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subtil adj.(-tiler; -tilest) 〔古語〕=s...

subtile

In order to express alkaline protease gene ( ap gene ) in bacillus subtil is , the recombinant expression plasmid was constructed . this plasmid contains a promoter bp53 , also from b . pumilus un - 31 - c - 42 , ap gene and the shuttle vector psugv4 . after introduced into b . subtilis wb600 , the transformants displayed the hydrolyzed zone on milk plate 將來自短小芽抱桿菌un一31一c礴2的基因啟動子( bp53片段)和脫毛蛋白酶全基因( ap )進行融合,然后將重組基因(命名為bpap )插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中,構建成表達質粒psu一bpap 。

By transformation with the genes . plant disease biocontrol bacteria bacillus subtil is aplls and b . megaterium ap25 were isolated from wheat field soils collected from south australia and tai an . enzyme activity analysis on chitin agar and abp media showed that b . subtilis aplls secreted chitinase and b . megaterium ap25 secreted endoglucanase , respectively 測序后在genebank上進行序列比較,該基因片段同編號為2634966的枯草芽孢桿菌全序列的2599451到2812870 (功能未知)有85的同源性,但同已發表的13種幾丁質酶的基因(包括枯草芽孢桿菌幾丁質酶基因)的同源性很低,只有30 。

Sequencing analysis showed that the sequence of endoglucanase fragment exhibits 35 % homology with b . subtil is chromosomal dna ( from glyb to apre ) , and 27 % homology with bacillus sp 將此重組質粒phchi轉化巨大芽抱桿菌b . megateriumap25 ,得到兩株轉化子,分別命名為p25113一9 , p25113一10 。

But amnon had a friend , whose name was jonadab , the son of shimeah david ' s brother : and jonadab was a very subtil man 3暗嫩有一個朋友,名叫約拿達,是大衛長兄示米亞的兒子。這約拿達為人極其狡猾。

Pro 7 : 10 and , behold , there met him a woman with the attire of an harlot , and subtil of heart 箴7 : 10看哪,有一個婦人來迎接他,是妓女的打扮,有詭詐的心思。

Gen 3 : 1 now the serpent was more subtil than any beast of the field which the lord god had made 創3 : 1耶和華神所造的,惟有蛇比田野一切的活物更狡猾。

[ kjv ] and , behold , there met him a woman with the attire of an harlot , and subtil of heart 看哪,有一個婦人來迎接他,是妓女的打扮,有詭詐的心思。

And , behold , there met him a woman with the attire of an harlot , and subtil of heart 看哪,有一個婦人來迎接他,是妓女的打扮,有詭詐的心思。

Now the serpent was more subtil than any beast of the field which the lord god had made 耶和華神所造的,惟有蛇比田野一切的活物更狡猾。