Q - quinic 中文 - EN166

quinic adj.quinic acid【藥學】金雞納酸；【化學】...

quinic acid
Quinic acid , used shikimate pathway in e . coli , it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell . double specific enzyme genes arog , qutb or three ones arog , qutb , arob were co - expressed in a single plasmid pbv220 to improve the enzymes “ rate - limiting reactions . modifications of e . coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch 利用大腸桿菌莽草酸途徑合成新的代謝物奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向奎尼酸生成的方向。
5 . exploring fermentation synthesis of quinic acid ( qa ) using bioengineering strain under shake flask conditions synthesis of qa using recombinant e . coli constructs was examined in m9 accumulation medium . the result indicated that qa was produced from d - glucose in the culture supernatant , but it is necessary to explore further the consequences of fermentation in next work 工程菌搖瓶發酵產酸初步觀察初步探索了奎尼酸工程菌在m9積累培養基中的發酵產酸條件，結果表明工程菌經直接發酵由葡萄糖產生了奎尼酸，但尚須進一步優化發酵條件提高產酸率。
4 . engineering dhqase ( arod ) - deficient e . coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e . coli chromosome . the mutant 31bk was engineered , in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e . coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system . the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene , so it improved carbon flow into the quinic acid biosynthesis direction 構建宿主菌基因精確定位突變株31bk ( arod : : arobkan ~ r )為了改變代謝途徑脫氫奎尼酸( dhq )分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶( dhqase )失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，并減輕代謝負擔。
Quinic acid is one of aromatic small compounds , it has widely been used as the important raw material in the fields of medicine , food and chemical industry 奎尼酸是重要的芳香族小分子化合物之一，在醫藥、食品、化工領域有著廣泛的應用。
Synthesis with carbocyclic compounds , such cyclodienylsilane , quinic acid and norbornene , etc , as starting materials is discussed in detail 重點介紹了以環二烯基硅烷、奎寧酸、降冰片烯等碳環化合物為原料合成碳環糖的進展。
Metabolic engineering was introduced in the studies of recombinant e . coli constructs to produce quinic acid 我們將代謝工程技術應用于產奎尼酸基因工程菌的構建。