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The regeneration system of tobacco was established . the adventitious shoots were induced from leaf explants of tobacco based on ms basal medium supple - - mented with 2 . 0mg / l 6 - ba and 0 . 3mg / l naa . then the regenerated plants were rooted on ms medium containing 0 . 3mg / l naa 植株再生體系的建立采用無菌苗煙草葉片為外植體,以ms為基本培養基,篩選出ms + 6 - ba2 . 0mg l + naa0 . 3mg l以誘導不定芽的分化,并于ms + naa0 . 3mg l的pa碩士學位論文v沉了砂“工隊nk ”主根培養基上生根,獲得完整再生植株。

No callus occurred from hairy roots on medium lacking 2 , 4 - d . high concentration ( l . 0mg / 1 ) of 2 , 4 - d could induce much more calli , but it obstructed the growth of calli and followed buds differentiation . the result of callus induction showed that the optimum medium for callus induction was ms + 0 . 5mg / l 2 , 4 - d + 0 . 5mg / l 6 - ba and ms + 0 愈傷組織誘導最佳培養基為: ms 05mg l個d 05mg l ba ,愈傷組織生長的最佳培養基為ms 0ling 12 , 4 d cling 16ea ;愈傷組織能在不含激素的培養基上自發地分化出不定芽,但分化頻率較低,而6ea對不定芽的分化作用較明顯,配合使用6習a和naa對芽的誘導及得芽率較好,不定芽誘導的最佳培養基為ms 10mgilnaa 05mg lia培養基。

It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions , clearing aged cells and metabolic products , regulating immune responses and protecting against infection . in some pathological states such as psoriasis and contact dermatitis , a certain serum level of the antibody could inhibit the progression of the diseases , and is beneficial to the recovery from the diseases . after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies , meanwhile , experiencing an intensive academic debating on whether naas a “ horror autotoxicus “ or a “ gnothi seaution ( know yourself ) “ , a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability , immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis , and recombinant antibody is under investigating 抗角蛋白自身抗體( akautoab )是naa的重要組成部分,以往實驗通過雜交瘤技術、免疫親和層析技術和噬菌體抗體庫技術分別獲得單克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ,并對akautoab免疫學特性及在體生理和病理意義進行了廣泛的研究,直接或間接地發現akautoab是機體正常免疫調節網絡的組成部分,在維護某些生理狀態的穩定、清理衰老細胞及代謝產物、調節免疫和抗感染等方面起到重要作用;在某些病理情況下(如銀屑病、接觸性皮炎等) ,體內akautoab的組分和滴度會發生變化,而正常水平的akautoab則有利于限制病情的發展,促進損傷的修復。

Thus , it will help elucidate the development , self tolerance of self autoreactive b lymphocyte repetoire to investigate the generation of naa . researches on the function of naa will help clarify the significance of naa ' s existence . the exploration of biological nature , encoded gene and the difference or relation between naa and pathological autoantibody will shed much light on the pathological mechanism and treament of autoimmune disease 因此,對naa產生機理的揭示將有助于回答免疫學b細胞產生、發育以及自身耐受的問題;對naa功能的研究有助于進一步明確naa對機體的意義;對naa的特性、編碼基因、與病理性自身抗體界限和關系的研究可能對自身第四軍醫大學碩士學位論文免疫疾病的發病機制和治療提供有益的線索。

S . regeneration system of mustard hypocotyls and cotyledons of 4 days and leaves of 25 days were used as transformation materials , and ms as basic medium supplying different ba ^ naa and kt concentration , we got the optimum explant adventitious bud inducing medium ( ms + 6 - ba3 . 0mg / l + naa0 . 2 mg / l ) . among the three kinds of mustard explants . the cotyledons showed the highest adventitious bud regeneration capacity . and the regeneration capacity of leaf explantswas weaker . and the weakest of hypocotyls 芥菜高頻再生體系的建立以4天左右的葉用和莖用芥菜子葉、下胚軸和25天苗齡的芥菜無菌苗的葉片為外植體,以ms為基本培養基,通過加入不同濃度和不同組合的ba 、 naa 、 kt ,篩選到了外植體不定芽分化的最適培養基( ms + ba3 . 0mg / l + naa0 . 1mg / l )及生根的最適培養基( ms + naa0 . 2mg / l ) 。

Bap performed more important function than kt in differentiation of tall fescue embryogenic calli , but better results could be achieved with combination of 2mg / l bap and 0 . 5mg / l kt . at this cytokinin level , 0 . 5mg / l naa was recommended to obtain the highest callus regeneration frequency . plant regeneration could be evidently boosted when embryogenic calli were pre - differentiated on high - osmoticum medium with 60g / l sucrose , and / or when the pre - differentiated compact calli were differentiated on differentiation medium solidified with l0g / l agar 高羊茅胚性愈傷組織分化時, bap的作用要比kt大,但2mg lbap與0 . 5mg lkt配合可獲得更佳的效果;在該細胞分裂素水平下,生長素naa用0 . 5mg l ,愈傷組織再生率最高;胚性愈傷組織先在含60g l蔗糖的分化培養基上高滲預分化,以及經高滲預分化后的致密愈傷組織在瓊脂濃度為10g l的分化培養基上分化,能明顯促進愈傷組織的植株再生;在分化培養基中添加脯氨酸導致愈傷組織再生率下降,但同時有減少白化苗再生的趨勢。

But cluster buds can not be obtained from the callus of stems and embryo . it has likely relate to the inner structure of callus . lt is very difficult to get embryoid from mature embryo and its inducing rate is very low , and only 11 . 5 % in the media containing n6 + ba 1 . 5mg / l + naa 0 . 5mg / l + sucrose 30g / l + agar 8g / l + ac 2g / l which is the best media for gingo tissue culture 而莖段和胚誘導的愈傷組織基本上沒有叢生芽發生,這可能和不同愈傷組織的內部結構有關。成熟胚的胚狀體誘導率較低,誘導相當困難,在n6 bai

( 3 ) regenerating plantlets were obtained via somatic embryo , which derived from hairy root . the effects on the induction of somatic embryos from hairy root were investigated using orthogonal test . the best somatic embryo induction was obtained on ms + 0 . 5mg / l naa + 0 . 5mg / l kt + 30g / l sucrose 單獨使用naa很難誘導出體胚,單獨使用kt或kt與naa配合使用均能誘導出體胚, naa 、 kt和蔗糖對體胚誘導的影響作用依次是t a蔗糖。

Calli were induced by culturing hypocotyls of pepper in the ms medium supplemented with 2 . 0mg / l 6 - ba and l . omg / l naa . the diploid wound callus was treated by adding colchicines into the medium and by immersing into colchicines solution for different concentrations and periods 通過將不同濃度的秋水仙堿加入培養基和不同濃度的秋水仙堿溶液浸泡兩種方法處理愈傷組織,從而篩選出最佳的誘導方法和最佳的秋水仙堿濃度和處理時間。

Is one of the most valuable and most widely cultivated small fruits in the world . while the global soil salt stress and drought caused more and more serious affection on strawberry production . it is necessary to culture salt and drought resistant strawberry cultivars via foreign gene transformation 以不同品種草莓(達賽和豐香)花藥為外植體,在附加6 - ba與2 , 4 - d 、 6 - ba與naa的ms培養基上進行培養,均可誘導出愈傷組織。

This paper deals mainly with the extraction of geological and hydrogeological information from deep strata through the comparative analysis of major elements , minor elements and trace elements in rocks and in relics of dried fountains by means of neutron activation analysis ( naa ) 摘要利用中子活化測試結果,對巖石及泉水乾涸殘馀物中常量及微量元素的進行分析對比,以?解深層巖體有關的地質與水文地質信息。

Anti - keratin auto antibody ( ak auto ab ) is accepted generally as a significant component of natural auto antibody ( naa ) which is found in human as well as vertebrate , and is of characteristic of polyreactivity , low avidity , extensive connectivity and shorting of specificity to the species 天然自身抗體( naa )廣泛存在于人和脊椎動物體內,具有多反應性、親和力低、締和性廣及種屬特異性差的特點。

Cotyledon and true leaves of stem mustard were cultured on ms medium supplemented with different concentrations of various cytokinins ( ba , cppu , tdz or kt ) alone or in combination with naa or iba , and the adventitious buds regeneration rate was evaluated . 以榨菜子葉、真葉為外植體進行離體培養,用ba 、 cppu 、 tdz和kt等細胞分裂素和生長素naa 、 iba組合誘導子葉、真葉再生不定芽試驗。

As explants , were infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring the expression vectors pbgsb and pbglsb respectively and co - cultured for 3 days in darkness on the culture medium ( ms + 0 . 1 mg / l 6 - ba + o . l mg / l naa + 0 . 7 % agor ph 5 . 7 ) Ling inaa )中暗培養三天后,移入加入抗生素100mg lkan和500mg幾cr的誘芽培養基,于組培室中進行誘芽和抗性篩選。

Among the three factors tested in our experiments , kt had the significant effect on the somatic embryo induction and its development , followed by naa sucrose had smallest effect on somatic embryo induction , but had the obvious effect T 、 a濃度和蔗糖對體胚的發育均有較明顯的作用。對體胚的發育最好在cling l的kt濃度下或不含激素的培養基中,蔗糖濃度最好在30g l 。

Naa can not only defend the invasion of various microorganism , but maintain the stability of internal envioment , lead to self tolerance , regulate immunity and prevent tumor progression 從不同角度對naa的研究顯示: naa的產生可能與自身反應性b細胞克隆陽性選擇過程有關; naa對外可以抵御外來微生物的入侵;對內可以維持機體內環境的穩定,引起自身耐受,調節免疫和抗腫瘤。

High frequency of bud induction was found on ms medium supplemented with 6 - ba the optimum medium for adventitious bud induction was ms + l . 0mg / 1 naa + 0 . 5mg / l 6 - ba , and ms + 1 . 5mg / l naa + 0 . 5mg / l iaa for bud rooting 門)誘導毛狀根體細胞胚再生植株。通過正交實驗篩選出了毛狀根體細胞胚誘導的最佳培養基為: ms 05mg lnaa 0 sing kt 30gil蔗糖。

The enriched stable isotope tracer technique combined with neutron activation analysis and differential centrifugation was utilized to investigate the subcellular distribution of rare earth elements sm and yb in rat liver Sm和, sup 168 yb作為示蹤劑,結合差速離心分離和中子活化分析技術naa初步研究了稀土元素sm和yb在大鼠肝臟亞細胞組分中的分布。

It is easy to induce callus derived from hypocotyls and cotyledons , but callus derived from root tip was rather difficult to be done . the most suitable medium for callus induction was the ms medium containing ba 0 . 5 mg / l and l . omg / l naa 愈傷組織的誘導與再分化的研究顯示: “種都一號”兒菜下胚軸與子葉愈傷組織誘導較容易,但根尖很難愈傷化。