M - mut 中文

mut n.＝ mutt.

mutability
The hwtx - i gene was chemically synthesized according to its known cdna sequence , the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor , the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis , then it was transformed into host strain gs115 , a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations , the multicopy transformant was named gh1 . gh1 was cultivated in flasks . after 6 days of induction by 0 . 5 % methanol , the supernatant was checked by 16 . 5 % tricine - sds page , which showed there was a band in the position of 3 . 5 - 6 . 1kd , then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column , after reverse phase hplc of ci8 and vacuum drying , the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page , maldi - tof mass spectrometry , amino acid composition analysis , the n - terminal amino acid sequence and its biological activity , the final field of the purified rhwtx - i was about 80mg / l , accounting for 23 . 6 % of it total secretory proteins 將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆并經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的( nh _ 4 ) _ 2so _ 4沉淀， yw - 3 ( mwc03000 )的超濾膜超濾，再經cm陽離子交換， c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
Sds - page results showed that there was a clear target protein band in mut + recombinant supernatant after 48 hours of culturing , while a faint band only in muts recombinant after 72 hours . western - blotting result showed that there was no remarkable difference of yield between mut + and muts recombinants after 6 days induced . anti - virus activity tests revealed that culture supernatants of mut + and muts recombinants could inhibit tmv infection with high efficiency in the same concentration and there was no significant difference between them 結果表明，誘導培養48小時后， mut ~ +重組菌株表達產物在sds - page膠上顯現出清晰的目的蛋白帶，而mut ~ s重組菌株培養72小時才能顯示微弱的目的帶； western - blotting雜交信號強度表明，同樣培養6天的mut ~ +和mut ~ s重組菌株表達產物在表達量上沒有明顯差別。
Sds - page results showed that as to mut + recombinant highest yield was obtained after 4 days inducing and with the culture time prolonged it reduced . pokeweed antiviral protein gene expressed well when methanol concentration reached 10g / l . pokeweed antiviral protein obtained high yield in thin acidic culture medium ( ph6 . 0 - 6 . 4 ) and its quantity in total mass of secrete protein exceeded 30 % Sds - page分析結果表明， mut ~ +組菌株在甲醇誘導第四天后pap在培養液中積累量達到最高水平，延長培養時間會導致產量下降；在10g / l的甲醇濃度誘導下， pap的表達量達到最高；培養基ph值在偏酸性條件下( 6 . 0 - 6 . 4 ) pap的表達量都維持在較高的水平。
The positive transformants with the integrates mn - sod gene was identified by zeocin - resistance , pcr screening and expression in p . pastoris . the recombinant mn - sod protein was successfully expressed in pichia pastoris based on the evidences that a relative molecular weight about 23kd appeard in sds - page , the obvious activity of sod existed in native - page and enzymatic activity test , and mn - sod activity was specific base on the inhibition with the mixture of chloroform - enthanol ( 3 : 5 / v : v ) and potassium cyanide . two secreted plasmids ppiczaa - sodm18 and ppiczaa - sodc were constructed and after there linearization were transferred into chromosome of pichia pastoris gs115 by electroporation Pcr鑒定及mut表型分析進一步說明，目標基因已經重組到宿主菌基因組染色體上； 0 . 5甲醇誘導表達后， sds - page結果顯示，表達的蛋白相對分子量約為23kd ，活性電泳出現明顯活性條帶；酶活性測定顯示，重組菌株sod活性比對照提高5倍左右；氯仿-乙醇( 3 : 5 v : v )和kcn ( 5mmol l )抑制反應進一步證明，所表達的sod為錳超氧化物歧化酶。
The objective of this research is to transform the cloned phytase gene into pichia pastoris in order to obtain high - yield phytase - producing strain and to optimize the engineered yeast media recipes for the scale - up production of phytase . main results of this research are as follow : 1 , xba i - linized recombinant plasmid ppic9k - phya was transformed into pichia pastoris by gene pulser . 98 positive transformants showing measurable phytase activities were screened on md plates and ypd plates containing g418 . they all grew quickly on both md plates and mm plates , which proved their phenotypes of methanol utilization were mut + 主要實驗結查如下：西南農業大學碩士學位論文1 、用乃ai酶切攜帶植酸酶基因表達片段的重組質粒ppicgk夕句) a ，回收dna ，用genepulser電擊轉化畢赤酵母，涂布md平板，又用含不同濃度g418的ypd平板進行抗性篩選，得到98個可檢測到植酸酶活力的陽性轉化子，它們在md 、 mm平板上均表現快速生長，說明其甲醇利用表型是mut干。
Subsequent to the forum , the association has consolidated the views and prepared “ the other hong kong report . “ moreover , our chairlady , ms lam yu - kiu , and our executive committee member , mr lee kar - mut , will represent the association to participate in the ngo forum of the second world assembly on ageing in spain to present this report . the final reprot will be compiled after the world assembly 本會隨后整理大會意見編印成香港民間報告，委派主席藍宇喬女士及執行委員李迦密先生代表出席第二屆聯合國國際老年大會非政府機構會議，爭取于大會上發表上述報告。在國際老年大會完結后，本會將依據聯合國會議結論及建議，再修訂報告，正式出版香港民間報告，并倡導長者團體及政府就未來的安老工作方向研究及討論。
Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115 . mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting 本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。
The yield reached 10 - 12mg / l . dr . chen ding hu in plant virus group of institute of plant protection of the caas ( chinese academy of agriculture sciences ) selected pap muts recombinants and wanted to produce pap through fermentation to prevention and cure plant virus disease 中國農科院植保所植物病毒實驗室陳定虎博士成功的篩選到p . pastorispap利用甲醇緩慢型重組子( mut ~ s ) ，希望能利用微生物發酵的方法來大量生產pap ，將其應用于植物病毒病的防治。
We derive and prove some system reliability indexes such as reliability , instantaneous availability , steady - state availability , mut , mdt and so on for these three new models . some numerical examples are given to illustrate the results in the thesis 本文對所建立的三個新模型的一些可靠性指標,其中包括瞬時可用度、穩態可用度、平均停工時間、平均開工時間、忙時等,進行了推導與證明,并對結果進行了分析與數值演示。
We also constructed ppiczaa - a - 1bgp vector , and transformed it into gs115 , then confirmed the mut phenotype , examined the presence of insert using pcr , conducted small - scale expression , analyzed the expression by sds - page and western blot 另構建了ppiczaa a叫酵母表達載體，轉化gslls菌株，確定表型， pcr篩選a ibgp有效整合酵母基因組的菌株，進行誘導表達， sds一page凝膠電泳和westernblot鑒定表達產物。
Transformation was done by electroporation . human fl extracellular domain cdna transformed to yeast host strain km71 , then his + muts phenotype transformant was screened out and cultured in flasks , and rhfl was expressed under the induction of 0 . 5 % methanol 我們提取了km71ppic9k - fl轉化菌株的基因組dna進行southern實驗，檢測目的基因的整和插入；提取總rna ，進行了northern實驗，檢測fl基因在轉化菌株中的表達。
You may choose your favorite restaurant at will to enjoy a delicious lunch or dinner ; walk along the yue man square , ngau tau kok road and mut wah street to explore the scenery of this old district ; or spend several hours to shop around happily 你可以隨意選擇一間食肆，享受一頓美味可口的午餐或晚餐，又或沿著裕民坊、牛頭角道及物華街探索這個舊區的風貌，又或在區內四處購物，輕松愉快地度過數個小時。
The area around yue man square and mut wah street has long been the heart of kwun tong since the 1950 s . it was the old market place and it remains so now , only that many more modern versatile shops have joined the scene 觀塘市中心早于50年代開始發展，當時裕民坊至物華街一帶已是觀塘居民的主要活動地點，時至今日，這里的店鋪售賣的貨品更加多元化，充分展現典型舊區的特色。
Shortly after 4 pm , a public light bus travelling along eastbound mut wah street reportedly knocked down a 73 - year - old woman when it turned left into fu yan street . the victim was trapped under the vehicle 意外發生在下午四時左右，一輛公共小巴沿物華街東行左轉入輔仁街時據報在輔仁街撞倒一名七十三歲老婦。
Two thirds of sino - foreign fund management joint ventures have yet to reach break - even , amid increasing consolidation pressure in the word ' s fastest - growing mut - ual fund market 在中國這個全球增長最快的共同基金市場上，整合壓力日漸加大，三分之二的中外合資基金管理公司尚未實現收支平衡。
Shui wo street , shui ning street , yuet wah street , tin heung street , hip wo street , mut wah street , fu yan street , yue man squane ) ngau tau kok estate ( block 10 ) 康寧道東(云漢街、嘉樂街、崇仁街、瑞和街、瑞寧街、月華街、天香街、協和街、物華街、輔仁街、裕民坊)
To find out the difference between mut + and muts recombinants we compared their expression of pokeweed antiviral protein in the same conditions 在相同的培養條件下比較了mut ~ +和mut ~ s重組菌株表達pap的異同。
But as to muts recombinants , the periods of fermentation is too long and the efficiency of using methanol is low 然而， mut ~ s菌株由于發酵周期長，甲醇率利用低，并不適用于實際的大規模生產。
The yee mut hall ( the memorial hall ) is where the memorial tablets of the deceased taoist members of sik sik yuen are placed and worshipped 意密堂安放著嗇色園已故的道教先輩的靈位；

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mut

mut n.＝ mutt.

mutability

To find out the difference between mut + and muts recombinants we compared their expression of pokeweed antiviral protein in the same conditions 在相同的培養條件下比較了mut ~ +和mut ~ s重組菌株表達pap的異同。

But as to muts recombinants , the periods of fermentation is too long and the efficiency of using methanol is low 然而， mut ~ s菌株由于發酵周期長，甲醇率利用低，并不適用于實際的大規模生產。

The yee mut hall ( the memorial hall ) is where the memorial tablets of the deceased taoist members of sik sik yuen are placed and worshipped 意密堂安放著嗇色園已故的道教先輩的靈位；