P - perfuse 中文 - EN166

perfuse vt.灌，使充滿；鋪滿，灑遍，撒滿。 perfuse a...

perfusion
Material and methods normal rats of male sd were divided into young , adult , and aging groups . preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0 . 5ml / 100g body weight ) . perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50 - 100 ml and then 4 % paraformaldehyde pbs 100 - 400ml were perfused through the left ventricle of the heart , the whole procedure was lasted for about somin . the entire brain was dissected out and dipped in the fixative solution for 12h at 4 . brain pieces targeted were choosen and then passed the graded alcohols for dehydration , dipping into paraffin for embeding , and reshaping the pieces 2 ）磷酸緩沖液100400m ， 30分鐘灌注完畢，取出整腦，在上述固定劑oc ）內后固定12小時。切取觀察部位腦塊，然后，進行梯度酒精脫水，浸蠟，包埋，修塊，石蠟連續切片（德國leica石蠟切片機人切片厚度still ， zlllll ，蛋自甘油載片撈片， 60c烤箱過夜，二甲苯脫蠟，梯度酒精置換，浸水， h六染色，梯度酒精脫水，二甲苯透明，中性樹脂封片。室溫風干后，顯微鏡觀片， olympus萬能顯微鏡照相。
Objective : to establish a rat model of orthotopic gastric isotransplantation by using microsurgical techniques . methods : 70 sd rats were used in our experiment and 35 gastric trasplantations were carried out . in the donor ' s operation : after the spleen was resected and the proper liver artery was ligated , the stomach was perfused through the abdominal aorta . then the stomach was resected with its peripheral blood vessels including celiac trunk and the portal vein , etc . in the recipient operation : after the stomach and the spleen were reseeted , the implantation was performed by the following sequence : the end - to - side anastomosis between the portal veins . the end - to - end anastomosis between the celiac trunk and the left gastric artery . open the blood flow to observe the effect of the blood supply of the stomach . the end - to - end anastomosis between the duodenum . the end - to - end anastomosis between the cardiac and the esophagus . results : 35transplantations were carried out in which the operation success rate in the last 20 cases was 80 ( 16 / 20 ) . the average operation time was 2 . 35 h . the longest survival time was over three months . conclusions : the model of orthotopic gastric transplantation in rat was successfully established . it could be used to study the transplanted stomach in the abdominal multiviseeral transplantation and the reconstruction after the total gastrectomy 目的應用顯微外科技術,建立大鼠原位異體胃移植模型.方法70只sd大鼠,行35例次的胃移值手術.供體手術,先切除脾臟,經腹主動脈行原位胃冷灌洗.將胃及其所屬血管,包括腹腔干和門靜脈干等一并切取.受體手術,先切除胃和脾臟,分別行供、受體間門靜脈的端側吻合,供體腹腔干與受體胃左動脈的端端吻合,然后開放血流.再行供體和受體十二指腸間端端吻合,賁門與食管端端吻合.結果在施行的35例手術,后20例中有16例成功,成功率為80 .最長存活者達3個月.結論成功地建立了大鼠原位異體胃移植類型.該模型可用于腹部多臟器移植中移植胃的相關研究及全胃切除術后代胃的研究
Half animals were perfused with paraformaldehyte following normal serum after test innnediatly , then removed the brain and fixed for 24 hours with paraformaldehyte , paraffin sections were prepared at 3um , histochemical staining for counting ce11 pro1iferating ratio and in situ hybridization for calculating integra1 scores of stem cell factor inrn positive signals 結果如下： 1增殖細胞的觀察經72h的快眼動睡眠剝奪，實驗組大鼠海馬齒狀回區brdu與p皿a （ proliferatingcellneuclearantigen ， pma ）陽性標記細胞數顯著增加。
Abstract : it is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano - electrical feedback ( mef ) . in the present study a ventricular pressure - clamping system has been developed , which can be applied to isolated - perfused rabbit hearts . controlled by a computer , this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition , but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase . this system integrates multiple functions , including perfusing , pacing , recording of electrocardiogram and monophasic action potentials , and clamping and measuring of ventricular pressures in isolated - perfused hearts . thus , it is a distinct system for investigating the phenomena and mechanisms of cardiac mef at organ level 文摘:在心臟機械電反饋的研究中準確控制機械刺激是非常重要的.本研究室構建了一套適用于離體家兔心臟的心室壓力鉗系統.該系統通過計算機控制壓力鉗,不僅能模擬正常生理條件下左心室的壓力波形,還能在心室活動周期的特定時相、以適當波形對心室施加機械刺激.該系統集心臟灌流與起搏、表面心電圖記錄、單相動作電位記錄、心室壓力鉗制與測定等多種功能于一體,特別適用于器官水平上觀察機械電反饋現象并探討其機制
It is necessary to control the mechanical stimuli precisely in the studies of cardiac mechano - electrical feedback ( mef ) . in the present study a ventricular pressure - clamping system has been developed , which can be applied to isolated - perfused rabbit hearts . controlled by a computer , this system not only can make the left ventricle follow a command defining the same pressure wave as that during a beating cycle under physiological condition , but also deliver mechanical stimuli with a proper waveform to the ventricle at a particular time phase . this system integrates multiple functions , including perfusing , pacing , recording of electrocardiogram and monophasic action potentials , and clamping and measuring of ventricular pressures in isolated - perfused hearts . thus , it is a distinct system for investigating the phenomena and mechanisms of cardiac mef at organ level 在心臟機械電反饋的研究中準確控制機械刺激是非常重要的.本研究室構建了一套適用于離體家兔心臟的心室壓力鉗系統.該系統通過計算機控制壓力鉗,不僅能模擬正常生理條件下左心室的壓力波形,還能在心室活動周期的特定時相、以適當波形對心室施加機械刺激.該系統集心臟灌流與起搏、表面心電圖記錄、單相動作電位記錄、心室壓力鉗制與測定等多種功能于一體,特別適用于器官水平上觀察機械電反饋現象并探討其機制
D ) pain and electroacupuncture stimulated group : after the injection of 200ul formaldehyde ( 5 % ) in the right hind paw , the rats are stimulated with electroacupuncture immediately ; the four groups of rats were perfused . sections were cut at 40um for the method of nadph - d 疼痛刺激加電針刺激組，在大鼠右側后腳掌注射5甲醛200ill后立即固定，并用針灸治療儀刺激同側后肢“足三里”穴3v5shz ，持續0
The isolated hepatocytes , isolated perfused organs , tissue slices , liver cell lines , subcellular fractions and the genetically engineered cells are the most commonly used in vitro liver models nowadays , especially the isolated hepatocytes 目前，常用的肝臟體外模型包括原代肝細模型、離體肝臟模型、肝臟切片模型、肝細胞系模型、亞細模型及基因工程細模型等，其中原代肝細型最?常用。
Methods the arteries of the heads of pancreas of 15 adult corpses were perfused with tincture and fixed with formaldehyde for the investigation into the direction and the distribution of the vessels in the 15 cases ' pancreas - duodenal molten specimen 方法對15例染色劑灌注甲醛固定的成人尸體胰頭區的動脈血管進行解剖研究，觀測15例胰十二指腸鑄型標本的血管走行、分布。
Amiodarone prevent electrophysiological changes of acute atrial dilation accompanied with rapid atrial stimulation in isolated langendorff - perfused rabbit heart 離體兔心臟急性雙心房擴張合并快速心房刺激時電生理變化及胺碘酮的干預作用
The heart was perfused with a glutin solution of 30 % concentration . after that it was embedded with two steps to guarantee its anatomical position 以30 %濃度的明膠溶液灌注心臟，分兩步進行包埋，以保證心臟處于解剖位。
Adequate quantities of plasma continued to perfuse the kidney . 相當量的血漿持續灌注到腎臟。