M - morula 中文 - EN166

morula n.(pl. -lae ) 【生物學】桑椹胚；桑椹體。 ...

morus
The mouse morulae were cryopreserved by vitrification in ops ( experiment 5 ) or straw ( experiment 6 ) followed by splitting . the fresh embryos undergoing the same procedure served as controls the rate of survival demi - embryos after splitting and the rate of demi - embryos developed to blastocysts in experiment 5 and 6 were similar ( p > 0 . 05 ) to those in controls , but the blastocyst rate in controls ( 77 . 38 % ) was a little higher than that in experiment 5 and 6 ( 70 . 22 % and 68 . 52 % ) 對細管法、 ops法冷凍后的胚胎進行分割，二者分割成功率、半胚培養后的囊胚發育率與新鮮對照組無顯著性差異( p 0 . 05 ) ，但鮮半胚的囊胚發育率( 77 . 38 )略高于細管法( 68 . 52 )與ops法( 70 . 22 ) 。
Somatic nuclear transfer of black bear - cat inter - species 1 ) the donor of black bear fibroblast cells , which had been treated with serum starvation for 3d , 4d , 5d , and done with the 10g / ml nocodazole for 24h , were used to recombine black bear - cat nuclear transfer embryos . after the recombined embryos were activated by 6 - dmap + ccb + ethanol , the development rates of morula were 20 . 22 % , 18 . 31 % , 17 . 65 % , 9 . 76 % , 6 . 89 % ( control ) , respectively 用乙醇6 dmap ccb 、乙醇chx ccb 、 sr ’ ” ccb等3種方法激活重組胚胎，結果表明， 3種激活方法對黑熊家貓的核移植胚胎都有激活作用，其中乙醇6 dmap ccb的激活效果最好，在tcm99成熟培養液中培養36h后，卯裂率達到66
164 microinjected embryos and 121 noninjected embryos were transferred to 14 recipient gilts . of this , 159 microinjected and noninjected embryos ( blastocyst , early blastocyst and morula ) was transferred to 6 recipient gilts , three pigs are pregnant ( one gilt delivered 8 piglets , two are skin pigment chimera ) , and pregnancy and chimera rate is 17 % ( l / 6 ) . 126 microinjected and nonmicroinjected expanded blastocysts oestrused on time , pregnancy and chimera rate is 0 ( 0 / 5 ) 其中， 159枚囊胚、早囊及桑椹胚混合胚移植到6頭受體， 3頭妊娠(其中一頭母豬產仔8頭，兩頭為皮膚色素嵌合體) ，妊娠嵌合率為17 % ( 1 / 6 ) ； 126枚擴張混合囊胚移植到5頭受體均按時返情，妊娠嵌合率為0 ( 0 / 5 ) 。
Mouse blastocysts and morulas were cultured in rat heart conditioned medium ( rh - cm ) . as far as the following indexes attaching ratio , icm growth ratio and es clone formation ratio are concerned , the choice of blastocysts is much better than morulas ( p < 0 . 05 ) , therefore mouse blastocysts were more suitable than morulas to get es cells 源于icr小鼠icm的es細胞分離克隆研究試驗以rh - cm為培養基培養處于不同發育階段的小鼠胚胎，發現由囊胚分離的es細胞傳代后es集落的出現率顯著高于桑椹胚( p 0 . 05 ) ，故囊胚更適合作為es細胞分離克隆的材料。
The demi - embryos derived from vitrified morulae in ops ( experiment 7 ) and fresh morulae ( experiment 8 ) after cultivation for 24h were transferred to day 2 . 5 to day 3 pseudo - pregnant female mice . in experiment 7 , 15 fetuses were obtained from 5 of 11 recipients which had received 191 demi - embryos 對ops法冷凍桑椹胚和新鮮桑椹胚分割后移植，將各自分割所獲得的半胚191枚和182枚，分別移植給11只和10只假妊娠2 . 5 3d的受體母鼠。
In the 2 - step ops method , embryos were pretreated with 10 % eg + 10 % dmso for 30s before exposed to edfs30 , a vitrification solution , for 25s , then immersed in liquid nitrogen . the blastocyst rate of vitrified mouse morula after equilibration in 0 . 5mol / l sucrose for 5min was 100 % Ops二步法即胚胎預處理30s ，然后移入edfs30冷凍液中平衡25s冷凍保存，解凍后在0 . 5mol l蔗糖溶液中平衡5min ，培養后囊胚發育率為( 100 ) 。
Ppar5 expression in the uterus of ovariectomized mice was significantly stimulated by estrogen or a combination of estrogen and progesterone . ppar8 mrna was detected in oocytes and preimplantation embryos at the stages of zygote , 2 - cell , 4 - cell , 8 - cell , morula and blastocyst 在小鼠卵母細胞、受精卵、 2細胞、桑椹胚和胚泡中，可檢測到ppar表達，但在4細胞和8細胞中沒有檢測到ppar基因的表達。
Experiment i study on the embryonic development by injecting different developmental stage embryos . this experiment collected 109 embryos to observe their developmental ability by es cells microinjection into 8 - 16 cell stage morula or blastocyst 本試驗先后將109枚胚胎用于此項研究，通過將小鼠es細胞注入8 - 16細胞桑椹胚和囊胚，觀察兩個不同時期注射胚胎的發育能力。
4 ) after the activated nuclear transfer embryos had been cultured in m199 ^ and czbm for 48h , the cleavage rate can reach at 16 . 41 % and 17 . 41 % , and the morula rate were 7 . 46 % , % and 6 . 74 % , respectively 08融合率：血請饑餓法和nocodazole法處理的供體細胞構建的重組胚胎，經6 dmap乙醇ccb激活，分別獲得17 33o和17 02o的卵裂率以及6
Two stage embryos were observed their effect on production of chimera . the result shows that there is a significant different ( 0 . 01 < p < 0 . 05 ) between blastocysts ( including early blastocysts and morulae ) and expanded blastocysts 結果表明，囊胚、早囊及桑椹胚同擴張囊胚兩種注射胚胎制作嵌合體所產生的結果差異顯著( 0 . 01 p 0 . 05 ) 。
The results show that the development rates of morula treated with serum starvation for 3d , 4d , 5d are higher apparently than nocodazole group and control group ( p < 0 . 05 ) 直接注射法構建的核移植重組胚胎的桑堪胚發育率（ 25 95 ）高于電融合法（ 16 07 ），統計學分析差異顯著（ p 0
The fresh mouse morulae were bisected following decompaction ( experiment 3 ) and other morulae without decompaction ( experiment 4 ) also splitted 新鮮桑椹胚經去致密化處理后分割，其分割成功率、半胚培養后的囊胚發育率都顯著高于未處理組。
6 - dmap + ccb + ethanol can effectively activate the reconstructed rat - mouse embryos . 5 ) 225 reconstructed embryos from 2 - cell to morula were transferred into 46 recipients 23 l6 dmap乙醇ccb能有效地激活種間核移植重構胚。
In this study , we found that enucleated maturation oocytes of bovine and mouse can only support nuclear transfer embryos to develop to morula 60o和8 50o的桑堪胚發育；重組胚胎在mi99和改良的czb中培養，分別獲得16 41o和17