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protoplast n.原人;原物;原生動物;【生物學】原生質體。

protostele

This new method is basically different from the traditional way for gcv sap , which begins from the isolation of the protoplast of guard cells , and then vacuoles are isolated from protoplasts 用玻璃毛細管從液泡取樣的方法,克服了傳統方法從分離保衛細胞原生質體入手,再分離液泡的復雜、污染機會多、工作量極大等缺點。

Somatic hybrid cells were obtained between ethionine resistant cell line of astragalus adsurgens pall , and agrobacterium / “ / z / zogerae . s ' - transformed cell line of medicago saliva l . using protoplast fusion by peg method 通過peg誘導原生質體融合和雜種細胞的篩選培養,首次得到沙打旺( + )紫花苜蓿的屬間體細胞雜種。

( 2 ) when treated with iwf , the cytosolic calcium in the protoplasts from the two cultivars both increasing . but it increased significantly in the protoplasts of cv . lovrin 10 than in cv ( 2 )激發子處理后,洛夫林10和5389原生質體內ca ~ ( 2 + )濃度都有所升高,但洛夫林10原生質體胞鈣濃度要顯著高于5389原生質體。

For this it is inconvenient or disadvantageous to exploitation and applications of bifidobacterium . utilizing the technology of protoplast fusion , facultative lactobacillus ' s aerotolerant gene was integrated 本實驗以兼性厭氧乳桿菌作為遺傳物質的供體,應用原生質體融合技術來改良雙歧桿菌菌種厭氧特性。

Extro growth hormone releasing factor mediated by protoplast could more than 28 days retention in rabbit and mouse muscle that is condition of long - time expression of grf 通過提取注射部位dna后進行pcr鑒定,可以確認原生質體介導的grf基因至少可以在受試動物體內滯留28天以上,為grf的長期表達提供了條件。

The mts in protoplast of cv . 5389 also depolymerized when treated with iwf for 60 and 120 minutes , but the depolymerization was much less than that of cv . lovrin 10 而感病品種5389原生質體在激發子處理60 、 120min時,微管骨架雖也發生解聚,但其解聚程度較洛夫林10原生質體明顯輕微。

The regeneration of protoplasts could be classfied into two forms : the yeast - like cell developed from the protoplast and the mycelium grew from the protoplast directly 原生質體的再生有2種方式,一種是原生質體一端突出形成酵母出芽狀細胞;另一種是在原生質體一端直接長出菌絲。

The protoplast of ni - 5k was inactived after treated by 20w uv , 35 minute . the mycelium of t - 730 and ni - 5k were treated with mixed enzyme which including cellulase 用uv對黑曲霉ni - 5k原生質體進行滅活處理,滅活條件為:紫外線燈功率20w ,煙巨40cm ,滅活時間35分鐘。

The protoplasts of arabidopsis were isolated and the voltage - dependent k + - selective channels in the plasma membrane were recorded by whole - cell configuration O 。的產生部位。我們分離了擬南芥保衛細胞原生質體并記錄在不同處理條件下全細胞內向k ”電流的變化情況。

It indicated that the primary protoplasts of p . digitatum were released mainly from the hyphalapex , and occasionally from the subapical or original sites 此外,還對原生質體的釋放和再生方式進行了跟蹤觀察。原生質體釋放有頂端、側端和原位釋放3種方式。

The ligation mixture was used to transform s . lividans tk24 protoplasts . six colonies capable of growing in the presence of apramycin were isolated 連接液轉化標準宿主菌s . lividanstk24原生質體,最終得到6個可以在安普霉素平板上生長的陽性克隆。

All these data indicate that there may be cam binding sites in the arabidopsis protoplast and cam binding has characteristic of receptor kinetics 一系列實驗表明擬南芥原生質體質膜外側可能存在cam的結合位點,并且這種結合具有受體動力學特性。

The application of protoplast technology in genetic breeding of edible mushroom was reviewed . at the sane mw , the problems , existed and the prospects were studied 摘要主要介紹了原生質體技術在食用菌方面應用的主要技術及存在問題、發展前景。

Several kinds of regeneration medium were tested to identify the potential effects of regeneration medium on protoplast regeneration 試驗了幾種不同鏈霉菌再生培養基培養原生質體,發現優于rzye和spmr ,再生率m 。

Snailase and lysozyme . the protoplast yield of t - 730 and ni - 5k was 3 . 2 + 107 / ml and 3 . 8 + 106 / ml under the optimum conditions 以t - 730 、 ni - 5k菌株為親本,采用混合酶液處理兩親本菌株的營養菌絲,獲得原生質體。

The enzyme combination of 2 % cellulase onozuka r - 10 , 0 . 5 % hemicellulase and 0 . 5 % pectinase was effective for protoplast isolation 比較了不同培養基、培養方法和培養密度對原生質體分裂和再生的影響。

An axial filament , with a similar structure to a bacterial flagellum , is spirally wound about the protoplast inside the cell wall 在細胞壁內部,和細菌鞭毛有相同結構的軸向細絲包繞細胞質并使之螺旋化。

The highest yield of protoplasts ( 4 . 2 x 106 / g f . wt . ) was obtained from 12 - day - old calli after subculturing on fresh medium 結果表明,用轉代后生長12d的愈傷組織,分離的原生質體產率為4

The optimum mycelial age for preparation of protoplast was at 24h time - point after inoculation and culture of conidia 制備該菌原生質體的最佳菌齡為從孢子接種起培養24h 。