P - proventriculus 中文 - EN166

proventriculus n.(pl. -li ) 【動物；動物學】1.鳥的前胃。...

prover
Ghrelin regulates pituitary growth hormone ( gh ) secretion . in the study , we obtained the cdna sequences of preproghrelin and the cdna encoding the ghrelin of duck , goose and emu by using rt - pcr and 3 “ - race methods . and then deduced the amino acid sequences of preproghrelin and ghrelin in duck . goose and emu . sense and antisense primers were designed on the basis of chicken ghrelin cdna sequence ( dest accession no . ab075215 , 836bp ) and proventriculus cdna was performed as the template Ab075215 ， 836bp )設計引物，以鴨、鵝、鴯鹋腺胃cdna為模板，通過rt - pcr及3 - race的方法，將各個產物經過回收，再經連接轉化，克隆測序，拼接后得到鴨、鵝、鴯鹋preproghrelin基因的cdna序列及ghrelin的cdna序列，并由此推測出preproghrelin及ghrelin的氨基酸序列。
In this paper , a field strain of infectious bronchitis virus was isolated from proventriculus tissue , morphological observation by electron - microscope and the biological characterizations of the virus were studied , pairs of specific primers are designed and synthesized in correspondence with them , according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes , the cdna of si gene , s2 gene , m gene . n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported 在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，并分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，并對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
K01458 , 1284bp ) , we designed the primers and regard the pcr productions as the internal standard . then by using rt - pcr analysis , high levels of expression of emu ghrelin mrna were detectable in proventriculus were detected , low levels of expression of duck ghrelin mrna were also detectable in lung muscle ileum duodenum corpus striatum cerebellum brain stem and gizzard K01458 ， 1284bp )設計引物，以其pcr產物作為內標，分別以鴯鹋各個組織反轉錄產物為模板，通過多重pcr的檢測ghrelin的mrna在鴯鹋的各個組織中表達情況，結果發現ghrelin的mrna在鴯鹋ghrelin的mrna除在腺胃特異表達外，在肺、肌肉、回腸、十二指腸、紋狀體、小腦、腦干、肌胃也有少量表達。