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supernatant

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supernatant adj.浮在表層的。n.浮在表層的東西；【化學】上層清液...

adj. 浮在表層的。 n. 浮在表層的東西；【化學】上層清液。 “decant supernatant“ 中文翻譯：   慢慢地傾倒上清液“hirt supernatant“ 中文翻譯：   海特上清液“liquid supernatant“ 中文翻譯：   上清液“liquor supernatant“ 中文翻譯：   上層清液“postmitochondrial supernatant“ 中文翻譯：   線粒體后上清液“stromal supernatant“ 中文翻譯：   基質上清液“supernatant antigen“ 中文翻譯：   上清抗原“supernatant factor“ 中文翻譯：   上清液因子“supernatant fluid“ 中文翻譯：   浮面液; 上層清液; 上清液“supernatant infection“ 中文翻譯：   上清感染法“supernatant layer“ 中文翻譯：   清液層“supernatant liquid“ 中文翻譯：   沉清液體; 澄清液體; 浮液; 浮于上層清液; 上層澄清液; 上層液體; 上清液“supernatant liquor“ 中文翻譯：   上層清液“supernatant tank“ 中文翻譯：   上清液槽“supernatant tuberculin“ 中文翻譯：   結核菌素上清液“supernatant water“ 中文翻譯：   表層清水；上層清水“supernatant(fluid)“ 中文翻譯：   上層清液; 上清液“breast carcinoma supernatant“ 中文翻譯：   乳癌上清液; 乳液上怪清液“clear supernatant liquid“ 中文翻譯：   上清液“normal lymphocyte supernatant“ 中文翻譯：   正常淋巴細胞上清液“platelet-free supernatant plasma“ 中文翻譯：   無血小板上清血漿“supernatant clarified liquor“ 中文翻譯：   上層澄清液“supernatant liquor pump“ 中文翻譯：   超汽蝕泵; 清泥液泵“supernannan“ 中文翻譯：   馬楠“supernal“ 中文翻譯：   adj. 崇高的，神圣的；〔詩〕天上的；超凡的。 supernal beings (住在天上的)神仙，天使。 supernal melodies 仙樂。 adv. -ly “supernaculum“ 中文翻譯：   n. (應該喝得一滴不剩的)美酒。 adv. 干杯；(喝得)一滴不剩。 drink supernaculum 喝光。

supernational

As concentration mostly existed in cytoplasmic supernatant when applying as in the medium . and the distribution percentage of as in cytoplasmic supernatant increased gradually from root to frond . the ca percentage in cytoplasmic supernatant followed the same trend as as 隨著砷處理濃度的提高，砷主要位于胞質中，從根部到地上部，砷在胞質中的分布比例逐漸升高，鈣在胞質中分布比例與砷相似，呈現從地下部到地上部逐漸增加的規律。

The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask . having been induced with methanol for 36 hours , the target enzyme could be examined in the supernatant by measuring amidolytic activity 首次將大連蛇島賅蛇毒類凝血酶成熟基回克隆到表達載體ppicgk中，經電激轉化至畢氏酵母菌株gs15中，再經甲醇誘導，在150ml搖瓶畔1獲得細胞外分泌表達產物。

An antifungal protein , named as b16 , was purified from the supernatant of the fermentation broth of strain 041381 by 80 % saturation ammonium sulfate , desalt and gel filtration on sephadex g - 75 , which with molecular weight at about 30 - 40 kd on the basis of sds - page 菌株041381發酵上清液經70飽和度硫酸銨沉淀，透析脫鹽， sephadexg - 75凝膠過濾層柱，得到活性物質b16 。以sds - page膠為基礎進行電泳分析， b16的分子量為30 - 40kd 。

Several kinds of factors associated with immunosuppressive functions by tumor cells had been detected in tumor culture supernatant , such as tgf - p , il - 10 , vegf . immunosuppressive cytokines affect lymphocyte responses , angiogenesis , and even tumor growth . most of them were reported to be soluble Exosomes是直徑在30 - 90nm之間的一類膜性囊泡，首先在細胞系的培養上清中被發現，并由網織紅細胞的培養上清中純化而得到命名。

Collect three groups culture supernatant for elisa experiment , rt - pcr and elisa results indicated that both transcription and translation of two cytokine genes can be enhanced obviously . rt - pcr results showed that the transcription level of il - 1 and tnf - a genes of oligochitosan contrast group were as 2 Rt pcr結果表明兩種細胞因子基因轉錄水平明顯提高，加糖對照組il一互p和tnf a基因轉錄水平分別為空白對照組的2

Medium experiments were arranged under uniform design , and then an optimum medium was got accordingly . the culture liquid was centrifugalized at 3 , 500r / min for 30min , then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others 通過硫酸銨分級沉淀、 deaesephadexa - 50陰離子交換凝膠層析和sephadexg - 75凝膠柱層析對發酵液進行分離和純化，并得到電泳純的酶。

5 $ methanol . sds - - page showed that after being induced with 0 . 5 % methano1 for 3d , the expressed products existed in supernatant and it ' s molecular weigh was about 22kd , western b1ot showed good antigenicity and speci ficity of expressed product 達產物經sds page檢測表明，轉化于表達了s ：丫hbsagm合蛋白，蛋白分子tie為22kd ， westernblot分析表明，表達蛋白具有較好的抗原性和特異性。

After inducement by methanol , the soluble part of trail was secreted into supernatant . a high - level strain named as gtr48 with more than 38 % of total protein secreted was screened . the foreign protein expressed amount to 10mg / l by estimate 我們篩選到一株高表達的菌株gtr48 ，經過甲醇誘導之后trail基因表達量占總的酵母分泌蛋白的38以上，并且初步估計其表達量可以達到每升十幾毫克以上。

Methods 1 mr1 hybridoma was cultured in hollow fibre system . culture supernatant was harvested , then precipitated by half - saturated ammonia sulphate . mfm was purified by deae - sephacel ion exchange chromatography . mri concentration was measured by elisa 硫酸錢半飽和沉淀， oeae陰離子交換層析分離純化抗co40l單克隆抗體mri 。 pbs透析后， 505一隊ge檢測純化抗體的純度， eusa測定抗體濃度。

The adhesive percent of ba decreased when the tested sugars , colostrums powder and the supernatants of bi , bl , salmonella and e . co / / . were added , while the adhesive percent of ba increased when the supernatants of ba and bbr were added 相同的實驗方法觀察到：所實驗的糖、初乳粉以及bi 、 bl 、大腸桿菌和沙門氏菌上清均能導致ba粘附下降，而ba和bbr上清卻有促進其粘附的作用。

The infected cells were passaged so that the virus could be isolated . cpe were observed from passage 2 to passage 9 postinfection . the cultured virus supernatants were harvested when cpe were observed in 3 / 4 infected cells 接種后的胚肺細胞從第二代到第九代均出現細胞病變，當接毒細胞有3 4出現細胞病變時，收集培養的細胞，制作超薄切片進行電鏡觀察，發現了病毒樣粒子。

The results of sds - page and western blot showed that the culture supernatant of transforments contained the correct glycosylated e2 protein and the recombinants could still secretedly express the specific proteins after culturing for 8 generations 經甲醇誘導表達后， sds - page和westernblot結果證明了p . pastoris培養上清液中含有正確糖基化的e2蛋白，表達量約250mg / l 。

Cells were centrifuged for 5min at 1000rpm and removed supernatant . the cell peller was resuspended in 1 ml culture media and divided into two glass flask . cell surface makers ( cd45 , cd90 ) were detected by immunocy - tochemistry 當傳到第五代時，取一瓶mscs用胰酶消化，以8x10vcm ‘濃度接種在六孔板中的蓋玻片上，應用免疫細胞化學方法檢測cd45人d90的表達。

Halocin c8 is quite stable , as it can be desalted , boiled , frozen , subjected to organic solvents , and stored in culture supernatant at 4c or in dh2o at - 20c for more than one year without losing activity Halc8性質非常穩定，可于100煮沸1小時而不失活；對去鹽作用不敏感；對蛋白酶k敏感，但對胰蛋白酶不敏感；對大多數有機溶劑如甲醇、乙醇、乙腈等不敏感。

Results : sds - page showed that after being induced with 0 . 5 % methanol foi 48 hours , the expressed product , a 20 kda glycoprotein that was secreted into the medium , existed in supernatant in the form of soluble molecule 5甲醇誘導后，目的基因己成功表達，而且目的基因表達量隨誘導時間延長而增加。經0 5甲醇誘導表達后，培養液上清中檢測到表達產物。

2 . the plasimd encoding human carcinoembryonic antigen was transfected with lipofectine into human dcs and human tumor cells such as mgc803 , ls174t and eel09 . the culture supernatants were collected for assaying carcinoembryonic antigen by . 3 1640培養液中，在37ac ， 5 co ，的培養箱內培養， ’每3心傳代一次，取對數生長期的細胞進行實驗。

Supernatants were collected and centrifuged ( at 4 ) sequentially once for 20 min at 1 , 200 x gmax , once for 30 min at 10 , 000 x gmax and once for 60 min at 100 , 000 x gmax 蛋白檢測發現來源于正常培養a20細胞的exosomes和來源于熱休克a20細胞的exosomes中均含有mhci類分子、 lamp和hsc7o等腫瘤細胞來源exosomes的特征蛋白。而且hs一ex 。

When the ph of acid liquid and supernatants was adjusted to 7 , there was no influence on growth trend of salmonella . but the amounts of salmonella in different growth stage were lower than the corresponding stage of the controlled group 將酸液和上清液的ph調至7后，對沙門氏菌的生長趨勢無影響，但各階段菌數均低于對照組。

The supernatant containing hsblys co - stimulated b cell proliferation highly with anti - igm by mtt analysis . flow cytometry results showed only in the function of costim lation , can b cell entered the s phase of cell cycle Mtt法知hsblys表達上清對b淋巴細胞具有共刺激作用；流式細胞儀分析顯示只有在共刺激作用下， b細胞才進入s期。