x
uploads/pronucleus.jpg

pronucleus n.(pl. -clei ) 【動物;動物學】原核。ad...

pronunciamento

The percentage of oocytes cleaved and formed pronucleus in either 0 . 625 ( ag / ml or 1 . 25ug / ml chx group was significantly higher than the control group ( p < 0 . 05 ) . however , when the concentration of chx was increased to 5 ug / ml , the cleavage rate and pronuclear formation rate were decreased significantly ( p < 0 . 01 ) 結果培養在0 . 625和1 . 25 g mlchx的卵母細胞的激活分裂率和總原核形成率明顯高于對照組( p 0 . 05 ) ,而培養在5 g mlchx的卵母細胞的激活分裂率和原核形成率則極顯著低于對照組( p 0 . 01 ) 。

Using the hprt gene as a positive control , our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist . female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too . while since the 4 - cells period , xist is constantly transcribed until blastocyst stages 然后利用實驗一確定的pcr條件,以hprt為陽性對照,用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析,結果發現,轉錄sry基因的睪丸組織以及雄性胚胎,從受精卵發育到囊胚的過程中,基本上不轉錄xist基因;不轉錄sry基因的雌性卵母細胞和雌性胚胎,從出現原核開始,到發育至2 -細胞期的過程中, xist基因一直不轉錄,但是,從4 -細胞期開始,一直到孵化前囊胚階段,雌性胚胎都轉錄xist基因。

The mice showed also ageing - associated decline in oocyte quality , as indicated by increased frequency of premature chromatid separation , spindle disruption and / or chromosome misalignments in mii oocytes of aged mice . the gv oocytes from aged mice normally matured in vitro . the percentages of pronuclear - stage embryos , 2 - cell embryos and blastocyst derived from mii oocytes of aged mice by in vitro fertilization ( ivf ) were comparable with ones of young mice , but pronuclei formed 1 h later in aged mice than in young mice 老齡小鼠gv期卵母細胞能正常體外成熟;老齡小鼠m期卵母細胞經體外受精后形成原核期胚、 2 -細胞期胚和囊胚的比率與年輕小鼠比較無顯著差異,但原核形成時間延遲1小時;在老齡小鼠卵母細胞,注射牛精子抽提物或sr ~ ( 2 + )處理時所誘發的ca ~ ( 2 + )振蕩以及在減數分裂成熟及早期胚胎中的pkc分布未發生改變。

On the other hand , - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established . methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation . this vector is used to pronucleus microinjection 本實驗以pegfp - n1質粒為骨架載體,用酶切連接的方法構建一個順序含有- actin啟動子、 fad2cdna 、 sv40polya加尾信號的真核表達載體,雙切線性化后回收,使用回收的表達載體經原核顯微注射生產轉基因小鼠。

While cortical alveoli discharge in batches , the plasma membrane has been reorganized time after time . spermaster “ elongate and traction , and cytoplasm “ flowage make the male pronucleus and the female pronucleus conjugate )十物’ i :他地仰排原核紀合是精人己光擴悵、引和細w質流動兒d 。