x
uploads/pili.jpg

pili n.〔Tag.〕1.卵橄欖。2.卵橄欖樹 (Canari...

For oversea pili fans who wish to join the free epiil membership , please fill the “ oversea epili member application “ 海外會員加入霹靂網請利用霹靂網海外會員申請表。

pili-

In this study , the meaningful results have been achieved , which is the important basic work to research the pili subunit vaccine of avian e . coli , and detect pila gene by nucleric acid probe . moreover , it is significant to research molecular epidemiology , to diagnose , to prevent and treat avian colibacillosis 本研究為雞源致病性大腸桿菌菌毛基因工程疫苗的研究,制備核酸探針檢測pila基因提供了重要材料,對雞大腸桿菌病的分子流行病學研究、診斷和防治研究具有重要意義。

In this paper , 45 e . coli strains isolated from chicken farms in sichuan province were determined to be the pathogenic e . coli by animal test . type 1 pili of 45 strains isolated was detected by msha . the pila gene of 45 avian pathogenic e . coli strains were amplified by the polymerase chain reaction ( pcr ) with primers designed according to the sequence of the pila gene in genbank . results showed that pcr was more sensitive , faster and more characteristic than msha to detect type 1 pili 本研究將從四川規模化雞場分離鑒定、經1日齡雛雞致病性試驗得到的雞源致病性大腸桿菌45株,采用d -甘露糖敏感血凝試驗( msha )檢測1型菌毛,根據genbank中公布的人源大腸桿菌1型菌毛pila基因序列設計一對引物用pcr擴增雞源致病性大腸桿菌1型菌毛pila基因。

The result showed that the homology rate of pila gene among the 5 avian pathogenic e . coli strains tested and one human e . coli were from 89 . 8 % to 91 . 1 % , and the homology rate of amino acid were from 88 . 5 % to 91 . 8 % . the homology rate of pila gene sequence among 5 avian pathogenic e . coli strains tested and avian pathogenic e . coli reported ( serotype o1 , o2 , o78 ) were from 87 . 8 % to 90 . 2 % , and the homology rate of amino acid were from 84 . 6 % to 91 . 2 % . there had homology in avian pathogenic e . coli . there had some common antigen side in type 1 pili of avian pathogenic e . coli 結果表明:運用msha法檢測1型菌毛的檢出率為80 ( 36 45 ) , pcr法的檢出率為95 . 5 ( 43 45 ) , pcr方法用于1型菌毛的檢測比msha更加敏感、快速、特異性強;選擇5株優勢血清型雞源致病性大腸桿菌代表株( o _ ( 89 ) , o _ ( 119 ) , o _ ( 141 ) , o _ ( 127 ) )的1型菌毛pila基因的pcr擴增片段經純化后,分別定向克隆到puc18質粒的多克隆位點,構建了含有目的基因片段的克隆質粒,并轉化到dh5株大腸桿菌載體菌中,篩選獲得陽性克隆菌株。

It should meet the following 3 requirements : being set up in line with relevant legal basis , having its full name , organization and location , and capable of taking civil liabilities ; independently possessing and using its assets and assuming its liabilities , and entitled to sign contracts with other institutions ; and making independent accounts of its profits and losses , and capable of com pili ng its own balance sheet 它應同時具備的條件是:依法成立,有自己的名稱、組織機構和場所,能夠承擔民事責任;獨立擁有和使用資產,承擔負債,有權與其他單位簽訂合同;獨立核算盈虧,能夠編制資產負債表。

Type 1 pili is the important virulence factors on the e . coli in fection in chicken . through the adhering of pili , e . coli adhered on the epidermic cell of aspiratory tract , which was the first step of invading in host 1型菌毛是雞源致病性大腸桿菌的重要毒力因子,在致病過程中介導細菌吸附于雞呼吸道粘膜上皮細胞完成入侵的第一步。

Healthy rabbits were inoculated each with vaccine of the recombinant fused pili - el - 2 protein at 250ul per dose to potentiate the immunogenicity . rabbits inoculated two times at one week interval 擴增此質粒,回收bamh切出的2 . 5kb的基因片段,與pme290 ( bamh酶切后去磷酸化)載體連接。

We currently do not offer pili store direct online order for the oversea members , for the pili dvd enquiry please check this page 目前霹靂網商品館并無提供直接的線上訂購服務給霹靂網的海外會員,希望訂購霹靂劇集珍藏dvd的朋友請前往相關說明頁面查詢。

D . nodosus pili is the main protective immunogen against footrot in ruminants . il - 2 is known to be as an adjuvant in many areas 已有很多實驗證明il - 2作為佐劑能提高體液免疫和細胞介導的免疫反應。

It also means that the il - 2in the fused pili - il - 2 construct may also have a potential role in stimulating vaccine antigen 利用提取的表達產物進行家兔免疫實驗,結果表明該產物具有免疫原性。

For oversea pili fans who wish to join the free epiil membership , please fill the “ oversea epili member application “ 海外會員加入霹靂網請利用霹靂網海外會員申請表。