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protonema n.(pl. -mata ) 【植物;植物學】原絲體。a...

protonephridium

Because of the special biological structure of bryology , it was very difficult to transfer foreign gene into the protonema or gametophyte by agrobacterium - mediated transformation . protoplasts as acceptor , using direct dna transfer methods such as microprojectile bombardment and peg - mediated transformation is becoming a good way 由于蘚類植物特殊的生物學結構用農桿菌侵染其原絲體或者莖葉體很難實現轉化,以原生質體作受體是蘚類植物轉化的常用途徑。

Isolation of protoplasts from different stage of protonema and cauliform revealed that 15 - 17 days protonema was the best material because of the higher protoplasts output . there was less protoplasts when the protonema was over three weeks old ( 4 )通過對原絲體預處理不同時間觀察表明酶解前用0 . 02 tween - 80預處理30min能明顯提高原生質體的產量。

Protonema ( pl . protonemata ) the young bryophyte gametophyte that develops following spore germination . the mature gametophyte plants develop from buds that form at several points along the protonema 原絲體:苔蘚植物中直接由孢子萌發形成的結構稱為原絲體。原絲體上形成1個或幾個芽體,芽體進一步發育為配子體。