x
uploads/recombinant dna.jpg

recombinant dna 【生物化學】重組脫氧核糖核酸。

recombine

The chinese university of hong kong has entered into a research agreement with genius international holdings limited to develop a technique to produce bone morphogenetic proteins ( bmps ) as a therapeutic device for bone fractures and defects . the new technique will involve the utilization of recombinant dna and protein engineering technology 香港中文大學和雅智國際集團有限公司( geniusinternationalholdingslimited )已達成合作協議,應用基因工程、蛋白工程及生化科學等多學科技術,開發及生產具有成骨生物活性的重組骨蛋白- 2 ( bonemorphogeneticprotein - 2 , bmp - 2 ) ,以應用于治療骨折及骨損傷。

By recombinant dna techniques , the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb . the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e . coli dh5a and induced with iptg . sds - page analysis showed an induced expression product band about 72ku , which correspond to the sizes of vp2 , reported in the literature 利用dna重組技術,將其結構蛋白vp2基因亞克隆至原核表達載體pproex - htb , iptg誘導后成功表達出與預期大小相符的約72ku的融合蛋白,光密度掃描對表達產物進行初步定量,表明表達產物約占菌體總蛋白的14 。

In order to express the recombinant peptide of both gp52 and pp150 oterminal peptides from human cytomegalovirus ( hcmv ) , which seem to show good antigenicity . recombinant dna technology was used to construct recombinant plasmid , which was transformed into the pichia pastoris to express the interesting peptide 為了表達人巨細胞病毒( humancytomegalovirus , hcmv )中抗原性較強的兩段蛋白片段? gp52c末端和pp150c末端的嵌合肽,用基因工程技術構建適于酵母表達系統的重組表達質粒。

Sensitive assays require a highly purified enzyme with a high turnover number , and a low detection limit for the reaction product . diagnostic enzymes produced by recombinant dna technology , which increased yield and quality of enzyme and simplified preparing process , improved the development of eia greatly 利用基因工程生產診斷用酶,使酶的純度大大提高,產量及質量均較自然來源酶好,簡化了制備程序,大大促進了酶免疫分析技術的發展。

Symposium participants reaffirmed that agricultural biotechnology will lessen the demand of agricultural products worldwide while protecting the environment and natural resources . the public concerns on recombinant dna technology and genetically modified food were also discussed 在研討會上,講者均重申農業生物技術對舒緩全球農產品需求及保護環境與天然資源的重要性,并討論了社會上對基因重組技術及基因食品的關注。

The proponents of recombinant dna research have decided to encourage federal regulation of their work ; they hope that by making this compromise they can forestall proposed state and local controls that might be even stiffer 重組dna研究的支持者已決定支持聯邦對他們工作的管理;他們希望通過這樣的讓步可以預先阻止州和本地可能更強硬的控制。

Then the recombinant dna was packaged in vitro . . identification of cdna library was followed , such as clone efficiency , recombinant efficiency , library integrality and the size of inserts 第二部分:以gt11為載體構建乳腺癌細胞t47d的cdna表達文庫;對構建的文庫進行克隆效率、重組效率、完整性以及插入片段的鑒定;并進行文庫擴增。

Co - signed scientists believe that recombinant dna technology can be applied to improve agriculture , healthcare and the environment , especially for the developing countries 宣言確認基因重組技術可應用于改進農業、健康及環境,對發展中國家所面對的問題有一定的幫助。

Recombinant dna technology is very important in genetic engineering . in this paper , we have constructed two recombinant vectors by it 重組dna技術是基因工程中的一項十分重要的技術,本研究即利用這一技術,構建出了兩個重組載體。

( when the host cell divides , copies of the recombinant dna molecule are passed to the progeny and further vector replication takes place 當宿主細胞分裂時,重組dna分子的拷貝隨之進入下一代細胞,同時也產生載體效應。

Through primary pcr . and finally the recombinant dna fragments were cloned into the phagemid pcantab 5e vector and introduced into 十五肽的接頭連在一起,并克隆到噬菌質粒pcantab 5e中,電擊轉化大腸桿菌tg1 。

They could also use recombinant dna to study cell differentiation and development , including such cellular abnormalities as cancer 他們還可能利用重組合dna來研究細胞分化和發育,包括像癌癥這樣的組胞變態。

We need a comprehensive assessment of the recombinant dna issue , preferably under the auspices of the federal government 對于重組體dna問題,我們需要有一個全面的估價,而且最好由聯邦政府主辦。

In the work , we got mutated plasmids pgex - 6p - m - centrein by means of pcr and recombinant dna techniques 隨著生物技術的逐步成熟,定點突變成為了科研人員進行科學研究的有力手段。

We presented the history of recombinant dna research and our guidelines , and invited the committee ' s opinions 我們介紹了重組體dna的歷史和我們的準則,并征求這個委員會的意見。

Preliminary study on immunotherapy of an oral recombinant dna vaccine of helicobacter pylori neutrophil activating protein 幽門螺桿菌中性粒細胞激活蛋白核酸疫苗實驗研究

Some success in interferon production by bacteria has recently been achieved by the recombinant dna technique 近年曾經應用重組dna技術使細菌產生干擾素獲得了一定成效。