O - operon 中文

operon n.【生物學】操縱子。

n. 【生物學】操縱子。 “the operon“ 中文翻譯：第十章操縱子“ara operon“ 中文翻譯：阿拉伯糖操縱子; 阿糖操縱子“arabinose operon“ 中文翻譯：阿拉伯糖操縱子“gal operon“ 中文翻譯：半乳糖操縱子“galactose operon“ 中文翻譯：半乳糖操縱子“his operon“ 中文翻譯：組氨酸操縱子“hut operon“ 中文翻譯： hut操縱子“inducible operon“ 中文翻譯：誘導性操縱子“lac operon“ 中文翻譯： lac操作子; 乳糖操子; 乳糖操縱子; 乳糖操縱組“lactose operon“ 中文翻譯：乳糖操縱子“leucine operon“ 中文翻譯：亮氨酸操縱子“monocistronic operon“ 中文翻譯：單順反子操縱子“operon concept“ 中文翻譯：操縱子概念“operon gene“ 中文翻譯：操縱子基因“operon network“ 中文翻譯：操縱子網“operon theory“ 中文翻譯：操縱子說; 操縱子學說“polycistronic operon“ 中文翻譯：多順反子操縱子“repressible operon“ 中文翻譯：可阻遏操縱元; 阻遏操縱子,阻遏性操縱子“rrna operon“ 中文翻譯： rrna操縱子“trp operon“ 中文翻譯：色氨酸操縱子“tryptophan operon“ 中文翻譯：色氨酸操縱子“catabolite sensitive operon“ 中文翻譯：降解物敏感操縱子“dna lac operon“ 中文翻譯： dna乳糖操縱子“glucose sensitive operon“ 中文翻譯：糖敏操縱子,葡萄糖敏感型操縱子“opernhaus zürich“ 中文翻譯：蘇黎世歌劇院“opern“ 中文翻譯：歌劇

operose
Using pcr technology , a 2 . 4kb dna fragment , part of tryptopanase operon , containing a promoter , a regulator gene tnac located downstream of the promoter and a desired tryptopanase gene tnaa which can be expressed by the promoter from e . coli k - 12 , was cloned to pmd18 - t vector . the dna sequence is the same as which was published on science 為了證明質粒上的基因能表達出有活性的色氨酸酶，將這個dna片段克隆到pet28c質粒的bamhi和hind位點上，使該片段受t7rna聚合酶的啟動子控制，然后轉化噬菌體de3的溶源菌bl21 ( de3 ) 。
The c - phycocyanin ( cpc ) operon of blue - green alga ( or cyanobacteria ) arthrospira platensis fachb341 was cloned , sequenced and characterized by using chromoseme walking method . the sequence includes cpcb gene ( 519 bp ) , cpca gene ( 489 bp ) , cpch gene ( 357 bp ) , and upstream sequence of cpcb ( 427 bp ) and upstream sequence of cpch genes ( 184 bp ) , 111 bp of phycocyanin intergenetic spacer ( pc - igs ) . upstream sequence of cpcb gene was ligated into promoter - probe vector pegfp - 1 and transformed into three systems : e . coli , synechocystis pcc 6803 and a . platensis fachb341 by supersonic and electrophoresis methods 根據genbank中報道的節旋藻藻藍蛋白基因序列設計引物，首先克隆了鈍頂節旋藻( arthrospiraplatensisfachb341 )藻藍蛋白操縱子中亞基基因、亞基基因部分序列及二者之間的間隔區序列( pc - igs )并進行序列測定，然后根據此測序結果設計引物，通過染色體步移法克隆得到藻藍蛋白操縱子長度為2086bp的基因片段，其中包括藻藍蛋白亞基基因( cpcb ， 519bp ) ，亞基基因( cpca ， 489bp ) ，連接蛋白h基因( cpch ， 357bp ) ，亞基基因上游啟動子序列( 427bp )以及各基因之間的間隔區( pc - igs ， 111bp ； cpch與cpca間隔區， 184bp ) 。
Puc18 - 169 was a subcloned plasmid containing the whole operon sequence of l - hydantoinase from arthrobacter bt801 which can convert 5 - benzylhydantoin to l - phenylalanine . hydantoin hydrolase which is responsible for the ring opening of hydantoin is one of the components of hydantoin utility enzymes of arthrobacter bt801 . n - carbamoylase is a part of hydantoinase operon which can transform n - carbamoylamino acids into the corresponding amino acids 節桿菌( arthrobacter ) bt801是由軍事醫學科學院生物工程研究所保存的l -乙內酰脲酶產酶菌株， puc18 - 169是由本室構建的含有節桿菌bt801的l -乙內酰脲酶完整操縱子序列的亞克隆質粒。
Similarity coefficient and genetic distances between two arbitrary individuals were caculated according to the data of experiments , the pedigree was constructed by two means ( they were upgma and parsimony ) , and genetic diversity was discussedbased on selecting the best conditions of pcr with opg and opa primers from operon company , we obtained 163 bands using 24 random primers . in other words , 163 sites in crested ibis “ genome were detected . the bands from 23 primers showed polymorphism . minimurn of polymorphic ratio was 0 , maximum was 85 . 71 % , and average was 48 . 99 % . in the population which was composed by 37 individuals , most of genetic distances between two arbitrary individuals were less than 0 . 1 . the results showed that the degree of similarity among crested ibises was high while genetic diversity in the population was low accordingly 我們在對operon公司opg系列和opa系列引物進行優化篩選的基礎上，用24條隨機引物共擴增出163條譜帶，即檢測了朱?基因組中的163個位點，其中23條引物的擴增結果具有多態性；譜帶的多態比率最小為0 ，最大為85 . 71 ，平均為48 . 99 ；在由37只朱?組成的種群中，任意兩個個體之間的遺傳距離絕大多數在0 . 1以下。這些結果表明，目前朱?個體間的相似程度很高，種群的遺傳多樣性水平較低。
Second , genomic dna polymorphisms of three mandarinfishes populations ( each bulked dna pool composed by 10 individuals ) were tested by rondom amplified polymorphic dna ( rapd ) using bulked segregant analysis ( bsa ) . 12 primers selected from operon kitz yielded reproducible and polymorphic dna fragments ranging from 125bp to 3kb . these primers generated 60 scorable marker bands of which 15 ( 25 % ) were polymorphic in qiupu river population ; 59 bands in changjiang population , 15 ( 25 . 4 % ) were polymorphic ; and 58 bands in wanfuhu mandarinfishes , 19 ( 32 . 8 % ) were polymorphic 20個引物中有12個在三群體間產生多態性，這12條引物在秋浦河鱖魚中擴增出了60條帶，其中15條為多態性(占25 ) ；長江鱖魚59條帶， 15條為多態性(占25 . 4 ) ；萬佛湖鱖魚58條帶， 19條為多態性(占32 . 8 ) 。
21 wang l , trawick j d , yamamoto r , zamudio c . genome - wide operon prediction in staphylococcus aureus . nucleic acids res . , 2004 , 32 : 3689 - 3702 . 22 washio t , sasayama j , tomita m . analysis of complete genomes suggests that many prokaryotes do not rely on hairpin formation in transcription termination 本文所論述的不依賴終止子預測算法被整合于我們開發的rnall局部二級結構預測軟件包內,用戶可免費下載，網址為http : digbio . missouri . edu wanx rnall 。
2 . cloning of structural genes of bacillus subtilis bio operon diluted the genomic dna of bacillus subtilis as the template , long pcr product ( 10 . 3kb ) and three salvage pcr products were separately gained by optimization of reaction conditions of pcr 枯草桿菌生物素操縱子基因的克隆將枯草桿菌基因組dna稀釋后，通過pcr反應條件的優化，分別擴增得到了生物素操縱子基因的長距離pcr產物( 10 . 3kb )和3個分段pcr產物。
Bioinformatical analysis of pprl : pprl is the first orf in what appears to be a four - gene operon containing homologues of three genes ( folp , folb and folk ) in the folate biosynthetic pathway described in other prokaryotes Ppri的生物信息學分析： ppri是4個緊密相連基因的第一個基因，后面的三個分別是folp 、 folb 、 folk的同源物，它們參與葉酸的生物合成，因此ppri在基因組中的位置有點特殊。
For highly efficient expression of the structural genes of bio operon , a lot of researched materials were constructed which could commercially operated in this experiment and these materials provided some explored experience and reference materials for future research 并構建了可商業化操作的研究素材，為以后的研究提供探索性經驗。本論文主要作了以下幾方面的研究： 1
Contrasting analysis with the genes sequence of bacillus subtilis 168 strain ' s bio operon , the sequence of bio operon of as 1 . 1094 strain had 12 bases difference with that of 168 train , and 7 bases caused variation of amino acid 1094菌株與168菌株bioafdb基因序列完全一致， 9個差異堿基是由于pcr擴增導致的。枯草桿菌生物素操縱子基因4個亞克隆序列的測序結果證實， asi
However , in the late phase of the growth , the strain hv grew a little faster than e . colihms174 . hv ( ptz101 ) was constructed by the plasmid containing the phb operon and the parde gene tansformed into the strain hv 將帶有phb操縱子和質粒穩定分配基因parde的質粒ptz101引入vhb整合菌中，構建成為產phb的vhb整合菌hv ( ptz101 ) 。
Used the genomic dna extracted by low melting - point agarose embedding method as pcr template , the full length of structural genes of bacillus subtilis bio operon were gained by long pcr method 將該方法提取的基因組dna稀釋100倍作為模板，采用長距離pcr方法，獲得了枯草桿菌生物素操縱子基因全長。
This experiment probed how to improve the expression efficiency of the structural genes of bio operon , and provided the feasibly researched route and scientifically theoretical foundation 旨在探索如何提高生物素操縱子基因的表達效率，為該操縱子基因的高效表達提供可行的研究路線和理論依據。
Used as 1 . 1094 strain of bacillus subtilis as researched material , the structural genes of biotin operon ( bio operon ) were cloned , and its sequence were engineered 本研究以枯草桿菌asl . 1094菌株為研究材料，克隆了生物素操縱子基因，并對基因序列進行改造。
14 chen x , su z , dam p , palenik b , xu y , jiang t . operon prediction by comparative genomics : an application to the synechococcus sp . wh8102 genome 我們同時比較了采用單個兩個及三個參數的模型，發現采用三種參數的模型總體效率敏感率特異率最高。
Like eukaryotes , but unlike bacteria , they have introns in their transfer rna , but like bacteria they have polycistronic operons ( gene regulators ) 像真核生物而不像細菌，轉運rna中含有內含子，還有細菌不具備的多順反子的操縱子（基因的調控子）。
Operon is a unit of bacterial gene expression and regulation , including structural genes and cis - acting control elements in dna recognized by regulator gene product ( s ) 控制某一代謝途徑的相關基因,緊密連鎖地排列在一起,受同一操縱子控制。
Then the engineered genes of bio operon were carried on testing of the functional expression , and biow & biob gene were induced to express 而后對改造后的生物素操縱子基因進行了功能表達檢測及biow與biob基因的誘導表達。
Trp operon and attenuationtrp 操縱子和弱化

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operon

operon n.【生物學】操縱子。

operose

Used as 1 . 1094 strain of bacillus subtilis as researched material , the structural genes of biotin operon ( bio operon ) were cloned , and its sequence were engineered 本研究以枯草桿菌asl . 1094菌株為研究材料，克隆了生物素操縱子基因，并對基因序列進行改造。

14 chen x , su z , dam p , palenik b , xu y , jiang t . operon prediction by comparative genomics : an application to the synechococcus sp . wh8102 genome 我們同時比較了采用單個兩個及三個參數的模型，發現采用三種參數的模型總體效率敏感率特異率最高。

Like eukaryotes , but unlike bacteria , they have introns in their transfer rna , but like bacteria they have polycistronic operons ( gene regulators ) 像真核生物而不像細菌，轉運rna中含有內含子，還有細菌不具備的多順反子的操縱子（基因的調控子） 。

Operon is a unit of bacterial gene expression and regulation , including structural genes and cis - acting control elements in dna recognized by regulator gene product ( s ) 控制某一代謝途徑的相關基因,緊密連鎖地排列在一起,受同一操縱子控制。

Then the engineered genes of bio operon were carried on testing of the functional expression , and biow &amp; biob gene were induced to express 而后對改造后的生物素操縱子基因進行了功能表達檢測及biow與biob基因的誘導表達。

Trp operon and attenuationtrp 操縱子和弱化

Like eukaryotes , but unlike bacteria , they have introns in their transfer rna , but like bacteria they have polycistronic operons ( gene regulators ) 像真核生物而不像細菌，轉運rna中含有內含子，還有細菌不具備的多順反子的操縱子（基因的調控子）。

Then the engineered genes of bio operon were carried on testing of the functional expression , and biow & biob gene were induced to express 而后對改造后的生物素操縱子基因進行了功能表達檢測及biow與biob基因的誘導表達。