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lactase n.【生物化學】乳糖酶。

lactate

Klac gene was ligated to the pet - 30a ( + ) vector for expression . then the recombinant plasmid petlac4 was transformed into e . coli bl21 . the positive transformants were induced at different temperature for three hours by iptg with the final concentration of lmm . sds - page analysis and lactase activity assay showed that klac gene was expressed in e . coli , and that the expression level at 28 was much higher than that at 37 表達產物的sds - page分析和酶活性測定表明, klac基因在大腸桿菌中獲得活性表達,其中低溫條件28下的表達水平明顯高于37 , 28誘導的細胞經超聲波破碎所測酶活力為0 . 475u / ml , 37僅為0 . 134u / ml 。

So the lactase was suitable to whey at ph7 . 0 , but could n ' t react at high tempreture . the metals , fe2 + , mn2 * , ca2 + , mg2 + and zn2 + , can prominently improve lactase activity , but cu2 + would strongly inhibit the activity . according to the effect of edta , we concluded that the lactase depends on metal ions in a certain extent 乳糖酶基因klac在巴斯德畢赤酵母中表達將klac基因插入到載體ppic9上構建重組表達質粒pic9154983 ,電擊轉化巴斯德畢赤酵母受體菌gs115 ,經篩選和pcr鑒定, 1 # 、 8 #和24 #為重組菌株, klac基因整合到gs115基因組中。

Klac gene was inserted into the vector ppic9 to construct recombinant plasmid p1c9154983 , and then pic9154983 was transformed by electricity pulse into gs115 , pichia pastoris acceptor . after screening , we obtained recombinant yeast 1 # , 8 # and 24 # , that is , klac gene was integrated into the genome dna of gs115 . by detecting the lactase activity , we knew that the klac gene was expressed in vivo of pichia pastoris , but the lactase was not secreted 三個重組菌株經甲醇誘導培養未檢測到分泌的乳糖酶,但胞內均檢測到有活性的乳糖酶,分別為0 . 1392u / ml 、 0 . 3702u / ml和0 . 2214u / ml ,說明乳糖酶基因klac在巴斯德畢赤酵母中獲得表達。

The lactase gene from kluyveromyces lactis was researched in the thesis . the cloned gene was expressed in e . coli and the properties of lactase was determinated . in addition , we studied the expression of lactase gene in the methylotrophic yeast pichia pastoris 本論文從一株乳酸克魯維斯酵母菌中克隆獲得乳糖酶基因,在大腸桿菌中進行表達并測定其酶學性質,同時也對利用巴斯德畢赤酵母( pichiapastoris )系統表達該基因進行了探索。

Preparing the lactase solution expressed in e . coli , we determinated the optimum ph , ph stability , optimum reaction tempreture , thermal stability , effect of metal ions and chemical reagent , km and vmax its optimum ph was 5 . 8 and 6 . 4 to 7 . 5 , and the optimum tempreture was 45 to 52 以onpg為底物,分別測定了其最適ph值及ph穩定性、最適溫度及熱穩定性、金屬離子和化學試劑的影響以及k _ m值和v _ ( max )值。

Cloning and expression in e . coli of lactase . specific primers were designed according to the sequence of the beta - galactosidase gene from kluyveromyces lactis . klac gene was amplified by pcr and subsequently cloned 乳糖酶基因的克隆及原核表達以乳酸克魯維斯酵母( kluyveromyceslactis )菌株k538的基因組dna為模板,設計引物,利用pcr獲得乳糖酶基因klac ,經dna測序驗證,得到克隆t1549 。

This disorder , apparent within 10 days of birth , occurs when brush - border lactase activity ( required for the digestion of lactose ) in the small intestine is low or absent at birth and is characterized by diarrhea and malabsorption 此病是由于寶寶出生時小腸內的乳糖分解酶素活性很低或沒有活性,病癥通常在寶寶出生10天內顯現:拉肚子以及吸收不良。

At present , most of lactases used in industry production come from kluyveromyces , aspergillus niger and aspergillus oryzae 目前,工業生產中使用的乳糖酶主要來源于乳酸克魯維斯酵母菌、黑曲霉和米曲霉。

Some premature babies have a temporary form of lactose intolerance because their bodies are not yet producing lactase 一些早產的寶寶會暫時的有乳糖不耐受癥狀是因為他們體內還未開始分泌乳糖分解酶。

The former was 0 . 475u / ml , and the later was 0 . 134u / ml . 2 . determination of the properties of lactase expressed in e . coli 原核表達乳糖酶的酶學性質測定制備原核表達乳糖酶的原酶液。

Many asian adults are lactose intolerant , as a result of a lack of the digestive enzyme lactase in the gut 許多亞洲成年人有乳糖不耐受癥,因為他們消化道中缺乏消化乳糖的乳糖酶。

A similar , less severe , metabolic disorder is congenital lactase deficiency 類似的,病癥略輕為一點的代謝紊亂疾病是乳糖分解酶素缺乏癥。

The influence of ca2 fe2 mg2 zn2 na and k on lactase activity in sucking piglets 對哺乳仔豬乳糖酶活力的影響

Study of the relationship between urticaria diarrhea and lactase 藥物性腹瀉病與乳糖酶的關系研究

Study of isolation and purification of lactase from aspergillus niger d 226乳糖酶分離純化研究