要比apoe _ 2和apoe _ 4都明顯。

Now the biological and molecular assays are the major methods to assay cpti transgenic plant . there is no better method on assaying the expression of the transgene . the reason would be that the outcome of expression by cpti is a little peptide and its molecular weight is very small . its immu - nogenicity would be insufficiency so that it can not induce strong immu - nological reaction and can not produce special antibody of high potency 目前對轉cpti基因植物的檢測主要集中在生物測定和分子檢測,尚無較好的基因表達產物的檢測方法,其主要原因在于cpti基因的表達產物是小分子多肽,分子量小,免疫原性不夠,直接免疫不足以產生較強的免疫反應,不能產生效價高的特異性抗體。

The major purpose of transgenic detection is to identify whether they are gmos or not , how many about the transgene ingredient and whether they are approved and permit to import by our government , etc . previously , some transgenic detectin methods had been reported , but the detection method is simplistic not the general screening and identifying method 轉基因檢測的主要目的是鑒定是否為轉基因、轉的何種成分、含量多少以及是否為批準進口的品種等。有關轉基因檢測的方法,國內外已有不少報道,但具有單一性,沒有通用的篩選、鑒定方法,有關整合位點的研究國外剛剛開始,國內未見報道。

Organic food is referred to the environmental protective safe food which don ' t use synthetic pesticide , fertilizer , hormone , food additive and technology of transgene including provisions , vegetables , fruits , milk product , poultry , water product and beverage etc . in the process of production and procession 有機食品是指在生產和加工過程中不使用人工合成的農藥、化肥、激素、食品添加劑等物質,不使用轉基因技術的環保安全食品,包括糧食、蔬菜、水果、奶制品、禽畜產品、水產品、飲料等。

3 . labeling policies and regulations for gm products in different countries and territories and transgene detection method in gm products up to december 2002 , more than 40 countries and territories have adopted a variety of labeling regulations for gm products 三、轉基因產品標識管理政策與標準化檢測技術受國際政治、經濟因素的影響,迄今已有包括歐盟國家在內的40余個國家oi和地區制訂了相關的法律、法規,要求對轉基因生物及其產品進行標識管理。

Due to we cann “ t calculate accuratelly the efect on the safty of human and environment produced by transgene with current science and technology , consequently , it becomes the major measures to limit the transgenic crops enter our market in large numbers by detection and identification 由于目前科學技術水平還不能精確地預測轉基因可能產生的對人類及環境的安全問題,因而,開展轉基因檢測、鑒定,成為各國限制轉基因作物大量涌入的主要措施。

The applications of ion beam technology on the wheat breedings1 were elucidated , the principles and methods of breedings through ion - beam - induced mutation and ion - beam - mediated transgene were summarized and the parameters involved in wheat breedings in detail ware described 摘要闡述了離子束生物技術在小麥育種領域應用情況,概括介紹了離子束誘變育種和離子束介導轉基因技術的原理及方法,對離子束小麥育種過程中涉及的主要技術參數作了詳細描述。

Transgene flow to hybrid rice and wild relatives rice is one of the most important staple foods in china and gm rice with insect ; disease and herbicide resistance have been extensively developed which are in the pipeline readily for commercial application 一、轉基因水稻中外源基因向相關物種的漂流水稻是我國第一大糧食作物。轉基因抗蟲、抗病及抗除草劑水稻的產業化蓄勢待發,轉基因水稻的環境安全性研究迫在眉睫。

It have been reported that the 210 kb yeast artificial chromosome ( yac ) including the human a - lactalbumin gene could direct the transgene to express in a position - independent manner , therefore is an excellent expression vector to produce transgenic animal bioreactors 根據報道含完整人-乳白蛋白基因的yac載體能指導外源基因的乳腺特異性高效表達,是生產動物乳腺生物反應器的理想表達載體。

The result worked out here will provide strong scientific foundation and effective research approach when it comes to the research of wood quality and it ' s forming mechanism , wood transgene and directive breeding 這一結果將為木材性質研究、木材性質形成機理、樹木優質種質資源培育、樹木轉基因工程、定向培育材質改良的樹木新品種提供強有力的科學依據及研究方法。

Transformation of human lactoferrin gene to carrot may elevate the transgenic carrot nutritional and functional value , if the transgene expresses correctly , since lactoferrin is a multifunctional protein 把人乳鐵蛋白基因轉入富含類胡蘿卜素和植物纖維的胡蘿卜,讓它合成具有生物活性的人乳鐵蛋白,相當于在營養豐富的胡蘿卜中添加新的營養保健因子。

These results indicated that the cloned osg6b “ had a constitutive characteristics but not anther specificity , and the transgene was segregated in a mendelian fashion in the tl generation 研究表明,所克隆的osg6b ’不具有花藥特異性,而具有組成型表達的特征。第二部分:從大腸桿菌e

Gus examinaton showed that the rate of transgene was higher . and pcr analysis , pcr - southem and southern analysis all showed that chs was intigrated into quamocliy pennata chromosome successfully 同時,還以dig標記的camv35s上的一段為探針進行southern點雜交和pcr - southern雜交。

Using the leaves of “ royal gala “ as the explants , both vectors were transformed via agrobacterium - mediated transgene system . some transgenic plants have been obtained now 目前己經獲得了2個株系的反義轉基因植株和3個株系的正義轉基因植株,轉化頻率為0

Chemically inducible systems that control expression of native gene or transgene in plant have potential applications in gene function determination and genetic engineering 調控基因表達的化學誘導系統在基因功能鑒定及植物生物工程方面具有廣泛的應用價值。