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icr ICR = Institute for Cancer R...

icrc

20 students from scnu served as the subjects , and they were randomly divided into the hyperoxia group and the control group . the venous samples were taken before exercise , just after exercise , post - exercise somin and 24h , respectively . the immune parameters included rbc - csbrr , rbc - icr , immunoglobulins ( igg , iga and igm ) 將實驗對象分為吸氧組和對照組,測得兩組安靜時、運動即刻、恢復30分鐘和恢復24小時的免疫指標的變化,包括紅細胞免疫功能指標,即紅細胞補體受體花環率( rbc - c _ ( 3b ) rr )和紅細胞免疫復合物花環率( rbc - icr ) ;免疫球蛋白igg 、 iga 、 igm ,并對上述指標的變化趨勢作了分析。

Pulmonary fibrosis was induced in the model group by intranasal instillation of bleomycin 5 mg kg . hxd were given via gastrogavage to the hxd prevention group and treatment group respectively at 48 h before and 14 days after they were modeled with the same method , and the groups of ligustrazin and prednisone , treatment and prevention , were managed the same as for hxd groups , but with ligustazin and prednisone instead of hxd respectively . bronchoalveolar lavage fluid balf and lung tissue of animals were gathered on day 28 cellular ultrastructural changes of lung was examined under electron microscope by he , masson stain ; content of hydroxyproline in lung tissue was assessed by acidolysis method ; and concentration of transforming growth factor 1 tgf 1 in balf was assessed by elisa 方法68周雄性icr小鼠分8組,模型組bm組經鼻滴入博萊霉素blm 5 mg kg建立肺間質纖維化模型,化纖湯預防治療組pht組于滴入blm前48 h予化纖湯灌胃,化纖湯治療組ht組于滴入blm后第14天用化纖湯灌胃,同時設立丹參預防治療組pdst組及丹參治療組dst組,地塞米松預防治療組pdt組及地塞米松治療組dt組,并與正常小鼠nc組進行對照。

Embryonic stem ( es ) cells were derived from the inner cell mass ( icm ) of preimplantation embryos , and embryonic germ ( eg ) cells from primordial germ cells ( pgc ) . both es and eg cells were cultured to determine the factors affecting on isolation , cloning and passage of pluripotent stem cells from icr mouse embryos 試驗以icr小鼠胚胎內細胞團( icm )和原始生殖細胞( pgc )為材料,采用不同培養體系分離克隆胚胎多能干細胞,探討icr品系小鼠胚胎多能干細胞分離克隆的基本方法,以及一些因素對其分離、培養、克隆、傳代等效果的影響。

Compared with control group , rbc - cabrr in hyperoxia group showed more significant increase and rbc - icr more significant decrease . , up to 24 hours later , even lower than pre - exercise level . it suggested that hyperoxia intake , to some extent , may accelerate the recovery rate of immunosuppression after acute exercise , and that hyperoxia intake can serve as a method to eliminate exercise - induced fatigue ? ? df ? ?主要反映在紅細胞免疫功能的變化,吸氧組rbc c3brr在恢復30分鐘時上升更明顯,同時icr下降也更明顯(吸氧組p隊05 ,對照組p o0引,且恢復n小時下降至安靜值以下。

We used the icr and kunming mice and got the embryos of 3 . 5dpc by means of superovulation , then cultured the embryos on the feeder layer derived from icr or kunming mice , dispersed the inner cell masses ( icms ) or es cell colony in the appropriate time . in this period , we analized the effects of feeder layer , trypsin and embryos isolated from uteri of different varietal mice on the es cell lines 以icr小鼠和昆明小鼠為實驗對象,采用超數排卵的方法獲得小鼠胚胎,培養在用絲裂霉素c處理過的icr小鼠和昆明小鼠mef細胞飼養層上,選取適當時間離散增殖的icm和類es細胞集落,分析了在小鼠es細胞建系過程中,飼養層細胞、消化液及不同品系來源的胚胎的影響。

Mouse blastocysts and morulas were cultured in rat heart conditioned medium ( rh - cm ) . as far as the following indexes attaching ratio , icm growth ratio and es clone formation ratio are concerned , the choice of blastocysts is much better than morulas ( p < 0 . 05 ) , therefore mouse blastocysts were more suitable than morulas to get es cells 源于icr小鼠icm的es細胞分離克隆研究試驗以rh - cm為培養基培養處于不同發育階段的小鼠胚胎,發現由囊胚分離的es細胞傳代后es集落的出現率顯著高于桑椹胚( p 0 . 05 ) ,故囊胚更適合作為es細胞分離克隆的材料。

In the laboratory experiment part , human peripheral blood , cultured cells and icr mice were study objects . the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test . the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test . the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry , western blot and rt - pcr 流行病學結果顯示長期小劑量輻射接觸與染色體不分離呈正相關,為進一步在細胞遺傳學和分子生物學方面研究小劑量電離輻射與染色體不分離關系及其機制,本課題第二部分以外周血、培養細胞、 icr小鼠為研究對象,用外周血染色體計數和單細胞受精卵染色體計數的方法研究小劑量輻射和拓撲異構酶復旦大學2000級博士生學位論文11a抑制劑及其二者的協同效應對有絲分裂和減數分裂染色體不分離的影響,用免疫細胞化學染色、 westernblot 、 rt pcr等方法研究了電離輻射引起拓撲異構酶a表達變化。

The results showed that the feeder layers prepared by icr and kunming mice had no significant effect on adherence of embryos and proliferation of icms . the trypsin , which affected the isolation of es cells remarkedly , should be used with concentration of 0 . 125 % trypsin + 0 . 02 % edta for kmsmin 從實驗結果來看, icr小鼠和昆明小鼠兩種來源的飼養層細胞對胚胎貼壁、 icm增殖無影響,消化液作用影響明顯,以0 . 125 trypsin + 0 . 02 edta消化10 15min為宜。

Ocular injury to icr mice from embryonated eggs of toxocara canis , which had been maintained in 2 % formalin for 14 months at 4 c , was evaluated by microscopic and pathologic assessments at 1 , 2 , 3 , 5 , 7 , 28 , 56 , 84 , 112 , 140 , 168 , 196 , 294 and 469 days post infection ( dpi ) 摘要本研究探討將儲存于2 %福馬林液內,于4 c下保存14個月之犬蛔蟲受孕卵感染小鼠第一天至第四百六十九天后,藉顯微鏡檢視與病理學的研究評估孵化的幼蟲對小鼠的眼部損傷情形。

Otherwise , rbc - icr showed a continuous fall somin and 24 hours after exercise , and returned to pre - exercise level 24 hours later . it indicated that the erythrocyte immune function showed an enhanced state somin after exercise and had basically restored 24 hours after exercise 急性遞增負荷運動恢復期機體免疫機能的變化規律如下:運動后恢復30分鐘時,紅細胞免疫功能增強,免疫抑制解除,紅細胞免疫粘附活性高于安靜值, 24小時后恢復正常。

2 . isolation and cloning of mouse embryonic germ cells of icr species primordial germ cells ( pgc ) were isolation from 8 . 5 - 15 . 5 dpc ( days post coitum ) embryos . eg cell lines with the characteristic of murine es cell line were established and continuously cultured to 6th passage Icr小鼠eg細胞的分離克隆研究本試驗以icr品系小鼠胚胎8 . 5 15 . 5dpcpgc為材料,經傳代培養,獲得能連續而穩定傳至6代的,具有胚胎干細胞諸多特性的eg細胞系。

The purpose of this study was to establish es or eg cells lines in the future for further research . the results attained were as follows : 1 . derivation of embryonic stem cells from icr mouse preimplantation embryos 本試驗對icr小鼠胚胎多能干細胞的建系工作做了初步探索,為胚胎多能干細胞多種屬廣泛建系的研究打下一定基礎,也為小鼠胚胎多能干細胞的深入研究積累經驗并提供借鑒。

In the experiment , in case of the proliferative capability of eg cells , the composition of 0 . 25 % trypsin + 0 . 04edta was optimal . the growth behavior of icr murine eg cells from pgc was observed 傳代過程中,以0 . 25胰酶與0 . 04 edta聯合作用效果較好,對eg細胞的綜合損傷最小,離散傳代后eg細胞活力比較強, eg克隆出現最多,最高傳至6代。

Numerous editing functions allow total flexibility when working with recorded files . insert , overwrite , erase and partial erase included in the functionality of the icr - b35 當眾多的編輯功能,讓整體工作的靈活性錄的檔案.插入、改寫、抹去抹去,包括在部分功能的的icr - b35

Furthermore , it is more easy to isolate embryos and get the es cells from icr mouse than kunming mouse 以上結果表明: icr小鼠比昆明小鼠更易進行es細胞的建系工作。

Fouier transform ion cyclotron resonance mass spertrometer ft - icr - ms 傅立葉變換離子回旋共振質譜計

Primary culture and identification of icr mice sertoli cells 小鼠睪丸支持細胞的原代培養和鑒定

An icr - based spread spectrum communication with arbitrary rate 的任意傳輸速率擴頻通信系統

Ion cyclotron resonance mass spectrometer ; icr mass spectrometer 離子回旋共振質譜計