x
uploads/luciferase.jpg

luciferase n.【生物化學】螢光(素)酶。

luciferin

To test the p7 promoter activity , a series of constructs were obtained by cloning the different dna fragments into the luciferase gene reporter vector pgl3 - b . when the constructs were transiently transfected into thp - 1 cells , luciferase activity assay showed that the core region of p7 promoter located at - - 165 bp 第一部分的工作首先通過對人acat - 1基因p7啟動子序列進行5 '和3 ' -端的缺失的詳細分析,結果顯示最大報告基因轉錄表達活性位于- 612 - 165bp區段。

Tumor necrosis factor ( tnf ) , a cytkine that exerts many pro - atherosclerotic effects in vivo , causes up - regulation of acat - 1 gene expression in human blood monocytes . by luciferase activity assay , tnf - a enhanced acat - 1 p7 promoter activity in thp - 1 monocytic cell line 細胞因子tnf -即腫瘤壞死因子大量存在于人動脈粥樣硬化斑塊中,本實驗通過熒光素酶活性和rt - pcr測定結果表明, tnf -處理人血單核細胞和單核細胞株thp - 1均增強acat - 1基因p7啟動子活性。

Further , their luciferase activities were determined in ty medium , and showed that tn5 - 1063 was inserted directly into loci downstream of promoters in 042bm genome 它們都表現發光酶活性,表明轉座子正向插入到基因組中的某個啟動子下游。

The inhibition of prl on luciferase gene expression in the group of transfected cho cells was observed as pgl3 - l . 6 > pgl3 - 2 . 5 > pgl3 - 338 證明叩l和hcg的調控元件主要位于20ahsd基因的15s7 286之間。

Construction of adenoviral vector for luciferase driven by htert core promoter modified with myc - responsive elements 核心啟動子引導熒光素酶表達的腺病毒載體

Influence of 3 ' untranslated region single nucleotide polymorphism of tlr4 gene on luciferase gene expression 位點突變對熒光素酶表達的影響