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isoelectric adj.【物理學】等電位的,零電位差的。 isoelec...

isoelectronic

Firstly , the eo genes of csfv - jl and csfv - ln9912 strains were amplified by rt - pcr and nested pcr and then sequenced after cloned into t easy vector . comparing the eo sequences with other strains eo by biosoftwares , dnastar5 . 0 and bioedit , the phylogenetic analyse revealed that all of the strains we have sequenced could be divided into groupl and groupii . two amino acid streches of 15 csfv strains eo , which form the rnase active site , and histidine residues essential for rnase catalysis in both ones were highly conserved . the eo protein propertys of antigen epitope , hydrophobicity , isoelectric point were also predicted by bioinformatic method 首先,采用rt - pcr和nest - pcr技術擴增了csfv - jl株和csfv - ln9912株eo基因,插入teasy載體后測序,應用dnastar5 . 0和bioedit軟件將其與本實驗室已測其它毒株和genebank中登錄的csfv代表毒株eo基因序列進行比較,繪制了遺傳進化樹并預測了eo蛋白的抗原表位、疏水性、等電點等特性。

Abstract : three theory problems about technology of leather making are discussed . the first is the difference between surface charge and isoelectric point ( pi ) . the second is about the origin of the calculation formula of basicity in making cr3 + liquor . the third is the swelling mechanism of a skin or hide in basic medium 文摘:本文討論了皮革制造中的三個理論問題,一是表面電荷與電點( pi )之間差別,二是三價鉻鞣液配制中公式的簡單來源,三是生皮在堿液中膨脹的機理。

The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd , 120 kd and 91 kd , respectively . we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5 . 5 and 5 . 7 . immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph , which as vn was deposited in the ovary , while not in the male Page電泳結果表明:麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白,分子量分別為181kd和136kd ; sds - page電泳分析:存在3條雌特異性帶,其分子量為123kd 、 120kd和91kd ,由此,可推定卵黃原蛋白( vitellogenin , vg )和卵黃磷蛋白( vitellin , vn )由2個蛋白組成,其中分子量較大的蛋白由2個亞基組成;雙向電泳結果顯示,在120kd附近有兩個特異性點,其等電點為5 . 5和5 . 7 ;雙擴散表明,麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉淀反應,而與雄蜂血淋巴無免疫反應,說明了vg與vn具有免疫同源性,是雌特異性蛋白,且由脂肪體合成。

Fourthly , according to the activity , collect , dialyse , freeze , dry respectively the sod protein through deae - sepharose column chromatog - raphy ; process the sod protein through sephacryl s - 200 column chromatography with the preceding method . at last , process the pure sod protein with same functional enzyme electrophoresis and active dye , isoelectric focusing electrophoresis and sds - page 將粗酶液過deae -瓊脂糖柱層析,得三個活力峰,分別收集、透析、干燥濃縮后;再上sephacryls - 200凝膠柱層析,按與deae -瓊脂糖柱層析后同樣的方法收集處理。

Methods : in the present experiment , acid dehydroxy amino acid analysis and isoelectric focusing in polyacylamide gels were used and the amino acid composition and isoelectric point of porcine platelet - derived growth factor were studied 方法:用酸水解及聚丙烯酰胺凝膠等電聚焦的方法測定豬血小板衍生生長因子的氨基酸含量、組成成分和等電點。

The water flux decreases with increasing ph and increases with 1 . the bsa rejection is highest at the isoelectric point ( iep ) ( 4 . 8 ) , while decreases on both sides of iep . the bsa rejection increases as i decreases 在低離子強度(高ph值)溶液中,分子鏈伸展,膜通量較小;而在高離子強度(低ph值)溶液中,分子鏈收縮,膜通量較大。

At last , we got the pure protein with the determination of electrophoresis . the molecular weight of the enzyme is estimated at 30903 through gel filtration and sds - page electrophoresis . the isoelectric point is 8 . 6 ? . 3 經sds凝膠電泳和凝膠過濾層析兩種方法測定該酶的分子量分別為33113dalton和28840dalton ;其等電點pi = 8 . 6 0 . 3 。

It also had common features of plant nsltps ( non - specific lipid transfer proteins ) such as eight conserved cysteine positions , high isoelectric points ( 8 . 9 ) and lack of tryptophans 其推測的氨基酸序列與genbank中注冊的ltp蛋白前體的同源性達63 ,且具有植物lh蛋白共同特征,如8個保守的半肌氨酸位點,較高的等電點oi 8

After being concentrated and desalted , the samples were separated by isoelectric focusing on first dimension and sds electrophoresis on second dimension 樣品濃縮除鹽后,進行一維等電聚焦分離和二維sds電泳分離提取人腎組織磷酸化蛋白。

Isoelectric focusing a technique used in electrophoresis to separate amphoteric molecules ( able to combine with either acids or bases ) 等電點聚焦:是一種利用電泳技術分離兩性分子(能與酸或堿結合)的技術。

After quantification , the protein samples were separated by isoelectric focusing electrophoresis and then by sds - page 定量后,分別進行一維等電聚焦分離和二維聚丙烯酰胺凝膠電泳分離。

After isoelectric focusing , it gave a band at ph 8 . 25 . agglutination test suggested that the protein was a lectin 由于殼聚糖結構的特殊性,因此,它可被用來作為親和層析的載體

Objective : to determine the amino acid composition and isoelectric point of porcine platelet - derived growth factor 摘要目的:測定豬血小板衍生生長因子的氨基酸組成和等電點。

Differences of seed protein from rice oryza spp . varieties identified by ultrathin layer isoelectric focusing 法對番茄雜交種子純度和真實性鑒定的研究

Varietal identification of oat avena spp . by ultrathin - layer isoelectric focusing of seed protein 超薄層等電聚焦電泳技術檢驗燕麥種子的真實性

This ph is termed isoelectric point , at which proteins show their minimum solubility 這個ph稱為等電點,蛋白質在這一點上溶解度最小。

Capillary isoelectric focusing cief 毛細管等電聚焦

This ph is termed isoelectric point, at which proteins show their minimum solubility . 這個PH稱為等電點,蛋白質在這一點上溶解度最小。

A virus is negatively charged above its isoelectric point . 病毒在它的等電離點以上時帶負電荷。