O - orf 中文

orf n.【魚類】黑色金魚。

orfe
Sds - page and activity staining analysis revealed that all of clones 2 , 5 and 9 exhibited a single active band with 83 - kd in size , whereas clone 3 exhibited four different bands with 64 kd , 70kd , 76kd and 83 kd after construcing a series of deleted subclones from the plasmid prepared from clone - 5 , a complete nucleotide sequences comprising of 3 , 205 bps wes determined . one open reading frame ( orf ) was found in this sequence , which was comprised of 2 , 661 bps and could encode a polypeptide with 887 amino acid residues 利用基因分析軟件genetyx對clone - 5質粒的核苷酸序列進行分析，并搜索日本dna數據庫( dnadatabankofjapan ，簡稱ddbj ) ，推斷clone - 5質粒的幾丁質酶基因含有一個2 ， 261bp的開放閱讀框( orf ) ，編碼一條887個氨基酸的幾丁質酶蛋白； orf上游有一個啟動子。
Goat anti - human ige antibody were used as second antibody to make sure that the positive clones were ige related . through three cycles of screening , the inserted cdna fragments of the positive clones were amplified by pcr and sequenced . the results showed that the inserted cdna fragment from one clone was 1200 bp in length , with a orf of 507 bp which encoded 169 amino acids Sj43b pgex 6p 1重組質粒的誘導表達、表達產物的鄉寸和免疫學性質鑒定分析為獲得可溶性的rsj43b月6gsta蟲合蛋白，對不同iptg誘導劑濃度、誘導表達溫度和誘導表達時間等因素對融合蛋白可溶性表達的影響進行了觀察。
The cdna sequence with 789 bp encodes an uncompleted open reading frame ( orf ) of 263 amino acids , which included a signal peptide of 18 amino acids , a proposed propeptide of 171 amino acids , a partial mature protein of 74 amino acids . the sequences of signal peptide and propeptide are very similar to ones from wannan agkistrodon acutus reported by fan chunyan , but it only contains partial amino acids of mature protein . the 800 bp is likely to be a part of gene encoding metalloproteinase agkistrodon acutus from guangxi province 結論： 600bp產物和已報道的皖南五步蛇纖溶酶金屬蛋白酶氨基酸序列相比較，有90 . 6 %的同源性； 800bp產物中信號肽及前肽和已報道的皖南五步蛇金屬蛋白酶很相似，同源性為97 . 9 % ，但僅含有部分金屬蛋白酶成熟肽的氨基酸序列，推測此片段為廣< wp = 6 >西五步蛇金屬蛋白酶原基因的一部分。
The 496 bp fragment of the orf of p22 gene and a 561 bp fragment were amplified from the genomic dna of zs strains of toxoplasma gondii . both 496 bp and 561 bp fragments were successfully cloned into the plasmid pthiohisa , b , c and pbudce 4 . 1 respectively . 2 從弓形蟲zs株基因組中擴增出p22編碼基因的一長496bp ，另一長561bp的片段，并成功構建含p22編碼基因的原核質粒重組體pthiohisa ， b ， c / p22 ，及真核重組表達質粒pbudce4 . 1 / p22 。
Lividans strains with dnd + and dnd “ phenotype were used for testing phage infections , growth under different ph , temperature , concentration of nacl and an extremely low concentration of o2 . no obvious differences could be tested from these strains 它們均攜帶包含3個orfs的區域(除了phz2153在dndb和dndc內缺失了2kb ) ，但已分別在各orf上發生單基因中斷( phz2150 ， phz2151和phz2152上外源片段的中斷分別發生在dnda ，月和c上) 。
713bp and 700bp specific fragments were amplified by pcr and ligated into pgem - t easy vector . it was identified by restriction endonuclease digest analysis , pcr and sequencing that this fragment contained the complete open reading frame ( orf ) of the hc and ha gene 擴增產物連接到pgem - teasy載體上，轉化入大腸桿菌jm109中進行藍白斑篩選后，用酶切、 pcr鑒定和測序的方法鑒定出重組陽性質粒( pgem - hc和pgem - ha ) 。
A search for database in the dna data bank of japan ( ddbj ) revealed that the amino acid sequence of the above orf shows 90 % identity with that of one putative chitinase from aeromonas hydrophilu in which only a primary sequence has been reported to date 經檢索，與ddbj中登陸的一個來源于aeromonas的幾丁質酶基因有90的同源性。另一方面，在生物遺傳轉化的研究中，理想報告基因的探索一直是重要研究內容之一。
Vp2 and vp3 . the genes , encoding them , were located in a same orf , and they possess of common stop codons . furthermore , amino acid sequence of vpl contained the fu1l sequence of vp2 and vp3 , so vp1 gene become interest gene . in this research Gpv的三種結構蛋白vp1 、 vp2 、 vp3位于同一開放閱讀框內，共用同一終止密碼子， vp1包含了組成vp2 、 vp3的所有氨基酸，因其具有這一特殊性，成為本研究的目的基因。
Sequences flanking tn5 in nm21 was cloned by anchored pcr . a 3073bp fragment which contains three putative open reading frames ( orf ) and shows strong homology with the magnetospirillum magnetotacticum ms - 1 was obtained , the tn5 inserted in the orf1 用錨定pcr法從nm21中克隆出tn5側翼序列，得到一個大小為3073bp的dna片段，此片段由3個orf組成，與m . magnetotacticumms - 1的對應片段具有89的同源性。
The tk gene contained an open reading frame of 957 bp encoding a 318 aa protein . upstream of the tk orf , three putative gc boxes are located at positions - 22 , - 166 and - 199 . a potential poly a signal begins 110 nucleotides downstream from the termination codon at position 1306 在tkorf上游- 22 、 - 166 、 - 199位存在3個gc框樣序列，在終止密碼子下游第110個核苷酸處的1306位存在有多聚腺苷加尾信號。
At present we usually use detecting bdv orf ii genome to cofirm whether the people have a bdv infection or not , the research suggests that bdv orf ii is more conservative and less variability , thus it is a good marker for detecting bdv infection 目前為證實是否存在bdv感染一般采用對其orf基因片段( p24 )的檢測，研究結果表明此序列較保守、變異性小，是檢測病毒感染的特異性標志物。
It was reported that cadherin - like receptors are most likely the main receptors of bt toxic protein . bt - r3 , a gene of 5551 , is a newly cloned cadherin - like receptor gene and its orf ( open reading frame ) encodes 1715 amino acids Bt - r3受體基因是吳志平博士在其博士論文研究中新克隆到的鈣粘蛋白類受體基因，它的cdna序列全長5551bp ，編碼1715個氨基酸殘基組成的bt - r3前體蛋白。
Marek “ s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ) . then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector 本研究將型mdvmd11株的pp24基因的完整orf克隆入原核表達載體pgex - 6p - 1中，重組質粒pgex - pp24轉化bl21宿主菌后，經iptg誘導表達。
The complete human znf359 cdna sequence is 3270 bp and contains a 1932 bp open reading frame ( orf ) that encodes a 643 amino acid protein with an n - terminal krab domain and 16 c - terminus zinc finger c2h2 motifs Znf359基因長3271個堿基，含6個外顯子，在基因組dna上長18kb 。它編碼的蛋白質全長643個氨基酸，含一個krab框， 16個c2h2型的鋅指，在兩者之間還有一個典型的中間重復序列。
2 the polymorphism of ul136 gene dna and amino acid sequence 1 ) the length of ul136 orf in all 18 clinical isolates was similar to that of toledo , 723 bp in size . they hade the potential to encode 241 amino acid protein ? 2 ?二月136基因編碼區及編碼產物氨基酸序列的多態性株臨床低傳代分離株ul136orf長度均與toedo株相同，為723hp ，預測編碼241個氨基酸的蛋白。
The nucleotide sequence of this 1 . 5 kb dna fragment was determined , and one orf was found and named as red gene , which was comprised of 771 bp and coded for a polypeptide of 257 amino acid residues in size 測定各亞克隆的核昔酸序列，確定red伽i sa的插入片段為1 ， 54fop 。利用軟件分析，并搜索ddbj ，推斷redgene的開放閱讀框為77fop ，編碼257個氨基酸。
8kb cdna with 1362bp orf and codes 454 amino acids with only a sh2 domain . the gene was named as sh2a at chromosome 8p22 . we study its structure by blast homologous analysis and its expression by rt - pcr and northern blot 本文將通過蛋白質分析軟件研究其結構和同源性，利用rt - pcr和northern印跡雜交技術研究該基因的表達情況及該基因與腫瘤的關系。
Sequence data showed that the dna fragment containing a complete orf was 975bp , 68 . 3 % gc content . its amino acid product was a putative rna polymerase sigma factor , suggesting the orf was the true whig hologue 經測序和序列分析，證實該片段長975bp ， gc含量68 . 3 ，含有一個完整的orf ，編碼278氨基酸殘基，推測其蛋白產物是rna聚合酶因子。
Previously , we haved identified that ssmapkk was induced in stems by salt stress . then the ssvp orf was integrated into the plant expression vector pcambia1300 and ssmapkk orf was integrated into the plant expression vector prokii 以前的實驗已證明ssmapkk的轉錄水平受鹽脅迫的誘導，由此推測ssvp和ssmapkk都與鹽地堿蓬對鹽的反應有關。

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orf

orf n.【魚類】黑色金魚。

orfe