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phenylalanine

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phenylalanine n.【化學】苯基丙胺酸。

n. 【化學】苯基丙胺酸。 “benzyloxycarbonyl phenylalanine“ 中文翻譯：   下氧基碳酰苯丙氨酸“d-phenylalanine“ 中文翻譯：   苯丙氨酸“dimethyl-phenylalanine“ 中文翻譯：   二甲基-苯丙氨酸“hydroxymethyl phenylalanine“ 中文翻譯：   羥甲基苯丙氨酸“isopropyl-phenylalanine“ 中文翻譯：   異丙基-苯丙氨酸“l phenylalanine“ 中文翻譯：   鳥氨酸鹽酸鹽“l-phenylalanine“ 中文翻譯：   苯丙氨酸; 苯基丙氨酸“nitro phenylalanine“ 中文翻譯：   硝基苯基丙氨酸“phe-phenylalanine“ 中文翻譯：   苯丙氨酸“phenylalanine acetyltransferase“ 中文翻譯：   苯丙氨酸乙酰轉移酶“phenylalanine agar“ 中文翻譯：   苯丙氨酸瓊脂“phenylalanine aminotransferase“ 中文翻譯：   苯丙氨酸氨基轉移酶“phenylalanine and methotrexate“ 中文翻譯：   苯丙氨酸和氨甲蝶呤“phenylalanine deaminase“ 中文翻譯：   苯丙氨酸脫氨酶“phenylalanine decarboxylase“ 中文翻譯：   苯丙氨酸脫羧酶“phenylalanine hydroxylase“ 中文翻譯：   苯丙氨酸羥化酶“phenylalanine measurement“ 中文翻譯：   苯丙氨酸測量“phenylalanine monooxygenase“ 中文翻譯：   苯丙氨酸單加氧酶; 苯丙氨酸加單氧酶“phenylalanine mustard“ 中文翻譯：   苯丙氨酸氮芥; 苯基丙氨酸氮芥, 溶肉瘤素“phenylalanine nitrogenmustard“ 中文翻譯：   苯丙氨酸氮芥“pyridoxylidene phenylalanine“ 中文翻譯：   吡哆苯丙氨酸“chloroacetyl-dl-phenylalanine“ 中文翻譯：   氯乙酰苯丙氨酸“d-phenylalanine mustard“ 中文翻譯：   右旋苯丙氨酸氮芥; 右旋丙氨酸氮芥“disorder of phenylalanine metabolism“ 中文翻譯：   苯丙氨酸椿病癥; 苯丙氨酸代謝病癥“phenylalanine 4-hydroxylase“ 中文翻譯：   苯丙氨酸4-羥化酶“phenylalaninase“ 中文翻譯：   苯丙氨酸酶; 苯基丙氨酸酶

In escherichia coli , arog gene encodes phenylalanine - sensitive 3 - deoxy - d - arabino - heptulosonate - 7 - phosphate synthase isoenzyme arog that catalyzes the first committed step of shikimate pathway . here we study the essential amino acid residues involved in the formation of feedback inhibition site of arog , and the effects of n - terminus on feedback inhibition and its quaternary structure , and the importance of the structural “ d2 “ symmetry to allosteric inhibition 本博士論文工作以大腸桿菌k - 12來源的arog為研究對象，通過定點突變、反饋抑制實驗和酶學動力學參數的測定，深入地研究了arog的反饋抑制位點的特性，并對arog的n -末端在反饋抑制機理和維持穩定四級結構中的作用，以及蛋白質結構的“ d2 ”對稱性對酶功能的重要性等進行了具體的研究。

In escherichia coli , arog gene encodes phenylalanine - sensitive 3 - deoxy - d - arabino - heptulosonate - 7 - phosphate synthase isoenzyme arog that catalyzes the first committed step of shikimate pathway . here we study the essential amino acid residues involved in the formation of feedback inhibition site of arog , and the effects of n - terminus on feedback inhibition and its quaternary structure , and the importance of the structural “ d2 “ symmetry to allosteric inhibition 本博士論文工作以大腸桿菌k - 12來源的arog為研究對象，通過定點突變、反饋抑制實驗和酶學動力學參數的測定，深入地研究了arog的反饋抑制位點的特性，并對arog的n -末端在反饋抑制機理和維持穩定四級結構中的作用，以及蛋白質結構的“ d2 ”對稱性對酶功能的重要性等進行了具體的研究。

phenylamine

Activity of alcohol dehydrogenase and phenylalanine ammonia - lyase varied during the blooming of jasmine flower . the non - specific esterase isozymes were isolated by different methods . two enzyme stripes were gained with page ( polyacrylamide gel electrophoresis ) method , while eight stripes were obtained with ief ( isoelectrofocusing ) method 等電聚焦法分離茉莉花酯酶的效果較好，用聚丙烯酰胺常規分離只得到2條酶帶，而用等電聚焦法分離茉莉花酯酶同工酶可獲得8條比較清晰的酶帶。

Our work elucidates the formation of the feedback inhibition site . point mutations reveal that amino acid replacements at phel44 , leul75 , leul79 , phe209 and val221 partially or completely relieve the feedback inhibition of arog addressed by 1 mm phenylalanine . combined with the 3d structure of arog , our results demonstrate that the feedback inhibition site consists of asp6 , asp7 , ile10 , ile13 , pro150 , gln151 , leul75 , leu179 , serl80 , phe209 , ser211and val221 實驗結果表明：在1mm苯丙氨酸的存在下， 1 )野生型arog受到90以上的反饋抑制； 2 ) pro150leu , leu179ala , phe209ala和val221ala這四種點突變在保持與野生型arog相近的比活的同時，解除了80 100的反饋抑制； 3 )點突變phe144ala僅解除反饋抑制約30 ，同時保持與野生型相當的比活； 4 )突變體leu175ala僅能夠解除18的反饋抑制， leul75gln能夠解除44的反饋抑制， leu175asp解除了84的反饋抑制，并且比活都比野生型高。

The content and source of protein and the composition of amino acids are important in artificial feed , because the ingredients of most feed raw material lack arginine , l - lysine and phenylalanine . high levels of growth , food conversion and survival were obtained with a diet containing 31 . 5g arginine kg diet , l - lysine 26 . 0 kg diet and phenylalanine 14 . 0 kg diet in shrimp culture ：飼料的配制，除考慮蛋白質含量還考慮蛋白質來源和原料氨基酸的組成，因飼料原料大致上缺乏離氨酸，精氨酸和苯丙氨酸如幼蝦飼料中每公斤含

Phenylketonuria ( pku ) is an inherited metabolic disease that results in mental retardation and other neurological problems when treatment is not started within the first few weeks of life . the disease arises from the deficiency of a single enzyme , phenylalanine hydroxylase , which converts the essential amino acid , phenylalanine , to another amino acid , tyrosine . failure of the conversion to take place results in a buildup of phenylalanine in the body that then damages the central nervous system 苯丙酮尿癥（ pku ）是一種智力發育不全的先天性疾病，患者由于肝贓內苯丙氨酸羥化酶缺乏，苯丙氨酸不能正常代謝為酪氨酸，從而導致苯丙氨酸在肌體組織內積累，引起腦損傷和累進性精神障礙，臨床表現為智力低下,頭發顏色轉黃，尿有異臭味，重者似鼠臭。

The quinolone resistance - determining region ( qrdr ) of gyra gene of 19 resistance isolates was amplified by pcr , sequenced and their amino acid mutations were analyzed . the results revealed that 9 isolates contained a mutation in gyra of a serine at position 83 ( ser83 ) to a phenylalanine , and 1 isolate exhibited a mutation of ser83 to a tyrosine , and 6 isolates that were resistant to more than six kinds of fluoroquinolones showed a mutation asp87 to asparagine at the same time 采用pcr技術擴增了對喹諾酮類藥物耐藥的19株禽源沙門氏菌分離菌株gyra基因qrdr ，經純化、測序和序列分析表明， 19株耐藥菌株中有10株第83位氨基酸發生了變異，其中有9株由絲氨酸( s )變異為苯丙氨酸( f ) ， 1株由絲氨酸變異為酪氨酸( y ) 。

The 1 : 1 inclusion complexes of - cyclodextrin with phenylalanine and tyrosine were formed ; but 2 : 1 complex was afford from - cyclodextrin with tryptophan owing to the various sites and chaps of different amino acids . the inclusion behaviors of - cyclodextrin and its polylactide - garfted copolymer with insulin were also studied via ultraviolet and fluorescent spectrometry 隨著?環糊精用量的增加和作用時間的延長， ?壞糊精對芳香族氨基酸的包合作用增強，揭示了?環糊精與芳香族氨基酸包合物在水溶液中的形成是一動態平衡過程。

In escherichia coli , arog gene encodes phenylalanine - sensitive 3 - deoxy - d - arabino - heptulosonate - 7 - phosphate synthase isoenzyme arog that catalyzes the first committed step of shikimate pathway . here we study the essential amino acid residues involved in the formation of feedback inhibition site of arog , and the effects of n - terminus on feedback inhibition and its quaternary structure , and the importance of the structural “ d2 “ symmetry to allosteric inhibition 本博士論文工作以大腸桿菌k - 12來源的arog為研究對象，通過定點突變、反饋抑制實驗和酶學動力學參數的測定，深入地研究了arog的反饋抑制位點的特性，并對arog的n -末端在反饋抑制機理和維持穩定四級結構中的作用，以及蛋白質結構的“ d2 ”對稱性對酶功能的重要性等進行了具體的研究。

Activity of phenylalanine ammonia - lyase ( pal ) was increased by uv - b radiation , and as a consequence , uv - absorbing compounds were accumulated . it was inferred from our results that the accumulation of uv - absorbing compounds such as flavonoids may be a common response for plants in resistant to enhanced uv - b radiation 在uv - b處理下，紫萍的苯丙氨酸氨基裂解酶( pal )活性上升，并導致其體內吸收uv - b的物質積累，推測類黃酮積累可能是植物抵御uv - b逆境的一個共同效應。

Puc18 - 169 was a subcloned plasmid containing the whole operon sequence of l - hydantoinase from arthrobacter bt801 which can convert 5 - benzylhydantoin to l - phenylalanine . hydantoin hydrolase which is responsible for the ring opening of hydantoin is one of the components of hydantoin utility enzymes of arthrobacter bt801 . n - carbamoylase is a part of hydantoinase operon which can transform n - carbamoylamino acids into the corresponding amino acids 節桿菌( arthrobacter ) bt801是由軍事醫學科學院生物工程研究所保存的l -乙內酰脲酶產酶菌株， puc18 - 169是由本室構建的含有節桿菌bt801的l -乙內酰脲酶完整操縱子序列的亞克隆質粒。

The product chorismate of this pathway is precursor to the synthesis of the aromatic amino acids phenylalanine , tyrosine , and tryptophan as well as other essential aromatic secondary metabolites such as ubiquinone , menaquinone and vitamin k . phosphoenolpyruvate and shikimate 3 - phophate are converted to 5 - enolpyruvlshimimate - 3 - phosphate and phosphate 芳香族氨基酸苯丙氨酸、酪氨酸、色氨酸和其它一些必需代謝物如泛醌、維生素k2類、維生素k的起始物是莽草酸途徑所產生的分枝酸。

The pocket accommodating the aromatic ring of phenylalanine is formed predominantly by hydrophobic side - chains , including those of ile10 , ile13 , prol50 , leul75 , leul79 , phe209 , ser211 and val221 ; whilst the amino group and carboxylate group of phenylalanine is coordinated by asp6 , asp7 , gln151 and ser180 . the n - terminal region is found to be important to the function of arog . point mutation reveals that residue substitution of ilelo to ala10 leads to desensitization in the presence of 1 mm phenylalanine , simultaneously , exhibits a dramatic decrease in enzymatic activity 綜合前人的研究和本研究工作的實驗數據，得出以下結論： 1 )在arog的反饋抑制位點供苯丙氨酸結合的疏水口袋由asp6 、 asp7 、 ile10 、 ile13 、 pro150 、 gln151 、 leu175 、 leu179 、 ser180 、 phe209 、 ser211和val221等12個氨基酸殘基構成； 2 )苯丙氨酸結合的疏水口袋分為兩個區域，與苯丙氨酸的苯環發生疏水作用的區域由ile10 、 ile13 、 pro150 、 leu175 、 leu179 、 phe209和val221的側鏈構成，而asp6 、 asp7 、 gln151和ser180的側鏈構成了與苯丙氨酸的氨基和羧基相互作用的區域。

Multi - copies insertion transformants were screened on g418 plates . the recombinant protein was proved to have biological activity of hydrolyzing n - carbamoylphenylalanine into phenylalanine through enzyme activity assay . the n - carbamoylase activity of recombinant was 2 . 26 and 2 . 15 times higher than that of arthrobacter bt801 and dh5a / puc18 - 169 將hyucdna片段連接到真核表達載體ppic3 . 5k上，經bgl酶切線性化，通過peg法轉化導入畢赤酵母gs115感受態細胞，利用g418抗性篩選得到12個插入多拷貝目的基因的轉化子。

The results show that the optimal carbon source of y3 is glucose and the optimal concentration of phenylalanine , tyrosine , ammonium acetate is respectively 100mg / l , 9mg / l and 20mg / l ; hydrogen peroxide can distinctly promote the producing of taxol . under the condition of addition concentration of hydrogen peroxide is 8mmol / l every 8 hours , the yield of taxol get to 700ug / l , over 9 times higher than the contrast 結果表明y _ 3菌株的最適碳源為葡萄糖；苯丙氨酸的添加濃度為100mg / l ，酪氨酸的添加濃度為9mg / l ，乙酸銨的添加濃度為20mg / l ；過氧化氫對y _ 3菌株產紫杉醇有明顯的促進作用，當以8h為時間間隔，添加濃度為8mmol / l時，紫杉醇的含量可達700 g / l ，比對照提高9倍以上。

The inclusion behaviors of b - cyclodextrin with some aromatic amino acids such as l - phenylalanine , l - tyrosine and l - tryptophan were studied via ultraviolet and fluorescent spectrometry . the results indicated that inclusion complexes of - cyclodextrin with aromatic amino acids could be formed and the inclusion behaviors were affected by the amount of - cyclodextrin , temperature and ph value 本文研究了?環糊精與芳香族氨基酸l ?苯丙氨酸、 l ?酪氨酸和l ?色氨酸之間的包合作用，考察了?環糊精用量、溫度、時間和ph值等因素對包合作用的影響。

The study of isoflavones in leaves with the development of seeds showed that the content of isoflavones in leaves was much less than that in seeds . the correlation analysis showed there was marked negative correlation between them two . the activity of phenylalanine ammonia - lyase ( pal ) in different organ before seeding has been studied 種子發育時期，種子中異黃酮含量與葉片中異黃酮的含量呈負相關，達到極顯著水平，表明種子發育過程中，葉片中的異黃酮是逐漸向種子中轉移的。

Several enzymes that indirectly affect pep are also regulated by csra . thus , a csra mutation not only causes a significant elevation in intracellular pep , but also breaks cellular intrinsic metabolic coordination and channel more carbon flux to phenylalanine biosynthesis 另外，一些間接影響pep的酶也被csra所調控，因此csra的敲除不但可以使細胞內的pep的量大大增加，而且打破了細胞固有的生理代謝協調，使碳代謝流盡可能多的流向苯丙氨酸的生物合成方向。

These results unambiguously demonstrate that a single amino acid residue substitution is sufficient to generate a phenylalanine - insensitive form of arog . furthermore , the mutant a ( l - 15 ) lacking the first fifteen n - terminal amino acid residues exhibits no sensitivity toward phenylalanine in a range of 0 ~ 1 mm phenylalanine 這些結果揭示: 1 ) n一末端在反饋抑制機理中具有重要作用; 2 ) n一末端的缺失使得ar0g緊密二聚體結構解聚，導致四級結構破壞。