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kanamycin

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kanamycin n.【藥學】卡那霉素。

n. 【藥學】卡那霉素。 “kanamycin b“ 中文翻譯：   氨基去氧卡那霉素; 卡那霉素b; 卡乃多霉素“kanamycin base“ 中文翻譯：   卡那霉素堿“kanamycin c“ 中文翻譯：   卡那霉素c“kanamycin kinase“ 中文翻譯：   卡那霉素激酶“kanamycin monosulfate“ 中文翻譯：   單硫酸卡那霉素; 硫酸卡那霉素“kanamycin sulfate“ 中文翻譯：   硫酸卡那霉素“kanamycin sulphate“ 中文翻譯：   硫酸卡那徽素; 硫酸卡那霉素“kanamycin-vancomycin“ 中文翻譯：   卡那霉素-萬古霉素“carbamoyl-kanamycin b“ 中文翻譯：   酰氨卡那霉素 b“kanamycin acid sulphate“ 中文翻譯：   重硫酸卡那霉素“kanamycin acide sulphate“ 中文翻譯：   硫酸卡那霉素“kanamycin b sulfate“ 中文翻譯：   硫酸卡那霉素b“kanamycin eye drops“ 中文翻譯：   卡那霉素滴眼劑“kanamycin mono sulphate“ 中文翻譯：   單硫酸卡那霉素“kanamycin sulfate for injection“ 中文翻譯：   注射用硫酸卡那霉素“kanamycin sulfate injection“ 中文翻譯：   硫酸卡那霉素注射液“kanamycin sulphate for injection“ 中文翻譯：   注射用硫酸卡那霉素“kanamycin-vancomycin blood agar“ 中文翻譯：   卡那霉素萬古霉素血瓊脂,kvba培養基“laked kanamycin vancomycin“ 中文翻譯：   使血球溶解的卡那霉素,萬古霉素“neomycin-kanamycin phosphotransferase“ 中文翻譯：   新霉素-卡那霉素磷酸轉移酶“kanamycin-vancomycin laked blood agar“ 中文翻譯：   卡那霉素萬古霉素血球溶解血瓊脂,kvlb瓊脂“kanamyci“ 中文翻譯：   卡那霉素“kanamura“ 中文翻譯：   金村“kanamugire“ 中文翻譯：   卡納穆吉雷“kanamu“ 中文翻譯：   含藝“kanampilly“ 中文翻譯：   卡南皮利

kanarese

The chimeric gene was introduced into arabidopsis through the method of vacuum infiltration which is widely used in arobidopsis tansformation . transgenic plants were screened by means of kanamycin resistance and further identified by genomic pcr and western blotting , through the phenotype observation of transgenic plants , the speculation that apoplast calmodulin may play a certain role in regulating the development and growth of plants was made 最后，融合基因被插入二元載體plbj21 ，通過電擊法轉入農桿菌gv3101 ，真空滲入法轉入了擬南芥，轉基因擬南芥通過kan抗性篩選而得到，并進一步進行了基因組pcr鑒定和western雜交的鑒定。

Our experiment indicates : ( 1 ) the optimal concentration of kanamycin for screening torenia fournier regenerated buds was 400 mg / l . the ideal transformation was obtained in the following conditions : the leaf discs were dipped in agrobacterium suspension that od600 was 0 . 1 for 10 ~ 20 min ; subsequently cocultivated on the ms solid coculture medium containing 20umol / l acetosyringon for 7 to 8 d at 23 , and the induction ratio of regenerated buds was 27 . 2 % 研究結果表明： ( 1 )篩選藍豬耳轉化芽的最適卡那霉素濃度為400mg l 。 od _ ( 600 )為0 . 1的菌液濃度菌液浸染葉盤10 20min ，固體共培養基(含20 mol l乙酰丁香酮)上23共培養7 8d可獲得理想的轉化效率，轉化芽誘導率為27 . 2 ； 16h光照， 8h黑暗是較理想的共培養光周期；莖段是較好的轉化受體。

The results indicated that all isolates exhibited a susceptibility to amikacin and ceftriaxon , and 67 isolates showed a greater or lesser degree resistance to streptomycin , kanamycin , gentamicin , ampicillin , tetracycline , chloramphenicol , florfenical , cefotaxime , cephalothin and ceftiofur , to which 22 isolates exhibited a susceptibility . some isolates showed resistance to multiple antibiotics and displayed a highest level resistance to streptomycin with a frequency of 43 . 8 % , followed by tetracycline with a frequency of 30 . 3 % 結果表明，所有分離菌株均對阿米卡星和頭孢曲松敏感； 67株對鏈霉素、卡那霉素、慶大霉素、氨芐西林、四環素、氯霉素、氟苯尼考、頭孢噻肟、頭孢噻吩、頭孢噻呋表現出不同程度的耐藥性，其中對鏈霉素的耐藥率最高，為43 . 8 ，其次為四環素( 30 . 3 ) ，其余22株為敏感菌株。

The resulting plasmid , named prok - sod2 , was mobilized to agrobacterium tumefaciens strain gv3101 used for plant transformation . the yeast sod2 gene was introduced into arabidopsis thaliana ( ecotype landsberg erecta ) by agrobaterium tumefaciens - mediated transformation with floral - dipping method under the control of camv 35s promoter . transformants were selected for their ability to grow on medium containing kanamycin ( 30mg / l ) , several homozygous lines that were all tolerant to kanamycin were selected and used for further molecular and physiological determination 本實驗將sod2基因構建到植物表達載體prok中，導入農桿菌后，進行植物遺傳轉化，實現其在擬南芥中過量表達，在含30mg l的卡那霉素的培養基上篩選獲得純合轉基因株系，自交一代獲得足夠的純和轉基因種子后，對其進行了分子生物學的驗證及生理指標的檢驗。

Establishment of carrot genetic transformation an efficient transformation protocol based on agrobacterium tumefacien lba4404 was created in the study . good results were gained when use fresh hypocotyls as infectious explants , co - cultivate in medium supplemented with low concentration of acetosyringone ( 25 m ) , screen in medium with loomg / l kanamycin . it is time saving when anti - culli are fisrt screened in low concentration of antibiotics then transfered to high concentration ones , and remove antibiotics when regenerate 建立了高效的遺傳轉化體系以pbi121和ptok233為轉化質粒，在農桿菌lba4404介導下，對胡蘿卜遺傳轉化體系進行系統研究，首次為該胡蘿卜品種建立一套高效的遺傳轉化體系，結果為:最適轉化受體為新鮮下胚軸及經預培養的下胚軸;共培養時，低濃度的乙酞丁香酮( 25pm )對轉化具有促進作用;適宜的卡那霉素篩選濃度是loom歲l 。

The chloroplast shsp gene was screened from the cdna library of tomato flower by pcr strategy and confirmed by sequencing . but difference was found at 3 bases of the sequence from the reported in genbank . then , an integrated vector prok ii of the chloroplast shsp gene and nptii gene ( a kanamycin resistant gene ) with camv35s promoter was constructed and introduced into tomato mediated by agrobacterium tumefaciens lba4404 . transgenic tomato were screened by their ability of growing on media containing kanamycin 本實驗采用pcr方法從番茄花cdna文庫中克隆到葉綠體shsp基因，經測序證實與genbank中已發表的序列在編碼區相差2個堿基，其中一個堿基導致1個氨基酸的改變。將葉綠體shsp基因定向克隆于帶有組成性表達啟動子camv35s的植物表達載體prok中，凍融法轉化農桿菌lba4404 ，利用葉圓盤法對番茄進行ti質粒介導的遺傳轉化。

Transformed shoots were selected on solidified medium containing kanamycin . ten kanamycin resistant transformants were obtained by direct or induction calla . these transformants were checked by pcr , pcr - southern blot and southern blot , confirmed that two positive transformants were integrated into the genome of boechmeria nivea l guad 對所得到的抗性轉化株進行了pcr 、 pcr - southernblot 、 southernblot檢測，其中2株呈陽性，證明vp4基因已整合到苧麻基因組dna中。

Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ) . nineteen individual kanamycin resistant plants were obtained . t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l ) 將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中，導入根瘤農桿菌gv3101后，由花浸泡法進行擬南芥遺傳轉化，轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮霉素（ 25mg ）的ms培養基上篩選，獲得t ；代轉基因植株。

6 . transformation system of mustard a serials of kanamycin concentration was added to optimum medium to test the explants resistance capacity of two kinds of mustard . the transformation procedures described were derived from numerous regeneration and trasformation designed to test factors that might affect shoot regeneration , which including length of co - cultivation . those producing the best result parameters were described as below : after the mustard explants were precultured on regeneration medium for 2 days . they were inoculated with agrobacterium for 20 minutes . inoculated explants were co - cultivated for 4 days and in shadow at first 2 days . then transferred to the same medium plus 30 mg / l kanamycin and 500mg / l garb . all of them were transferred to fresh medium every 2 weeks . the kan - resistant plants were regenerated 芥菜外植體高頻遺傳轉化體系的建立在最適培養基上試驗了兩類芥菜的三種外植體對卡那霉素的敏感性、預培養天數、浸菌時間等因素的影響，建立了芥菜高頻轉基因再生體系：取生長4天的芥菜子葉、下胚軸和25天的葉片在分化培養基上( ms + ba3 . 0mg / l + naa0 . 1mg / l )預培養2 - 3天后，投入農桿菌菌液中浸染20分鐘，在分化培養基上暗培養2天，正常條件下培養2天后，轉入抗性培養基（ ms ba3

3 . potato stems and agrobacterium fumefaciens containing recombinant vector were co - cultured at 28cfor 48 hours and transplanted onto callus - inducing medium at 24c for 7 days . and then , the explants were transplanted onto differentiation medium and cultured at 24c for 21 days . resistant buds rooted and grew into plants in medium with kanamycin for 20 days , and 83 plants were obtained 3將含外源基因的根癌農桿菌與馬鈴薯莖段共培養后在愈傷誘導培養基上培養7天，轉接到分化培養基上分化出抗性芽，抗性芽在生根培養基上生根長成完整植株，共獲83株。

One to offspring ( t1 ) was selected for further genetic analysis from two types of self - pollinated transgenic plants and screened for the presence of the recombinant gene by the pcr respectively . the result suggested that the resistance to kanamycin can be a stable heredity by genetic analysis , separation ratio of t1 was 3 : 1 and t1 kanamycin - resistant tomato plants can carry vp7 gene 此外，從兩種轉基因植株中分別選取一自交株進行t _ 1代遺傳分析，其kan抗性基因分離比皆為3 : 1 ， t _ 1代抗性番茄植株的pcr結果也呈陽性。

The regeneration system of soybean cytoledon node and agrobacteriunr mediated transformation method is the first selection at present . in the second part of this experiment , the expression vector prok2 containing npt ii and ssnhx1 ( na + / h + antiporter ) gene from suaeda salsa was introduced into soybean cytoledon nodes by gene transformation mediated by agrobacterium tumefaciens , and kanamycin resistant transgenic p lants were obtained by screening in selective condition 本實驗第二部分通過農桿菌介導法將含npt -和鹽地堿蓬na ~ + h ~ +反向轉運蛋白基因( ssnhx1 )的表達載體prok2導入大豆子葉節中，經過含km的篩選培養基連續篩選，獲得了ssnhx1轉基因植株，篩選劑卡那霉素的適宜濃度是50mg . l ~ ( - 1 ) 。

The growing graphs of e . coli bl21 containing pbv221 and pbv221svhb according to the od600 values showed that the e . coli vgb + grew faster 4 the highly - efficient bivalent vectors which are named pgbif4abcvhb and pgbi4asvhbbt respectively , havfs been . su ? essful ] y constructed . the tobacco leave discs were transformed with agrobacterium tumefaciens carrying the plant expression vectors pgbif4abcvhb and pgbi4asvhbbt . 38 and 36 transgenic tobacco plants have been obtained respectively after kanamycin screening , pcr detection , southern blot analysiss showed that the foreign genes have been inserted into the tocacco plant genome . the expression of 16kd vhb protein in transgenic plants was proved by western blot 4構建了雙價基因高效植物表達載體pgbif4abcvhb和pgbi4asvhbbt 。通過農桿菌介導法，將這兩個雙價基因高效表達載體導入煙草，分別獲得了38 、 36株陽性轉基因植株。經pcr 、 southernblot分子生物學方法分析，證明vgbm ， bt基因、 bt - cpti基因已整合到煙草基因組中， westernblot證明vgbm成功地表達出16kd的vhb蛋白。

Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and loomg / l kanamycin and incubated at 25 , 16 / 8h light / dark cycle . small leaves of adventitious shoots differentiated from explants were cut and dipped into gus staining solution . positive shoots , gus tinted , were induced to root 分化出的不定芽切下半張葉片進行gus染色，呈陽性的植株從外植體上切下，在生根培養基（ ms 0刀sing lnaa 50mg幾kan ）中誘導根的形成。

Promoter - probe vector psupv4 was used to clone promoters of pseudomonas pseudoalcaligenes in escherichia coli . nine kanamycin resistant recombinants were obtained and designated as ppal - ppa9 . the ppa7 , which has the highest kanamycin resistance , was chose for further characterization 利用啟動子探針型載體psupv4直接在大腸桿菌中克隆類產堿假單胞菌( pseudomonaspseudoalcaligenes )基因啟動子片段，獲得具有強啟動子的重組子ppa7 。

The high performance two - gene expression vector pc3c ! c2 was transformed to nicotiana tobacum honghuadajinyuan plants mediated by agrobacterium tumefaciens eh a105 according to the leaf disc procedure . transformed shoots were selected on solidified medium containing l00mg / l kanamycin 將含有phac1和phac2雙基因的植物高效表達載體pc3c1c2 ，用凍融法，構建了pc3c1c2根癌土壤桿菌( agrobacteriumtumefacienseha105 )的工程菌。

At the same time , phacl gene was modified by pcr . three plant expression vectors containing kanamycin photransferase gene : pc3cl ( containing phacl ) , pc3cl ( containing phac2 ) , pc3c ! c2 ( containing phacl and phact ) were constructed 同時利用套疊pcr技術對phac1基因進行了改造，經過基因拼接構建了帶有卡那霉素抗性基因的植物表達載體pc3c1 (嵌合phac1 ) 、 pc3c2 (嵌合phac2 )和pc3c1c2 (嵌合phac1和phac2雙基因) 。

The seedlings were then growing on the medium for 20 days . the primary results show that the tolerable concentration of kanamycin to the tested seedlings is up to 15ug / ml and the suggested concentration for selection of resistant plant is 25ug / ml 為篩選卡那霉素抗性的轉基因植株，還進行了甘藍型油菜卡那霉素敏感性實驗。將正常甘藍型油菜種子表面消毒后，接種于不同濃度卡那霉素ms瓊脂培養基上，光照培養、篩選。

The co - cultivation had been carried out for 2 ~ 3 days . after that , the transformants were obtained by transferring explants to selection medium containing 75mg / l kanamycin and rooting medium contain - - ing 75mg / l , 100mg / l kanamycin . and its rooted frequency was 82 . 5 % 為75mg幾、 100mg l的培養基上進行生根篩選，生根率為82 5 。轉入外源基因的轉化體將會在這一系列篩選過程中發芽、生根，獲得轉基因植株。