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igy IGY = International Geophysi...

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The methological indices for the method were listed as follows : the detection limit or sensitivity was 1 . 67ng / ml ; intra - assay cv was 5 . 3 % , and inter - assay cv was 7 . 0 % ; the average recovery was 96 . 13 % ; the regression analysis was performed for the same sel of specimens ( 20 ) subjecled to bolh igg - ria and igy - ria , and results were quite well positively correlated ( r = 0 . 9988 ) 用本法與臨床實驗室應用的igg ria法同時測定20例標本后進行配對t檢驗,兩種方法測定結果差別無顯著性,呈顯著正相關( po 9988 ) 。本文制備的igy用于放免測定臨床標本能很好地區分甲亢、甲低和正常人群。

In this study , n - bulanol for defatted igy was superior to water dilution ( wd ) . after igy was defatted wilh n - butanol , further purification of igy wilh sodium sulphate and ammonium sulphate sailing out were compared . as a result , the salting out procedure of ammonium sulphale was superior to sodium sulphate 蛋黃正丁醇去脂后應用飽和硫酸銨和飽和硫酸鈉兩種不同鹽析法純化igy進行比較,飽和硫酸銨略好于飽和硫酸鈉鹽析法,其純化后所獲得的產量達9 . 5mgigy / ml卵黃,天津醫科大學碩士學位論文純度94

With anti - t - jgy establishing a radioimmunoassay , the quality of anti - t4 igy was appraised : litres was 1 : 25 00 , permitting rang of litres in the ria was from 1 : 2500 to 1 : 5000 ; the degree of cross reactivities with ta was 1 . 28 % ; affinity constants was 2 . 13 x 109l / mol 本文應用蛋黃正丁醇去脂獲得的抗t4 igy建立了放免檢測方法,對抗t4igy的質量進行鑒定:抗體滴度為1 : 2500 ,工作濃度在1 : 2500至1 : 5000范圍內;與t3的交叉反應為1 28 ;親和常數為2 13x109l m 。

Method was investigated to extract multiple proteins from complex system including water - soluble fraction of egg yolk and agkistrodon acutus venom by bacterial specific absorption . immunoglobulin yolk ( igy ) were absorbed directly from water - soluble fraction by streptococcus mutans , then eluted and further purified by columns of thiophilic gel . based on above experiments of antibody extraction , bacterium cells were applied further to extract interactive proteins from crude venom of agkistrodon acutus 首先,利用變異鏈球菌( s . mutans )與其特異性抗體的吸附作用從水溶性蛋白組分中分離出卵黃抗體( igy ) ,并將菌體上洗脫下來的igy用嗜硫色譜分離純化,得到高純度的細菌特異性抗體。

Different molecular weight igy exitence in the purified igy suggested that the igy is uniform . through colligate comparison , the best method suit for industrialization is eliminating lipid by 0 . 07 % alginic acid sodium combined with ammonium sulfate salting out afterl0 - fold diluted with ph5 . 0 water 通過綜合比較,認為酸性條件下10倍去離子水稀釋卵黃液,離心后上清用0 . 07的海藻酸鈉去脂,再用硫酸銨鹽析提取純化igy的工藝流程適用于工業化的規模生產。

One group was immunized with cdv vaccine , and the other group was cav . serum samples and eggs were collected weekly for detected antibody titers . high titer egg yolk was collected for following igy extraction 分別用cdv 、 cav的疫苗免疫產蛋雞,每周檢測雞血清和卵黃中的抗體水平,收集抗體水平高的雞蛋用于提取純化卵黃抗體igy 。

The result of elisa displayed that the all the serum against cdv and cav , and immunoglobulin against cav had high activity . but the igg against cdv did n ' t appear a high activity 結果表明狐抗cdv 、 cav高免血清和雞抗cdv 、 cav卵黃的活性都較高,但提純的抗cav的狐igg和雞igy的活性較高,而提純的抗cdv的狐igg和雞igy的效價較低。

No evenlt of the 1957 - 1958 international geophysicalrear ( igy ) exceeded in dramatlclmpac tand slgnl ficance theorbi lting of soviet satellite sputniki i in october 1957 譯為: 1957 - 1958國際地球物理年( igy )中的事件沒有一個能在其激動人心的影響和意義方面超過1957年10月蘇聯衛星“伴侶- 1號”進入軌道。

Test 3 : detected activity of serum and immunoglobulin samples by indirect elisa test , rabbit antibody against foxes ' igg and hen ' s labeled with hrp igy was used in indirect elisa test 試驗三:間接elisa檢測的抗體活性。用過氧化物酶標記的兔抗狐igg和雞igy抗體進行間接elisa測定獲得的樣品的活性。

The result of sds - page certificated the method 6 % octanoic acid combined with ammonium sulfate got the highest purity , the next was alginic acid sodium combined ammonium sulfate Sds - page和低壓層析的結果證明,用6辛酸?硫酸銨法提取igy的純度最好,其次是海藻酸鈉?硫酸銨法。

Igy was extracted from diluted egg yolk with different fold water , and 10 - fold dilution with phs . o water was manifested the best way that lipids can be eliminated from the supernatant 驗證了卵黃液用ph5 . 0的10倍去離子水稀釋后的去脂率和igy提取產量要高于其他稀釋方法。

Except the igy got from by the method 6 % octanoic acid combined with ammonium sulfate , there were over two tape in others sds - page electrophoretogram 獲得igy產量以海藻酸鈉?硫酸銨法最高,可達5 . 69mg ml ,其次是用3辛酸?硫酸銨和聚乙二醇法。

In general , n - butanol for defatted igy is a simple , rapid and efficient procedure 總之正丁醇去脂是一種快速、簡便、有效的純化igy的方法。

The rate of chromosome non - disjunction was higher than that of control group from 0 Igy開始引起染色體不分離率較對照增加(尸o

Then 7 different methods for extracting igy were compared 然后在此基礎上比較了7種不同提取卵黃抗體的方法。