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mutagenesis

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mutagenesis n.突變。

n. 突變。 “acridine mutagenesis“ 中文翻譯：   吖啶誘變“bacterial mutagenesis“ 中文翻譯：   細菌誘發發生“casette mutagenesis“ 中文翻譯：   盒式誘變“cassette mutagenesis“ 中文翻譯：   盒式誘變“chemical mutagenesis“ 中文翻譯：   化學突變誘發; 化學物誘變作用; 化學誘變; 化學致突變作用“combinative mutagenesis“ 中文翻譯：   組合突變形成“combinatorial mutagenesis“ 中文翻譯：   組合突變“deletion mutagenesis“ 中文翻譯：   缺失定位; 缺失誘變; 缺失作圖“directed mutagenesis“ 中文翻譯：   定向突變; 定向誘變“environment mutagenesis“ 中文翻譯：   環境誘變“environmental mutagenesis“ 中文翻譯：   環境誘變“exhaustive mutagenesis“ 中文翻譯：   徹底誘變,全面誘變,飽和誘變“invitro mutagenesis“ 中文翻譯：   體外誘變“misrepair mutagenesis“ 中文翻譯：   誤修復誘變“mutagenesis in vitro“ 中文翻譯：   突變發生“mutagenesis in vivo“ 中文翻譯：   體內誘變“radiation mutagenesis“ 中文翻譯：   輻射誘發突變“random mutagenesis“ 中文翻譯：   無規突變誘變“saturation mutagenesis“ 中文翻譯：   飽和誘變[對基因的某一小區域內堿基進行多種形式的置換“silent mutagenesis“ 中文翻譯：   沉默誘變“ty mutagenesis“ 中文翻譯：   ty誘變,酵母轉座子誘變; 酵母轉座子誘變“ultraviolet mutagenesis“ 中文翻譯：   紫外誘變“untargetted mutagenesis“ 中文翻譯：   非目標誘變“alkylating agents mutagenesis“ 中文翻譯：   烷化劑誘變“mutagene specificity“ 中文翻譯：   誘變劑特異性“mutagen specificity“ 中文翻譯：   誘變原特異性

mutant

At last , three strains of high activity was induced mutagenesis by ntg , after the routine screening procedures , activity of n28 and n118 was higher 47 . 5 and 40 . 9 percent than that of a . niger an01001 , and higher 29 . 5 and 23 . 7 percent than that of a199 最后，用亞硝基胍對產酶較高的3個菌株誘變，經復篩后， n28和n118的酶活比黑曲霉an01001高出47 . 5和40 . 9 ，比a199高出29 . 5和23 . 7 ， n28和n118經過5次連續傳代后，產酶能力基本穩定。

Dominant negative alleles of gpil7p were produced by random pcr mutagenesis of three conserved fragments of gpi17p : 1 ) having generated the plasmid library which contains 100000 plasmids harboring gpi17 mutants then screened from 8000 clones , we got 25 strong and 3 very strong dominant negative alleles 對gpi17p可能的三個保守區進行了pcr隨機誘變，并最終獲得顯性負性等位基因： 1 )組建了gpi17p突變體質粒庫，其所含質粒數約為100000 。

Protoplast regeneration , treatment of mutagenesis and other unknown factors can increase the genomic inherent instability , which usually affects the following five phenotypic traits : sporulation , pigmentation , colony morphology , antibiotic production and antibiotic resistance 基因組的不穩定性通常會影響菌株的以下五種表型特征：產孢，產素，菌落形態，抗生素產生水平以及對相應抗生素的抗性水平。

Abstract : the studies on biological effects of rare earth have been summarized , including toxicity , distribution and transportation in vivo , carcinogensis , mutagenesis , teratogenesis to human or animals , influcnces on some proteins , enzymes , dna , rna 文摘:本文綜述了稀土的生物效應，包括稀土的毒性，稀土在機體內的分布、運移，稀土對人或動物的致癌、致畸、致突以及稀土元素對蛋白質、酶的作用及影響等。

This paper analysised the salient features of space mutation technique , discussed the mechanism of plant space mutagenesis and reviewed the research and application of space mutation breeding in germplasm resources innovation of landscape plants 摘要本文從植物空間育種技術特點分析入手，綜述了其誘變機理的研究進展，并對其在園林植物種植資源創新中的研究與應用進行了簡要的回顧與展望。

Furthermore , we found the mutagenesis relies on the alteration on gene expression profile induced by mnng treatment ; and also , the expression of dna polymerase p ( pol p ) was proved increased after mnng treatment 這種不依賴于dna損傷的非定標性突變隨后被證明依賴于mn ’ ng引起的細胞內基因表達的變化。進一步我們用rtpcr技術證明dna聚合酶e （ p 。 lp ）的表達在mn ’ ng處理后顯著升高。

Therefore , it is important to study the profiles of gene expression , which will help understand the global cellular stress responses to chemical carcinogens , and further elucidate the mechanisms of nontargeted mutagenesis 所以，從整體上研究烷化劑作用后細胞基因表達譜改變，對于了解化學致癌物誘發的哺乳動物細胞應激反應的全貌和揭示非定標性突變的發生機制具有非常重要的意義。

Thus , we hypothesized that pol p might be involved in mnng induced untargeted mutagenesis . establishment of pol p down - regulated cell line : to verify the hypothesis , a human pol p down - regulated cell line was established using an antisense rna strategy Lp表達阻斷的細胞系：為驗證此假設，我們用反義na技術3一i浙江大學博士學位論文王谷亮1建立了pcid表達下調的轉基因細胞系。

The experimental procedure that begins with a cloned segment of dna , or a protein sequence , and uses this knowledge to introduce programmed mutations ( through directed mutagenesis ) back into the genome in order to investigate gene and protein function 在已知dna克隆片段或蛋白質序列上引導程序性的變異（通過直接誘變）并返回到基因組，來研究基因和蛋白質功能的實驗方法。

Resveratrol , as secondary metabolite of grape fruits , has the functions of oxidation resistance , heart disease prevention , cancer prevention , blood vessel relaxation , reducing blood fat , platelet aggregation resistance , and mutagenesis resistance etc 摘要白藜蘆醇是葡萄中的次生代謝物，具有抗氧化、預防心臟病和防癌、防血管松弛和降血脂、抗血小板凝集和抗誘變等作用。

Direct damage on supf trna gene can be neglected because half - life of mnng is 1 . 1 hour and the interval between treatment and transfection was as long as 12 - 24 hours . therefore the mutagenesis is not lesion directed 20m的mnng經洗滌后的極微量殘留經過10 ? 20多個半衰期的降解后，己不足攻擊轉人的qdna分子，因此這種突變顯然是發生在mnng直接攻擊部位以外的正常堿基上。

A mutant library of xanthomonas campestris pv . campestris ( hereafter xcc ) strain 8004 with 17820 clones was constructed by random transposon tn5gwsa5 mutagenesis , which cover 1800 predicted orfs of xcc genome 用轉座子tn5gusa5誘變野油菜黃單胞菌( xanthomonascampestrispv . campestris以下簡稱xcc ) ，構建了xcc8004tn5gusa5插入突變體庫。

This paper introduces the principles and characteristics of magnaporthe grisea mutagenesis induced by physical and chemical inductions and genetic transformation briefly and reviews the research advances of these techniques 摘要系統地介紹了稻瘟病菌物理誘變、化學誘變和遺傳介導突變的原理、特點和研究進展，并評述了它們各自的優缺點。

Solving multiple - instance and multiple - part learning problems with decision trees and decision rules . application to the mutagenesis problem . lecture notes in artificial intelligence 2056 , stroulia e , matwin s eds . , 由于本文已經揭示出多示例學習與監督學習之間具有密切聯系，因此本文提出通過建立多示例集成來求解多示例學習問題。

However , organism can react to the stress actively and the mutagenesis is not a passive event . sos response is employed in dna damaging agents challenged e . co / i , to cope with abnormal conditions 當受到外源dna損傷因子的攻擊時，大腸桿菌e . coil能啟動sos反應以應對不良環境，提高對致損傷刺激的耐受能力，減少損傷的發生。

The use of retrovirus - mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes required for embryonic development and cell growth 運用反轉錄病毒介雜的插入突變技術，在脊椎動物斑馬魚中已經獲得了許多影響胚胎發育和細胞生長過程的突變體，并找到了對應的基因。

It was suggested that exogenous dna fragments ( mainly non - coding sequence ) had new mutagenetic mechanism as well as insertional mutagenesis . in t1 and t2 , a number of mutants were obtained 對這種現象的分析表明，外源全dna片段(主要是非編碼序列)對轉化受體除了插入誘變的作用外，還可能存在一種新的誘變機制。

Studies suggest that it probably interacts directly with the viral polymerase , and we want to confirm that by mutagenesis and by enzymatic activity assays , “ said tao 有研究表明，核蛋白可能會和病毒的聚合酶直接發生相互作用，我們將通過人工誘變技術及酶活性測定等技術來核實這一說法。 ”

Insertional mutagenesis using t - dna , transposons and plasmids axe commonly methods to creating a mutant library . here we summarize these strategies as well as the progress in the functional genomes research 本文分別介紹三種方法的原理及其在功能基因組學研究中的應用和研究進展。