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ligase

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ligase n.【生物學】聯結酶。

n. 【生物學】聯結酶。 “amido ligase“ 中文翻譯：   酰胺邊接酶; 酰胺連接酶“biotin- ligase“ 中文翻譯：   生物素-連接酶“coa ligase“ 中文翻譯：   輔酶a生成酶“dna ligase“ 中文翻譯：   dna連接酶,多脫氧核糖核苷酸合酶; dna黏合酶; 脫氧核糖核酸連接酶“glutamate ligase“ 中文翻譯：   谷氨酸連接酶“phosphoribitol ligase“ 中文翻譯：   d-丙氨酸聚連接“polynucleotide ligase“ 中文翻譯：   多核苷酸連接酶“rna ligase“ 中文翻譯：   rna連接酶,核糖核酸連接酶; 核糖核酸連接酶“splicing ligase“ 中文翻譯：   剪接連接酶,拼接連接酶“3ropionate-coa ligase“ 中文翻譯：   丙酸-coa連接酶“4-coumarate-coa ligase“ 中文翻譯：   4-香豆酸-coa連接酶“5-formyltetrahydrofolate cyclo-ligase“ 中文翻譯：   5-甲酰四氫葉酸環連接酶“6-carboxyhexanoate-coa ligase“ 中文翻譯：   6-羥乙酸-coa連接酶“acetate coa ligase“ 中文翻譯：   醋酸鹽輔酶a配位酶“acetate-coa ligase“ 中文翻譯：   乙酸-coa連接酶“acetoacetate-coa ligase“ 中文翻譯：   乙酰乙酸-coa連接酶“acid-coa ligase“ 中文翻譯：   酸-coa連接酶“alanine-trna ligase“ 中文翻譯：   丙氨酸-trna連接酶“amine acid-rna ligase“ 中文翻譯：   氨基酸rna連接酶“amino acid ren ligase“ 中文翻譯：   氨基酸rna連接酶“amino acid rna ligase“ 中文翻譯：   氨基酸核糖核酸連接酶; 氨基酸連接酶“amino acid-rna ligase“ 中文翻譯：   氨基酸rna連接酶“amino-acid-rna ligase“ 中文翻譯：   氨基酸核糖核酸連接酶“aminoacyl trna ligase“ 中文翻譯：   氨酰trna連接酶“ligase chain reaction“ 中文翻譯：   連接酶鏈反應; 連接酶鏈式反應“ligasacchi“ 中文翻譯：   利加薩基

ligate

First , the purified pezzis and pcr product of angiostatin are digested by ecor . i and xba i . after purifying the digested products respectively , we ligate these two kinds of dna by t4 dna ligase and construct the recombinant plasmid pezz18 - as . then transform it to the competent e . coli dh5a 用限制性內切酶ecori與xbai對目的基因as 、表達載體pezz18行雙酶切，酶切產物純化后利用大腸桿菌t _ 4dna連接酶連接構成重組子pezz18 - as ，并轉化e . colidh5 ，經氨芐青霉素lb平板初篩后，以菌液pcr和重組子的單、雙酶切行進一步鑒定。

The scfco , “ ubiquitin - ligase complexes are required for jasmonate response in arabidopsis ( l ) arabidopsis coi is required for all the jasmonate - regulated response . it encodes a protein containing leucine - rich repeats and a degenerate f - box motif these structural features are characteristic of f - box proteins that function in ubiquitin ligase complexes for the ubiquitylation of substrate - proteins targeted for degradat1on 以表達融合蛋白flag - coi1的擬南芥為材料，用- flag抗體進行免疫共沉淀分析發現， coi1蛋白可以與ask1 、 ask2 、 atrbx1和atcul1蛋白結合形成scf ~ ( coi1 )復合體，并發現其中的atcul1蛋白包括未修飾和修飾的兩種形式。

A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a , yielding a 1 . 7kb band . the segment was linked to puc19 plasma dna by means of t4 dna ligase , transformed into e . coli jm109 permissive cells , and incubated on lb fray containg amp , x - gal and iptg . small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr , resulting in recombinant plasma puge dna containing prv ge 用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落于選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，并進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，并與其它prv分離株進行ge基因序列同源性分析。

This time , using cdna of zmcdc5 as template , we amplify a sequence by means of pcr technology . and then , using restrict endoenzyme and ligase , we conjunct the 0 . 8kb length dna sequence in a expression vector , pet - 30a . after induction , expression and purification , we obtained a 35 . 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ) . with the purified protein , we got its antibody and testified the antibody by means of western blotting and dot blotting 本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建于pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基

After digested with ecori and noti , the obtained dna fragment was linked with ppic9k by t4dna ligase and then the recombinant plasmid was transformed into competent dhscc . after positive transformants were sieved out by pcr , digesiting analysis and sequencing were also used to confirm the positive result more 所得的dna片段經ecor和not雙酶切后用t _ 4dna連接酶與ppic9k載體進行連接，然后導入大腸桿菌dh5 ，用pcr法篩選陽性轉化子，并用雙酶切和序列測定方法鑒定重組質粒。

This study demonstrated that the arabidopsis f - box protein coil associated with atcul1 , atrbxl and skpl - like proteins askl and ask2 to assemble scfcoil ubiquitin ligase complexes . also , we found that the atcull component of scfcoil complexes contained two species including atcull and modified atcull . ( 2 ) we found that coil assembled to two separate scfcoil complexes with either askl or ask2 through immunoprecipitation analysis with plant expressing myc - tagged version of ask2 用表達融合蛋白myc - ask2的擬南芥為材料，以- myc抗體進行免疫共沉淀分析發現， myc - ask2蛋白可以與coi1蛋白一起免疫共沉淀，但是不能與ask1蛋白免疫共沉淀，表明coi1蛋白與ask2蛋白，但是不能同時與ask1結合形成scf ~ ( coi1 )復合體。

The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme , were linked by means of t4 dna ligase and transformed into e . coli jm109 permissive cells , yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid 將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理， t _ 4dna連接酶連接，用連接產物轉化大腸桿菌jm109感受態細胞，得到重組轉移載體質粒pfastbac - gedna 。

At first . then eight a - amylase gene fragments were cloned with the genomic dnas as templates by routine pcr . following that , these gene fragments and plasmid vectors , pbluescript ii ks + and puc18 , were cut by bamh i and kpn i . the prepared insert dna and vector dna were linked by t4 dna ligase 利用vectornti6 . 0軟件，對所克隆的序列用相鄰接點法( neighborjoining州j ） method ）進行多序列比對，分析其同源性，并構建基因進化樹。

Cut off beta fragment from plasmid prok . ii with hindlll and ecor i as insert , and cut pa into linear plasmid as vector fragment . link the insert and vector fragment together with t4 ligase , and the new vector with gene beta and gus was constructed 用hind和ecor雙酶切prok質粒，獲得beta基因片段作為插入片段，用hind和ecor雙酶切a質粒作為載體片段，將插入片段與載體片段相連，即構建成含有beta和gus的雙基因載體。

Sub - - clone of s , . / hbsag fusion gene : pbuescripts , . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme , and were linked under t4 dna ligase , ppiczaa s , , / hbsag was constructed and transformed to e . coli Hbsag質粒與ppiczaa載體分別經xhol和xbaln切，再在t4dna連接酶作用下進行連接，獲得工程菌表達型ppiczaas ； hbsag質粒，轉化大腸桿菌t0p10細胞，經xhol和xbal與sacll和xbal酶切電泳，證實s ； 。

4 . the construction of middle - clone vector and expression vector the puc - cp and pgem - 7z plasmid were digested by kpnl and bamhi , and collected the digested cpti fragment and the pgem - 7z , then ligated by t4 dna ligase and formed the pgem - cp 中間載體及表達載體的構建將puc - cp質粒和pgem ? 7z質粒，用kpni和bamhi酶切，分別回收cpti片斷和酶切后的載體片段，用t _ 4連接酶連接構建成中間載體pgem - cp 。

As bases are added by polymerase to the starting point of a new complementary strand , known as a primer , or recognized by ligase as a match , the template ' s sequence is revealed 當聚合酶將一個核苷酸加在新互補鏈的起始引子之后，或接合酶認定某段核苷酸鏈與原始模版配對，就可利用這些反應來得出原始模版的序列。

Then cdnas and puc18 vectors were linked by t4 dna ligase and transformed into e . coli strain dh5 - alpha to generate cdna library that size is 4 . 9 l06 recombinants 將cdna與載體連接，并導入dh5感受態細胞中，構建成cdna文庫。

Ligase an enzyme that catalyzes the bond formation between two substrates at the expense of the breakdown of atp or some other nucleotide triphosphate 連接酶：可將兩個底物結合在一起的酶，此過程需要atp或其他核苷三磷酸供能。

We have identified in s . cerevisiae distinct ubiquitin - ligase complexes that define different erad pathways 我們在酵母體內發現了一種參與不同erad途徑的特定的泛素連接酶復合體。