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napu

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napu n.【動物；動物學】(爪哇、蘇門答臘產之)矮麝香鹿。

n. 【動物；動物學】(爪哇、蘇門答臘產之)矮麝香鹿。 n. 〔美俚〕＝napoo. “napua“ 中文翻譯：   納普阿山“napton“ 中文翻譯：   納普頓“napuka atoll“ 中文翻譯：   納普卡環礁“napthenic oils“ 中文翻譯：   環烷油“napuka island xx“ 中文翻譯：   那普卡島“napthalene powder“ 中文翻譯：   萘粉“napumbalu pulau“ 中文翻譯：   納蓬巴盧島“napthalene flakes(refined)“ 中文翻譯：   精萘“napumpum“ 中文翻譯：   納蓬蓬“napthalene flakes(industrial grade)“ 中文翻譯：   工業萘

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“ xiyuan4 “ , a b . olereacea l . and “ 2001817 “ , a b . napus l . were bred by southwest agricultural university . here using them as materials , we studied and explored thoroughly the dna and cdna sequences of thl2 gene and mod gene with bioinformatical methods . for the first time , based on sequence data , we analyzed the structure , heredity and variation of the dna sequences encoding thl2 and mod , and foresaw the molecular mechanism in self - incompatibility signal process 本研究以西南農業大學繁育的高度自交不親和的‘西園4號’甘藍和‘ 2001817 ’油菜為試材，采用生物信息學的方法全面研究和探索性的分析了thl2基因和mod基因的dna與cdna序列，首次從序列數據出發研究這兩個編碼基因的結構與遺傳變異，以及在自交不親和性信號轉導中的作用機理

The objectives of this study are to clone the ban fragment of brassica napus and to apply the floral - dip in the transformation of oilseed rape to establish a convenient method for oilseed rape breeding . ban is dfr - like gene in chalcone synthesis pathway functioned as a negative regulator , and has been well studied in arabidopsis thaliana 為利用轉基因技術創造甘藍型黃籽油菜，本文克隆了與甘藍型油菜( brassicanapus )色素生物合成有關的ban基因同源片段，并探索了甘藍型油菜花序浸泡法轉基因新方法。

The method of ctab was specifically improved for the extraction of genomic dna and total rnas from brassica oleracea l . and brassica napus l . 2 . arclboler gene was amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) 以甘藍( 200110197 )和油菜( y578 - 2 )自交不親和系的植株為材料，提取幼苗中的gdna及花蕾期的柱頭、子房、花瓣、葉片、根的mrna ，并對mrna進行反轉錄合成cdna ，然后分別以gdna與cdna為模板擴增arc1蛋白編碼序列。

Thl2 gene was finally amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) . the dna sequencing result shows that the genomic gene of thl2 is approximately 900 bp from brassica oleracea l . in contrast to that of 850 bp from brassica napus l . 采用阿r和rtpcr技術，從甘藍和油菜基因組和柱頭總則a中擴增獲得了thlz基因，基因組中得到的基因大約為900hp和850hp ， cdna大約為477hp 。在thlz基因中首次發現了兩個內含于，并且甘藍和油菜thlz基因的內含于序列同源性低于ich ，并且大小也有差異。

Two primers , designed according to the conserved regions of ban gene in arabidopsis thaliana , were used to amplify the ban homologous fragments from the genomic dna of brassica napus , b . chinensisl , b . juncea , a . thaliana and other cultural plants of cruciferae . the very similar pcr fragments were obtained from all the amplifications , which implicated that ban may be a conserved gene existing widely in the genomes of cruciferae . pcr fragments were cloned and confirmed by restriction enzyme digestion 參照擬南芥( arabidopsisthaliana ) ban基因與cdna設計引物，對甘藍型、白菜型、芥菜型油菜，擬南芥及其它十字花科栽培品種的基因組dna進行pcr擴增，均擴增出與擬南芥ban基因擴增片段大小極其相似的dna片段，提示ban可能廣泛存在于十字花科植物中。

2 . cloning and sequence analysis of mod gene using the genomic dna and pistil total rna isolated from b . olereacea l . and b . napus l . as templates in pcr and rt - pcr reaction , the mod dna and cdna were amplified , recovered , cloned into vectors and sequenced Mod基因的克隆與序列分析以甘藍和油菜的基因組dna和柱頭總rna為模板，采用pcr和rt - pcr技術擴增moddna和cdna ，電泳后對目的帶進行回收、禿隆、兩端驗證，測序獲得其dna和cdna序列。

The start codon is at 84 - 86bp , the stop codon at 1658 - 1661bp . the homologous comparison of the deduced amino acid sequence with other plant epsp synthase proteins shows the identity to those of b . napus , a . thaliana , tomato , n . labacum respectively are 92 % , 91 % , 80 % , 65 % 同源比較該段序列所編碼的氨基酸序列可以發現epsp合成酶有較高的保守性，它與歐洲油菜、擬南芥、西紅柿、煙草的同源性分別為920 、 91o 、 80o 、 65o 。

Homologous analysis in amino acid sequence showed that between thl2 in b . olereacea l . and trx4 in arabidopsis thaliana were 97 % in identity , a little difference to that of 86 % in identity between thl2 in b . napus l . and trx4 in a . thaliana 氨基酸水平上的同源性分析表明甘藍thl2與油菜thl2和擬南芥中硫氧還蛋白trx4同源性較高。分別達到97和86 ，進化分析也表明thl2和trx4具有較高的親緣關系。

Cloning and sequence analysis of thl2 gene using the genomic dna and stigma total rna isolated from b . olereacea l . and b . napus l . as templates in pcr and rt - pcr reaction , the thl2 dna and cdna were amplified , recovered , cloned into vectors and sequenced Thl2基因的克隆與序列分析提取甘藍和油菜的基因組dna和柱頭總rna ，采用pcr和rt - pcr技術擴增thl2dna和cdna ，對目的帶進行回收，克隆并測序。

Abstract : the results indicate that yellow seed variety of b . napus rape is as a mixture of various yellow seeds , there are significant differences among the lines tested and different segregation of individual plants each other in its penetrance of the seedcoat 文摘:研究了甘藍型黃籽油菜不同品系材料、不同單株間千粒黃籽外顯率及其與含油量、黃籽級數和千粒重的關系。

The results indicate that yellow seed variety of b . napus rape is as a mixture of various yellow seeds , there are significant differences among the lines tested and different segregation of individual plants each other in its penetrance of the seedcoat 研究了甘藍型黃籽油菜不同品系材料、不同單株間千粒黃籽外顯率及其與含油量、黃籽級數和千粒重的關系。

3 . the mechanism of dna integration mediated by homologous recombination in chloroplast transformation was tentatively explored . we isolated two chloroplast dna fragments from brassica napus and used them as targeting sequence to integr 成功地通過h次轉化獲得整合并表達多基因的轉基因煙草，縮短了研究周期，對相關轉基因植物的研究有一定參考價值。

The cdna of thl2 from brassica napus l . is 447 bp . thl2 gene contains two introns with wide divergences between brassica oleracea l . and brassica napus l . . however , the deduced amino acid sequence of thl2 protein from brassi 5ug的總rna為模板，進行定量rt pcr ，結果分析表明am基因在甘藍中的表達特異性和油菜一樣，均是在花柱柱頭表達的。

With 5 male sterile lines in brassica napus l . as materials , the development of buds and stamens and pistils of male sterile lines in b . napus l . were researched and maked a comparison with the control group 摘要以5種不同類型甘藍型油菜雄性不育系為材料，以保持系作對照，對甘藍型油菜雄性不育系的花蕾及雌雄蕊干物質變化動態進行了研究。

In order to avoid promoter - homology - mediated gene silencing , we isolated another seed - specific promoter nap300 from brassica napus h165 to replace 75 promoter in constructed expression cassettes 將nap300與gus基因相連進行功能鑒定。熒光檢測和組織化學染色的結果都證明此僅300bp的dna序列足以調控基因進行種子特異性表達。

Thl1 gene was also amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) 采用pcr和rt - pcr技術，從甘藍和油菜基因組dna和柱頭總rna中擴增獲得了719bp的thl1基因和430bp的cdna序列。

Comparing the mod cdna of b . olereacea l . with that of b . napus l . , 31different bases were found , and only one of them led to the alteration of amino acid residues based on the predicted amino acid sequences 對甘藍和油菜的五少口刀cdna序列進行比較發現，它們之間有31處堿基差異，只有1處造成了氨基酸的變異。

Different waterlogging tolerances of nine rapeseed ( brassica napus l . ) genotypes were investigated after germinating seed anoxic stress for 12h and spring field waterlogging for l0d 摘要9個不同遺傳背景的甘藍型油菜品種（系）在室內進行發芽種子水淹和春季田間模擬濕害條件下，研究油菜對濕害脅迫的耐濕遺傳差異。

There was a significant negative correlation between electric conductivity and vigor index , indicated the electricl conductivity could be used as a selective index for flood tolerance breeding in brassica napus l 電導率與活力指數之間有極顯著的負相關，可用來快速篩選油菜耐濕性材料。