P - ptg 中文 - EN166

ptg Ptg. ＝Portugal; Portuguese.

ptisan
Its content was about 9 . 8 % among total cell protein by gene genius bio imaging system . the fusion proteins were found largely in an insoluble inclusion bodies . the purified fusion proteins was obtained by his6 technique used to immunize rabbits to obtain polyclonal antiserum with titer of 1 * 105 經工ptg誘導，重組質粒在點co力‘ blzi中表達出了c端融合了6xhis的融合蛋白，過量表達的蛋白主要以不溶性蛋白形式存在，其表達量占菌體總蛋白的9 . 8 % 。
Purified fusion protein gst - hnadc3 was used as an immunogen to inoculate rabbits and the antibody against the gst - hnadc3 fusion protein was raised , and was purified by gst sepharose 4b affinity chromatography to remove the antibody against gst Ptg誘導表達，超聲破碎細胞后，采用親和層析方法純化融合蛋白gst十nadc3 ，并以此為抗原免疫新西蘭株白兔制備融合蛋白抗體。應用親和層析的方法對gst十nadc3融合蛋白抗體進行純化，以去除抗gst抗體。
The e . coli strain jm109 was transformed with resultant plasmid pgex - 4t - 1 / 6 - 4 . 4 . the transformation was induced with iptg , then the total protein from cell extract was analyzed by electrophoresis on a 8 % sds - page in order to validate the gst fusion protein , and the fusion protein is about 90kd 4 .用工ptg誘導含pgex一4t一1 / 6一4的轉化菌，提取初提物中的總蛋白，進行sds一聚丙烯酞胺凝膠電泳，檢測表達的融合蛋白大小越為gokd 。
5 . after induced with iptg , jm109 with pgex - 4t - l / 6 - 4 was radiated by uv for 30 seconds , cultivate these e . coli with light or without light . the survival rate proved the gene of d . salina ( 6 - 4 ) photolyase has photoreactivation 5 .工ptg誘導過的轉化菌，經過30秒的紫外光照射后，分別進行光培養或暗培養，計算存活率，從而證明了及sa了z ’ na的( 6一4 )光裂合酶在大腸桿菌中可進行光修復活性作用。