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teleost n.【動物;動物學】新鰭類 (Neopterygii) ...

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When using the nuclear acid sequence and the predicted amino acid sequence of scghr to blast the fugu genome , we found that there were two contigs which shared the most similarity with scghr . therefore we realized there maybe were two ghrs encoded by different genes in teleosts . then , by means of nuclear acid sequence joining , orf of fghr1 and fghr2 were predicted from fugu genomic sequences using cohoghr isf1 , cohoghr isf2 , chghr and other related ghr amino acid sequence available at ncbi database 當利用南方鲇ghr的核苷酸、氨基酸序列blast東方?的基因組時,發現有兩個contig與其氨基酸序列非常相似,因此意識到魚類可能存在兩種不同基因編碼的ghr 。隨后主要利用已經克隆得到的cohoghrisf1 、 cohoghrisf2 、 chghr以及其他魚類ghr核苷酸和氨基酸序列blast東方純的基因組,采用序列拼接的方法從東方?基因組中分離出兩種不同基因編碼的fghr1和fghr2 (本研究把和傳統ghr相似的叫做ghr1 ,把另外一種叫做ghr2 ) 。

The complete nucleotide sequence of the mitochondrial genome of f . limnocharis was detailedly compared with those of 5 other amphibians . the nucleotide sequences of 22 trna encoded by 6 amphibians mitochondrial genomes were combined and aligned to the homologous sequences of the 11 veterbrate taxa . using teleosts as outgroup , the phylogenetic analyses results show that mp , nj and ml trees all strongly support the monophyly of living amphibians with respect to other living tetrapods and favor a sister group relationship for caecilians and salamanders 我們在測定了澤蛙線粒體全基因組序列的基礎上,與已知其它的5種兩棲類進行詳細的比較分析,同時選擇了11種高等脊椎動物的線粒體全基因序列,以硬骨魚類做外群,用22個trna基因合并數據進行系統發生重建分析,結果表明mp 、 nj和ml樹都強力地支持現生兩棲類動物為單系群并且蠑螈類和蚓螈類為姐妹群關系(自引導值分別為92 、 99 、 100 ) 。

After a comparison of all the ghrs isolated from the present study and others available at ncbi database , we find that ghr2 only exist in teleosts , and there is no such a gene in other vertebrates 在得到以上ghr序列后,又分析了人、鼠、雞等其他動物的基因組,發現ghr2只存在于魚類,是魚類特有的現象。本研究對ghr1和ghr2做了詳細的比較分析。

Jaillon , o . , et al . “ genome duplication in the teleost fish tetraodon nigroviridis reveals the early vertebrate proto - karyotype . nature 431 , no . 7011 ( 2004 ) : 946 - 57 一種叫金娃娃的硬骨魚的基因組復制揭示了早期脊椎動物的主要的染色體組型。 《自然》 , 431卷,第7011期( 2004年) : 946 - 57頁。