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inoculum n.(pl. -ula) 接種體,接種物,種菌 (=in...

inodorous

A . niger m - l which was screened in our laboratory produced a strongly a - transglucosidase . in this paper , studies on the fermentation conditions , purification and characterization of a - transglucosidase and its necessary groups was carried out in this dissertation . the main reports were as following : the fermentation conditions in shaking flasks were investigated by the method of single - factor analysis , the suitable main medium was achieved : which contained 4 % a , 2 % b and 1 % g ; the a . niger m - l was inoculated into 100ml medium in flask , shaking in 33 c at 140r / min for four days , with initial ph6 . 5 and 6 % inocula volume ; adding 0 . 1 mmol / l methyl a - d - glucopyranoside had inductive effect on enzyme formation , the a - transglucosidase activity amounted to 296 . 05u / ml 本研究以黑曲霉m - 1為出發菌株,對其-葡萄糖轉苷酶的產酶影響因素、純化、酶學性質以及必需基團進行系統的研究,結果如下:通過對影響黑曲霉m - 1產-葡萄糖轉苷酶的單因素分析,得液態發酵生產-葡萄糖轉苷酶的最適產酶條件為: 4 a , 2 b和1 g ;在33 ,起始ph值為6 . 5 ,轉速為140r min ,接種量為6 ,裝液量100ml條件下,發酵4 . 0d ,酶活力達296 . 05u ml ,添加0 . 1mmol l的酶作用底物甲基- - d -葡萄糖苷對產酶的誘導作用最大。

The optimized result of cell immobilization cultures was acquired , including the support matrices pretreatment , the class and size of support matrices , the level of dissolved 62 and inoculum . the optimal ratio of hormones was got by uniform design according to its high immobilization level , high metabolism and retaining vigorous cells with long period . a medium which fit well all the conditions was obtained , and the efficiency of producing ginkgolides by cell culture improved obviously 進行了優良種系的誘導和優選,得到了一種生長快、分散性好,并很好的符合固定化要求的細胞株系;對固定化條件進行了最優化配置- -從載體預處理、載體種類、載體量、大小和溶氧到細胞接種量系統的研究;采用均勻設計尋求一種高固定化、高代謝及長期保持細胞活力的激素配比,最終得到一種基本滿足上述要求的培養基。

Biodiversity exists among am fungi and is influenced by numerous factors including soil properties and plant species . if am fungi are to be used in sustainable agricultural systems it is necessary to study native am fungi in the target areas and then select efficient isolates that can be applied as inocula in the field to improve crop growth . the objectives of this study were to investigate the germplasm of am fungi , to understand the distribution pattern of am fungi in different ecological conditions such as area , soil factor and host plant , to select isolates effective in nutrient acquisition by the host plant sweet potato , to test their effectiveness under field conditions , and to monitor amf after their introduction into the field 本研究通過調查我國北方部分地區的am真菌資源,研究了am真菌的種群組成及其在空間、土壤利用方式和宿主植物類型等不同環境條件和空間尺度上的分布規律;在此基礎上,根據它們對甘薯的生長、吸磷效應篩選出高效菌株,在大田條件下研究了am真菌菌絲的分布特性、代謝活性及其對甘薯產量和品質的影響;并通過分子探針跟蹤調查了引入am真菌在共生體中的發育和表達,以期為菌根真菌的生產應用提供技術支持。

Effects of physical factors on the growth of strain ag were of light intensity , temperature , inoculum volume , initial ph , effects of nutrient factors on the growth of strain ag included organic carbon sources , organic nitrogen sources , growth factors , hormone etc . , and the correlation among nutrient factors being considered . the growth condition of single filament on the plate was also studied 主要內容包括物理因子如光強、溫度、接種量和ph值等對螺旋藻生長的影響;化學因子包括有機碳源、有機氮源、生長因子、生長激素等對螺旋藻生長的影響及這些營養因子之間的綜合作用,并解決了單藻落平板生長問題。

The entomophthoralean fungus , pandora delphacis ( hori ) humber , ( zygomycotina : entomophthorales ) is an insect pathogen specific to homopteran insects such as planthoppers , leafhoppers , and aphids and frequently cause epizootics in insect populations . problems with propagation of inocula at a low cost , but high efficiency , have been a technical obstacle to progress for entomophthoralean study and utilization for a long run 飛虱蟲癘霉( pandoradelphacis ( hori ) humber )是同翅目昆蟲的專性昆蟲病原真菌,分類上屬于接合菌亞門( zygomycotina )的蟲霉目( entomophthorales ) ,常引發飛虱、葉蟬的流行病,也侵染蚜蟲、沫蟬等寄主。

The carbofuran - degrading experiment of cds - 1 was carried out in lab scale , the results showed that the highest degrading efficiency was obtained with ph , temperature being 7 . 0 , 30c respectively ; the change of aeration had no influece on degrading rate ; the increasing inocula could accelerate carbofuran degrading progress ; the degrading capability of cds - 1 was n ' t inhibited by high carbofuran concentration ; the addition of low concentration of nutrients had no distinct effect on the degrading rate while high concentration had inhibiting effect the distribution of degrading enzyme was also primarily studied , the results showed that degrading - related enzyme was endocellular and degrading progress was not cometabolism Cds - 1的降解酶(系)是誘導酶(系) ,存在明顯的誘導期;胞內、胞外酶實驗表明呋喃丹降解酶(系)存在于細胞內。添加低濃度外源營養物質對cds - 1的降解性能無明顯影響,說明cds - 1降解呋喃丹的過程不屬于共代謝過程,可以在無外源營養物質存在的條件下降解呋喃丹。添加高濃度外源營養物質會對該菌降解性能產生抑制。

Three different batches of broomcorn millet cultures obtained under uniform conditions in millet processing , initial inocula level , and incubation were found not significantly differing in sporulation capacity . on non - nutritional agar plates , temperature significantly affected sporulaiton 本研究用白碳黑、硅藻土和變色硅膠對新鮮黍米培養物進行處理并在5和10下保存,均導致其快速喪失產抱能力。

Effects of physical factors on the growth of strain ag were investigated , and suitable growth condition for strain ag were as follows : light intensity , 25001ux ; temperature , 30 ; inoculum volume , 10 % ( v / v ) ; ph , about 9 . 0 在研究物理因子對螺旋藻生長的影響的試驗中發現,光強2500lux 、溫度30 、接種量10 、 ph9 . 0左右時,有利于螺旋藻的生長。

Either instrument can be used to obtain the inoculum from an agar slant culture by carefully touching the surface of the solid medium in an area exhibiting growth so as not to gouge into the agar 任何一種器具都可以用來在洋菜斜面培養上獲得接種原,小心地碰觸有生長的固體培養基的表面,避免刺破洋菜培養基。

The swine manure was mixed with two kinds of anaerobic activated sludge at the mass ratio of 3 : 1 : 1 and the mixture was used as inoculum in the batch experiment 將豬糞與兩種厭氧污泥按質量比3 : 1 : 1混合作為接種物,試驗了纖維素、葡萄糖和乙酸作為磷酸鹽還原反應電子供體的可行性。

It is also more virulent because the inoculum required for septicum to cause an infection is ~ 300 times lower in mice compared with perfringens 小鼠實驗證實梭狀芽孢桿菌比產氣莢膜桿菌的毒性更強,因為前者造成感染的接種量僅為后者的約1 / 300 。

Initial ph and inoculum size were also investigated by one - factor experiments . the results were 6 . 5 , 10 % ~ 15 % respectively 通過單因素實驗得出最適初始ph為6 . 5 ,最適接種量為10 15 。

Note ? either absorbance or transmittance measurement may be used for preparing inocula 注:制備接種體的吸光度和透光率。

Effect of different inocula on anaerobic - thermophilic digestion of cattle manure 不同接種物對牛糞高溫厭氧發酵的影響

Carefully mix the inoculum and the medium 仔細混合接種物和培養基。

Sterile cotton-tipped applicators in individual glass tubes can be conveniently used to obtain inoculum during the necropsy . 裝在玻璃管中的滅菌棉簽適合干剖檢時用來采集接種物。