O - offish 中文 - EN166

offish adj.〔口語〕不親熱的，冷淡的，疏遠的；剛愎的。

offload
For the cryogenic preservation of fish , in this paper we made the primary culture of the kidney of allotetroploid crucian carp and primary studies were carried out on cryopreservation culture offish embryo cells derived from misgurnus auguillicaudatus or grass carp , the results of the experiments are as follows : the primary cell culture of the kidney tissue derived from allotetroploid crucian carp was carried out using tissue adherent culture , the primary observations of the growth conditions and morphology of the primary culture and subculture cells which originally come from the kidney tissue were also made 本文主要從魚類種質保存的目的出發，一方面以四倍體鯽鯉魚為材料，對四倍體鯽鯉魚腎臟組織進行初步培養，為建立相應細胞庫及下一步培養凍存的魚類胚胎細胞奠定基礎；另一方面，以魚類組織細胞培養技術為基礎，泥鰍胚胎細胞為材料，對魚類胚胎細胞凍存培養方法進行初步研究，并應用該技術方法對草魚早期胚胎細胞進行凍存培養實驗。報告如下：本文用組織貼壁法對四倍體鯽鯉魚腎組織進行原代、傳代培養。
The primary results showed : using m199 as diluents containing 20 % bovine serum , it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example , from 0 to - 6 freezing speed is about - 0 . 05 a minute , from - 6 to - 40 , freezing speed is about - 0 . 5 a minute ) , studies on effect of various concentration of dmso demonstrate that about 12 . 5 % dmso gave the highest post - thaw percentage of viable cells . the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus . the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula . the cells preserved in liquid nitrogen at - 196 were thawed and cultivated , a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 % . the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells 研究初步表明:以細胞培養液m199 (含2既的小牛血清，常規量雙抗)為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜采取先慢后快的方式(例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ) ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天后均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
Making anti - dis ease breeding researches of fish by transfer gene is a new direction in transfergene researches offish 通過基因轉移進行魚類抗病育種研究，是魚類轉基因研究中的一個新的方向。
She used to be aloof and stand - offish with us , but we are now on first - name terms 她一向對我們疏遠冷淡，但現在我們成了很好的朋友了。
Brenda is a very friendly girl but her parents are a bit stand - offish 布倫達是一個非常友好的姑娘，可是她父母親有點冷漠。
Southern jiangsu is a famous land offish and rice in chinese history 蘇南地區是我國歷史上著名的魚米之鄉。
He ' s not like geoffrey , he seems stand - offish 他不像格雷戈里,他對我很不友好
She's not fond of himquite offish and quite careless . 她并不喜歡他對他相當冷淡，完全不掛在心上。