immunofluorescence n.【醫學】熒光免疫檢驗法。adj.-cent
n. 【醫學】熒光免疫檢驗法。 adj. -cent “anticomplement immunofluorescence“ 中文翻譯： 抗補體免疫螢光“antinuclear immunofluorescence“ 中文翻譯： 抗核免疫熒光“direct immunofluorescence“ 中文翻譯： 直接免疫熒光法,直接免疫熒光“immunofluorescence assay“ 中文翻譯： 免疫熒光測定法; 免疫熒光法“immunofluorescence method“ 中文翻譯： 免疫熒光法“immunofluorescence microscopy“ 中文翻譯： 免疫熒光顯微技術; 免疫熒光顯微術“immunofluorescence phenomenon“ 中文翻譯： 免疫熒光現象“immunofluorescence technic“ 中文翻譯： 免疫熒光技術“immunofluorescence technique“ 中文翻譯： 免疫熒光技術“immunofluorescence test“ 中文翻譯： 免疫熒光試驗“immunofluorescence testing“ 中文翻譯： 免疫熒光試驗“indirect immunofluorescence“ 中文翻譯： 間接免疫熒光法“kinetoplast immunofluorescence“ 中文翻譯： 動質免疫熒光“membrane immunofluorescence“ 中文翻譯： 膜免疫熒光“mixed immunofluorescence“ 中文翻譯： 混合免疫螢光法; 混合螢光免疫法“direct immunofluorescence technic“ 中文翻譯： 直接免疫熒光技術“direct immunofluorescence technique“ 中文翻譯： 直接免疫熒光技術“double-label immunofluorescence“ 中文翻譯： 免疫熒光雙重標記法“fixed-cell immunofluorescence“ 中文翻譯： 固定-細胞免疫熒光法“free viral immunofluorescence“ 中文翻譯： 游離簿免疫熒光“granulocyte immunofluorescence test“ 中文翻譯： 粒細胞免疫熒光試驗“ifa (immunofluorescence a ay)“ 中文翻譯： 免疫熒光測定（法）“ifa (immunofluorescence assay)“ 中文翻譯： 免疫熒光測定（法）“immunofluorescence antibody assay“ 中文翻譯： 免疫熒光抗體測定“immunofixation electrophoresis“ 中文翻譯： 免疫固定結合電泳,免疫結合電泳“immunofixation“ 中文翻譯： 免疫固定
immunogenetics |
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The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ) . the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd . the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus 結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限于細胞核內，而且隨著感染后( pi )時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉淀試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。 |
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Methods : the effects of different neurotrophic factors on the growth and differentiation of neural stem cells were observed by cells counting and immunofluorescence staining . the levels of rara mrna and rxra mrna in differentiated neural stem cells were assayed by rt - pcr . agarose gel electrophoresis and image analysis 方法應用細胞計數和免疫熒光細胞化學法，研究不同神經營養因子對神經干細胞增殖及分化的影響；應用rt - pcr 、瓊脂糖凝膠電泳和紫外分光圖象分析法檢測神經干細胞分化過程中rar和rxr mrna表達量的改變。結果1 |
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Subcellular localization by cell fractionation , immunofluorescence revealed that three human isoforms were associated with membranes involved in the autophagic pathway . the glycine and lysine respectively decided the location of autophagosome of human map1lc3a and map1lc3b . in addition , our results indicated that other proteins were involved in the post tranlational modification of human map1lc3b and this modication could be induced by amino acid starvation 人lc3a的膜定位與gly120位點的切割和修飾緊密相關，而人lc3b的膜定位是與lys122相關，進一步證明了修飾方式的特異性，對人lc3b的進一步研究表明參與其修飾的分子也與其它基因不同，并且饑餓可以誘導其修飾的發生。 |
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3 , the effect of k562 cell lines supernatant on no production by pbmc was investigated using the specific immunofluorescence probe daf - 2da . the results showed that the k562 cell lines supernatant could promote the no production by pbmc . further study found all the activated pbmc ( cd69 + ) produced no , while not all the no positive pbmc were activated 利用daf - 2da作為細胞內no特異性的熒光探針，研究了k562上清對pbmc產生no的影響，國內未見類似報導，發現腫瘤上清能促進pbmc細胞no的產生，進一步發現活化的細胞( cd69 + )均產生no ，而no陽性的細胞不一定活化的。 |
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4 . distribution and content of grp78 of hbmec in pre - invasion and post - invasion are of non - difference by means of immunofluorescence . while distribution and content of grp94 of hbmec in pre - invasion and post - invasion are of evident difference by means of immunofluorescence 4 .免疫熒光結果顯示，在正常的hbmec中， gr種8和g印四4的免疫反應陽性信號主要分布在細胞漿中，當大腸桿菌d抖侵襲hbmec后， grp78的含量和分布未見明顯變化，而gr即4的含量隨著侵襲時間的延長逐漸減少，并且其分布向胞膜附近轉移。 |
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Was first processed by dgd embedment and embedment - free technique and general technique for em morphology . a perinuclear structure consisted of interacted filaments we called lamina - like structure was observed . then using western blot assay , we found a lamin - like component band of 68kd protein in the three - step - fractionated cells . to investigate the distribution of the lamin - like protein in cells , a immunofluorescence experiment for in situ hybridization was designed using goat anti - lamin protein antibodies as the probes . the results revealed that the positive reactivity presented at different part of the cells . the perinuclear cross - actions were distinct , and cross - action with the oral apparatus and the cortex were also obtained 本文以dgd包埋去包埋技術對草履蟲的核纖層通過透射電鏡和免疫熒光分子雜交等技術進行了觀察。結果顯示，在核周存在由10nm纖維組成的核纖層免疫熒光結果表明，在核周呈陽性反應，并在其表皮口器等部位呈交叉的陽性反應蛋白分子雜交的反應帶在68kd處呈陽性反應。 |
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In order to investigate the role of mannose receptor ( mr ) of human sperm , the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography . the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope , respectively . the mice were immunized with pmr and the antiserum was raised . after capacitation and induction of the acrosome reaction , the human spermatozoa and oocytes were incubated with the antiserum . then the sperm penetration assay was undertaken 為了進一步探討人精于mr在精卵融合中的作用，本文采用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ，并將提純的人精子甘露糖受體( purifiedmannosereceptor , pmr )作用于去透明帶的金黃地鼠卵母細胞，運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素( tritc - lca )免疫熒光標記技術觀察pmr對卵子的影響。 |
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By nuclear transferee transfered the nih 3t3 cell to enucleated gv and mii oocyte . the immunofluorescence results showed that jak . 2 was not transfered to somatoblast nuclear whether the recombined oocyte occured gvbd or not . when transfered somatoblast nuclear to enucleated and integrated m ii oocyte , the immunofluorescence results showed that jak2 only existed in the nuclear of integrated m ii oocyte but not the somatoblast nuclear . the nuclear transfer implied that the mature and immature oocyte cytoplasm could not transfer the jak . 2 protein to donor nuclear 通過核移植，將nih3t3體細胞核移入去核gv期和m期的卵母細胞中。熒光檢測發現，體細胞核與去核gv期卵母細胞質構成的重組卵無論是否發生gvbd ，核內均沒有jak2的出現。在去核和未去核的m期卵母細胞中移入體細胞核， jak2僅存在于未去核m期卵母細胞的核內，并不轉移到體細胞核內。 |
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A rabbit was infected with a cloned yntatl , blood was collecting from from the rabbit every 3 days after infection within 30 days , 10 clonal trypanosome populations were gotten , infecting a new rabbit by the last non - cloned trypanosome population . repeated above all , thus infected 5 rabbits sequentially . twenty different vats ( variant antigen type ) were monitored and characterized from those fifty mono - clonal populations by indirect immunofluorescence test ( ift ) and avidin biotin enzyme immunoassay ( abc - eia ) 用伊氏錐蟲云南水牛單克隆株yntat1感染兔，感染后30天內，每3天從兔血中分離錐蟲并單蟲克隆，最后一個未單蟲克隆的蟲株感染另一只兔，重復以上操作，這樣順序感染5只兔子，共獲得50個單克隆錐蟲種群( tp ) ，經間接免疫熒光和abc酶標試驗鑒定共為20個抗原性互不相同的抗原變異體( vats ) 。 |
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But there are still no reports about the relationships of dnpi - like and gabaergic immunoreactive ( gad - li ) terminals with pag - like immunoreactive ( pag - li ) neurons in “ zone - shaped area “ . to answer these questions , we observed systemically the synaptic connections among the pv - like immunoreactive neurons , fibers and terminals and the connections between dnpi - like , gabaergic terminals and pag - li neurons using the methods of electron microscopic imrnunohistochemistry , triple - immunofluorescence histochemistry and retrograde tracing method combined with pre - embeded immunoelectron microscopic double - labeled technique 但是目前對新發現的囊泡膜mu轉運體一dnn樣陽性終末與帶狀區內pag樣陽性神經元之間ej關系，以及谷氨酸脫發酶（ gad ，是gaba能神經元和終末的特異性標識物）是否參與其調控作用，尚缺乏系統的形態學資料。 |
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After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells , the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope , indirect immunofluorescence and flow cytometer analysis . prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed 建系細胞培養上清與肝癌細胞作用后，經熒光顯微鏡觀察、間接免疫熒光及流式細胞儀檢測進一步確定表達的scfv融合蛋白具有與hbsag特異性結合的活性。 |
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The activity of the purified product was confirmed by indirect elisa analysis and was further confirmed by indirect immunofluorescence and immunohistochemistry after they were added to the culture medium of hepatocarcinoma cells 在大腸桿菌blzi中實現了scfv融合蛋白的表達。經ni nta柱純化獲得純化產物，經間接elisa分析確定所得產物具有與hbsag結合的特異性。 |
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6 . oocytes were fixed for immunofluorescence . examination of cgs and microtubules were performed by fitc labeled lens culinaris agglutinin ( lca ) and and - fi - tubulin under confocal scanning laser microscopy ( cslm ) respectively 利用直接免疫熒光染色和共聚焦顯微鏡（ confocalscan muglasermicroscopy ， cslm ） ，研究各組體夕成熟卵的皮質顆粒和微管 |
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If an immunofluorescence stain with antibody to complement or immunoglobulin is performed , then one can see the brightly fluorescing band along the dermal epidermal junction that indicates immune complex deposits are present 如果針對補體的抗體或免疫球蛋白進行免疫熒光染色,我們就能在表皮與真皮的交界處看到一條明亮熒光帶，表明有免疫復合物的存在 |
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We have identified p120ctn expression in cultured hepatocyte and liver tissues using rt - pcr , immunohistochemistry and immunofluorescence microscopy , p120ctn isoform 1a and 3 a expression have been detected in the cells and tissues 而當過度表達p120ctn后，細胞粘附能力有所增加，遷移能力降低，并且過度表達的pl加ctn主要分布于膜下，其次是胞漿。 |
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At high magnification , the dermis is expanded by dense collagenous fibrosis in a patient with systemic sclerosis . immunofluorescence is not helpful with scleroderma 高倍鏡下，系統性硬化癥病人真皮中膠原纖維增加使皮膚增厚。免疫熒光檢測對硬皮病是無用的。注意：近年來我們往往用系統性硬化癥取代名詞硬皮病。 |
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Subcellular localization by immunofluorescence revealed that two rat isoforms are associated with the autophagic pathway . these results are help to discover the mechanisms and functions of autophagy in vivo 另外，通過熒光定位以及與mdc的共定位分析發現兩個基因與大鼠的lc3相似，也是定位在自噬體上。 |
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The expression of scfvs fusion protein were detectable by fluorescence microscope directly and indirect immunofluorescence and immunohistochemistry analysis after transient expression in cos - 7 瞬時轉染cos刁細胞后，通過熒光顯微鏡觀察、間接免疫熒光檢測、免疫組化檢測證實了scfv融合蛋白的表達。 |
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Xu y , ding zr , su mq , et al . determining the origin of hematuria by immunofluorescence staining to erythrocytes in urine j . j fourth mil med univ , 2002 ; 23 ( 17 ) : 1596 徐焰，丁振若，蘇明權，等.免疫熒光細胞染色對腎性和非腎性血尿的鑒別j .第四軍醫大學學報, 2002 ; 23 ( 17 ) : 1596 |