T - tmv 中文 - EN166

tmv TMV ＝tobacco mosaic virus 煙草...

tn
Both lines showed resistance to tomv - 0 , tomv - 2 , tomv - 1 , tmv - u , tmv - cg and susceptible to tomv - 2a . this result indicated that tm - 22 was functionally expression in receptor cell with distinct genetic background . virus specificity and gene express can function in distinct genus within a family 為了了解tm - 2 ~ 2基因的特性、功能及抗病機制，研究工作從三個方面進行，首先將tm - 2 ~ 2基因轉化同科異屬的煙草sr1 ，以確定tm - 2 ~ 2基因在模式植物的表達的可能性和功能的完整性。
Sds - page results showed that there was a clear target protein band in mut + recombinant supernatant after 48 hours of culturing , while a faint band only in muts recombinant after 72 hours . western - blotting result showed that there was no remarkable difference of yield between mut + and muts recombinants after 6 days induced . anti - virus activity tests revealed that culture supernatants of mut + and muts recombinants could inhibit tmv infection with high efficiency in the same concentration and there was no significant difference between them 結果表明，誘導培養48小時后， mut ~ +重組菌株表達產物在sds - page膠上顯現出清晰的目的蛋白帶，而mut ~ s重組菌株培養72小時才能顯示微弱的目的帶； western - blotting雜交信號強度表明，同樣培養6天的mut ~ +和mut ~ s重組菌株表達產物在表達量上沒有明顯差別。
The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1 , respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus , which had no analogical to human gene . mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell 第三章，根據一種常見的病毒煙草花葉病毒( tmv )的核酸序列設計了分子信標熒光探針，由于tmv的遺傳物質是rna ，分子信標又具有很高的特異性和靈敏度，因此感染了病毒粒子的植物葉片在經過簡單處理后，可用分子信標檢測葉片上。
The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3 , then the vector was transferred into pachia pastoris gs115 strain . the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation . sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku 將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然后導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l . cv hetao flesh , which was cloned into plasmid vector pmd - 18 - t . the clon of antisense orientation were selected , and it was inserted downstream of camv35s promoter and enhancer “ “ of tmv into the plant expression vector pbinyxw , antisence expression vector pbinya was constructed . at the base that pollination and fertilization of cucumis melo l . cv hetao was studied , using pollen tube pathway transformate cucumis melo l . cv hetao , 76 fruit had been obtained , moreover , hardness and content of sugar were analysed 本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然后將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子“ ”的下游，構建成反義表達載體pbinya 。并在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，并進行了硬度和含糖量的分析。
The native expressed product from e . coli bl21 ( de3 ) strain , however , showed weak activity against tmv . gp609 and zwemu were inserted into pemu - mcs - n , an expression vector for monocotyledon . and rhxjb was inserted into pkylx71 : 35s2 將dna一8001連接到pet一sa表達載體上，在大腸桿菌blzi ( de3 )菌株中實現天然表達，表達產物對tmv的抑制效果很差，其原因可能是c一末端延伸序列的存在抑制了抗tmv活性的發揮。
The resuh twed that mb designed in consisten the the complndny sequence of a sechon of the conservative regions of tmv - rna was suitable for the direet assay of tmv - rna . another contrl experiined had been pefformed to testify that our resuit was re1iable or not Of 2inm之間有線性響應，檢測下限達8pm ， t ：；與共價交聯的固定方法相比，此傳感器具有較長的壽命和較好的穩定性。
It showed excellent anti - tmv activity . and the n - terminal 19 amino acid residues are “ dinfslagadgqtyn tfia “ ( accession number p83206 in swiss - prot ) 測得其n -端19個氨基酸序列： dinfslagadgqtyntfia ，與其它植物rip的同源性為10 73 ，與葫蘆科rip的同源性為37 73 。
A novel rip named gynostemmin ( 27 kda ) was purified from the leaves and stems of gpentaphyllum 它具有很高的抗tmv活性。其分子量約為27kda 。
Studies on virus resistance of transgenic tobacco with tmv replicase gene 轉煙草花葉病毒復制酶基因煙草的病毒抗性研究