P - pokeweed 中文 - EN166

pokeweed n.【植物；植物學】美國商陸 (Phytolacca a...

pokey
Sds - page results showed that as to mut + recombinant highest yield was obtained after 4 days inducing and with the culture time prolonged it reduced . pokeweed antiviral protein gene expressed well when methanol concentration reached 10g / l . pokeweed antiviral protein obtained high yield in thin acidic culture medium ( ph6 . 0 - 6 . 4 ) and its quantity in total mass of secrete protein exceeded 30 % Sds - page分析結果表明， mut ~ +組菌株在甲醇誘導第四天后pap在培養液中積累量達到最高水平，延長培養時間會導致產量下降；在10g / l的甲醇濃度誘導下， pap的表達量達到最高；培養基ph值在偏酸性條件下( 6 . 0 - 6 . 4 ) pap的表達量都維持在較高的水平。
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector . the sequencing results showed that pap gene had 99 . 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ) . the iptg - inducible expression vector containing the pap gene was constructed and transferred into e . coli bl21 ( de3 ) - plyss 將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然后采用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那霉素抗性篩選，最后獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒( tmv ) ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115 . mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting 本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
To find out the difference between mut + and muts recombinants we compared their expression of pokeweed antiviral protein in the same conditions 在相同的培養條件下比較了mut ~ +和mut ~ s重組菌株表達pap的異同。
Many studies revealed that pokeweed antiviral protein ( pap ) can control plant virus effectively 許多研究發現美洲商陸抗病毒蛋白( pokeweedantiviralprotein ， pap )對植物病毒具有良好的控制效果。
Someone tried this way but the yield is low . francis expressed truncated mutant pokeweed antiviral protein gene in pichia pastoris 目前已經有人在這方面做出了嘗試，但蛋白表達產量還不高。
The blackish - red berry of the pokeweed 美洲商陸的黑紅色漿果。