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ribonuclease n.【生物化學】核糖核酸酶。

ribonucleic acid

Ribonuclease inhibitor ( ri ) is an acid cytoplasmic protein with the molecular weight of 50 kd . this ubiquitously distributed glycoprotein tightly binds and inhibits ang with a stoichiometry of 1 : 1 . the remarkable affinity of ri and ang supplies an approach to invalid the function of ang which is to stimulate neovascularization 核糖核酸酶抑制因子( ribonucleaseinhibitor , ri )能夠以1 : 1的比例結合并抑制ang ,這無疑為我們提供了一條重要的糾正ang引起的新生血管生長失控的途徑,從而治療相關疾病。

We designed a suitable probe in the experiment and used ribonuclease protection assay technology to detect the expression of pdipl gene in mouse liver at different time points after partial hepatectomy . we found that the expression of pdipl gene was increased . it reached the highest level after 12 hours and then decreased 我們設計合適的探針,采用核酸酶保護反應等技術測定了pdip1 -基因在肝臟部分切除后不同時間在小鼠肝臟中的表達,發現pdip1 -基因在肝臟部分切除后表達增強, 12小時表達最強,以后表達減弱。

This modification includes : ( 1 ) selecting two important molecules as candidates , ( 2 ) choosing a promiscuous t - cell epitope , and two b - cell epitopes or conserved amino acid sequences from the two important molecules , ( 3 ) connecting them adequately through analysis by the molecule designing software . therefore , the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing . based on the molecule designing methods , the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse , as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected 本研究以蛋白質分子設計的理論和方法研究避孕疫苗,將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合,獲得新抗原- 35肽序列;并在合成、純化后分別與弗氏佐劑、免疫刺激復合物( iscoms )混合后免疫不同遺傳背景的雌性小鼠,觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變:以及在ivf下,新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。

Angiogenin ( ang ) is a small monomer which universally exists in tissues and cells . angiogenin belonging to ribonuclease superfamily has 35 % homologous sequences with ribonuclease a ( rnase a ) 血管生長因子( angiogenin , ang )是一個小的單聚蛋白,與rnasea是同系物,在組織和細胞中分布廣泛。

Compared with ribonuclease a , the ribonuclease activity of ang is very low , but is very important to promote new endothelia proliferation Ang的rnase活性很低,但它裂解rna的活性對于它的血管生成活性非常重要。