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streptomyces n.(pl. streptomyces ) 【生物學】鏈...

streptomycin

The entire functional dnd gene cluster was minimized to an 8 . 3kb insert in phz1904 , because phz1904 could complement the dnd phenotype of s . lividans mutant strain zx1 as well as streptomyces parvulus atcc12434 and streptomyces nanchangensis ns3226 , two naturally dnd - deficient streptomyces strains 將這個8 . 3kb的區域以整合形式導入無dnd表型的兩個異源宿( ? )中,均使它們獲得了dnd表型,表明這段8 . 3kb的區域包含了完整的異常修飾的功能。

Base on the successful expression of pks gene by cits857 controlled lambda promoter , an e . coli expression vector phz330 , an e . coli and streptomyces bi - functional vector phz1060 were constructed containing the dts857 - pr for inducible expression in both hosts . both vector 根據受cits857調控的p _ r能引導pks基因的成功表達,構建了大腸桿菌表達載體phz330以及在大腸桿菌和鏈霉菌兩種宿主中都能表達外源基因的雙功能表達載體phz1060 。

Using these vectors , expression of the pks gene was achieved both in streptomyces lividans and in e . coli in a heat - dependent manner , suggesting that the lambda promoter and temperature - sensitive lambda represser functioned in s . lividans as well as in e . coli 利用這些質粒在變鉛青鏈霉菌和大腸桿菌中均表達出pks蛋白,兩種宿主中的表達都是熱依賴的。暗示噬菌體啟動子和溫敏型阻遏物在變鉛青鏈霉菌和大腸桿菌中都具有功能。

A novel dna modification discovered in streptomyces lividans is different from dna methylation . this unusual modification causes wild type s . lividans dna sensitive to site - specific oxidative double - strand cleavage ( dnd phenotype , dna degradation ) 變鉛青鏈霉菌的dna異常修飾系統是一種不同于甲基化修飾的新型修飾系統,它可使變鉛青鏈霉菌dna在電泳時易遭到氧化雙鏈切割( dnd表型, dnadegradation ) ( zhouetal . , 1988 ) 。

Fr - 008 and streptomyces griseus imru3570 are two independently isolated producers for a similar heptaene macrolide antibiotic . comparative studies between the two producers at chemical and biological level are the main task of the present work , no obvious difference was observed on the hplc profiles 本文主要從化學和生物學兩個角度分析和比較了這兩個菌株的異同,并對抗生素的氨基海藻糖殘基基因進行了克隆嘗試。

14 out of 25 strains of biocontrol agent streptomyces showed chitinolytic activity by biological assay . these 14 strains of biocontrol agent streptomyces were detected for chitinase gene using pcr primers derived from the conservative sequence of 17 cloned chitinase genes from streptomyces 此方法相對于幾丁質酶的傳統生物學檢測,具有更好的準確性和靈敏性;而且與生物學檢測相比,使用分子檢測省時省力。

An internal segment of the whig gene of s . griseus was amplified from plzl through pcr . the 304bp dna fragment was inserted into the ecori / bamhi site of e . coli - streptomyces shuttle plasmid pkc1139 , generating pkc1139 : : a whig , named plz107 , for gene disruption Pcr法克隆whig基因內部304bp片段,連接到大腸桿菌-鏈霉菌穿梭質粒嚇0139 ,構建了基因陽壞用重組質粒賊q139 : :凸mg ,命名plz107 。

Streptomyces are mycelial multicellular soil actinomycetes in which adverse environmental conditions induce a developmental program involving complex morphological differentiation coordinated with synthesis of an enormous variety of interesting and useful secondary metabolites 鏈霉菌是絲狀多細胞土壤放線菌,土壤逆環境誘導鏈霉菌具有復雜形態分化生命周期,獲得合成大量有用次生代謝物能力。

Fusobacteria are filamentous bacteria which secondary colonists on the dental plaque on your teeth , after it has been formed by species of streptomyces , thus thickening and reinforcing the bacterial attack on your teeth 梭桿菌綱(菌)是細絲狀的細菌在你的牙齒上的在牙齒的匾上的中級的殖民地居民,在它已經被鏈霉菌屬的種形成之后,如此使厚而且加強對你的牙齒的細菌攻擊。

Streptomyces hygroscopicus subsp . yingchengensis 10 - 22 is an important agricultural strain which produces at least four antifungal antibiotics named as antibiotic 5102 - i , 5102 - ii , 5102 - iii , 5102 - iv respectively 吸水鏈霉菌應城變種10 - 22是一種有用的農用抗生素產生菌,它可以產生至少四類抗真菌抗生素,分別命名為5102 ?號、 5102 ?號、 5102 ?號、 5102 ?號抗生素。

Integrated plasmids containing phage lambda promoter pr - directed and epitope - tagged 2 . 7 kb pks gene were constructed for tagging the natural fr - 008 pks with specific immunodeterminant ( epitope ) . these constructs were transferred into streptomyces sp 構建了帶有噬菌體啟動子和特異性抗原決定簇(表位)及2 . 7kbpks基因的整合型質粒,用于標記天然的fr - 008pks 。

70kb and 130kb in size respectively . though pks gene cluster for antibiotics fr - 008 was cloned , the genes for amino - mycosamine residue remained unknown . thus , attempt was also made to clone the desired gene ( s ) , streptomyces sp 首先從大量的發酵液中提取分離了鏈霉菌fr - 008產生的抗生素fr - 008 ,并通過大孔樹脂吸附、柱層析等手段進行了初步提純。

The chief aim of the present study is trying to extend the applications of yac and scp2 * , which have tremendous cloning capacity , for the above mentioned purpose . streptomyces sp . fr - 008 pks gene cluster ( c 本文對yac和scp2 *這類承載能力較大的載體系統在克隆fr - 008抗生素合成基因簇方面的利用進行了一系列載體改造和克隆策略方面的初步探索。

Many streptomyces species exhibit a very high degree of genetic instability which is usually manifested as genomic rearrangement such as large deletion , and high - level dna amplification of those sequences flanking the deletion 許多鏈霉菌表現出高度的遺傳不穩定性,通常為大片段缺失或基因組重排,以及缺失區域兩側序列的高水平dna擴增。

Strain sa - coo by southern hybridization . a cosmid - based gene library of streptomyces griseus atcc14811 was constructed using phz1357 , a streptomyces - e . coli bifunctional vector carrying two cohesive sites 為了獲得膽固醇氧化酶基因,以大腸桿菌-鏈霉菌雙功能柯斯質粒phz1357為載體,構建了灰色鏈霉菌atcc14811的基因組文庫。

Streptomyces whig gene is a key gene encoding a development ally important rna polymerase sigma factor ( awhig ) , which specifically initiates development program and determines the developmental fate of the cell Whig基因編碼一個發育上重要的rna聚合酶因子,特異性地起始發育程序,決定菌絲細胞的發育命運。

Later , researchers used fermentation to isolate large quantities of vitamin b12 in solution with bacteria streptomyces griseus , which technique is still widely used in the pharmaceutical field 后來,學者更進一步由發酵液中分離出維他命b12 ,而此種發酵液中分離的方法也是藥廠工業制造維他命12之主要方法。

The transformants were cultivated and the harvested cells were used in the procedure of conjugation by which the recombinant plasmid pid03 and pc05 were introduced into streptomyces avermitilis s - 2 respectively 經過在mym平板上的傳代和抗性標記的篩選分別得到了同源雙交換的菌株aved24和avecg 。

Anthonyl , pometto , crawforddl . effectsofphon lignin and cellulose degradation by streptomyces viridos2 porus [ j ] . appl . environ . microbiol . , 1986 , 52 ( 2 ) : 2462250 羅宇煊,張甲耀,許威亞,等.正交試驗選擇嗜堿細菌降解木質素的金屬離子最有綜合養條件[ j ] .環境科學與技術, 2001 , 4 : 527