H - hybridize 中文

hybridize vi.,vt.(使)產生雜種，(使)雜交；(使)混成。

hybris
The work on physical mapping of the chromosome of s . nanchangensis ns3226 was initiated . nearly a full set of chromosomal asei - bamhi fragments of s . nanchangensis ns3226 were cloned and used as probe to hybridized against its genomic library . thirty four asei linking cosmids were observed from 162 hybridizing cosmids and 20 of them showed no obvious overlapping each other by bamhi digestion , suggesting distinct identifications 此外，還開展了南昌鏈霉菌ns3226染色體物理圖譜構建的前期研究工作：基本克隆到了南昌鏈霉菌ns3226染色體上全套的ase - bamh片段，以它們為探針從南昌鏈霉菌ns3226的基因文庫中釣到164個陽性克隆，并從中篩選到34個ase linkingcosmids ，用bamh進行初步的酶譜分析，結果表明其中有20個cosmids的bamh酶譜相互間沒有明顯的重疊性。
Although bt cotton altogether can increase yield by 9 . 6 % , the yield performance of bt cottons hybridized by monsanto or caas genes acquired from informal channels with local cotton varieties is 5 . 6 % less than that of caas and monsanto formal seeds , though it ' s yield is 1 . 4 % higher than that of non - bt cottons . bt cottonseeds with different quality have different impacts on yield . the high quality cottonseeds can increase cotton yield 14 % more than inferior cottonseeds 雖然bt抗蟲棉總體可以提高棉花單產9 . 6 ，但是通過非正式渠道獲得中國農科院( caas )和孟山都( monsanto ) bt基因并與當地品種復交后的不能確定基因來源的抗蟲棉種子產量表現比caas和monsanto的正規種子至少要低5 . 6 (雖然比常規的棉花品種還是高1 . 4 ) 。
We found that sit variant gene ( slt2vha ) was identified in strains e . coli o157 : h7 isolated from patients and dung beetles 2000 in xuzhou city , jiangsu province . the primers used for stx2 variant analysis are shown in tablel . genomic dna restriction fragments digested by pstiwere sonthern - blotted and hybridized with an stx2 - specific dna probe . the probe was prepared fromed a 285 - bp pcr productof the strain 882364 stx2 gene obtained by using the specific primer pair ( tablel ) 2000年在江蘇省徐州市銅山縣腹瀉病患者的糞便標本分離的10株產生志賀毒素的菌株以及從蜣螂腸道分離到的4株產生志賀毒素的大腸桿菌o157 ： h7 ，屬于另兩個pfge型，和1986 、 1987 、 1988年在徐州市腹瀉病患者的糞便標本分離的菌株pfge圖譜不同。
Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months . the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg , and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa . through three clonings less diversed anti - p24 hybridoma cells are gained 方法：基因工程p24抗原免疫小鼠4次，歷時2個月，取脾細胞與骨髓瘤細胞株sp2 0 ，用peg融合， hat選擇培養和間接elisa篩選分泌抗p24抗體陽性的雜交瘤細胞，三次克隆化后得穩定分泌抗p24抗體的雜交瘤細胞株。
To dress the question if other virulence gene were present in this kind of strains , 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study . the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e . coli ( etec ) , invasive plasmid antigen b ( ipab ) for enteroinvasive e . coli ( eiec ) , epec adherence factor ( eaf ) , epec secretion protein c ( espc ) for enteropathogenic e . coli ( epec ) , hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e . coli ( ehec ) and eaggec probe for entero - aggregative e . coli ( eaggec ) . the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e . coli ( upec ) and “ o “ island 28 ( rtx 615 ) gene was also detected , the later was a newly discovered putative pathogenicity island in e . coli o157 : h7 為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因，選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株，進行在致瀉性大腸桿菌的25個毒力基因的檢測，包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ，腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ，腸致病性大腸桿菌的eaf 、 espc基因，腸出血性大腸桿菌的溶血素hly 、志賀毒素1 ( slt1 ) 、志賀毒素2 ( slt2 )基因，腸集聚性大腸桿菌的eaggec探針，以及在泌尿道致病性大腸桿菌和o157 ： h7大腸桿菌中新發現的毒力島基因。
The results showed that nb14 and ny 13 had positive hybridization signals , but no signal in the parent - common wheat , and nb14 fragment only had positive signal hybridized with line24 . therefore , we can not deduced it is a responsive gene at present . much work still needed to confirm it 繼續用ny13片段為探針，進行southern雜交分析，結果只與多枝賴草有雜交信號，且信號強，進一步證實該est是多枝賴草總基因組dna本身所具有的特異序列，雜交片段本身很可能是多枝賴草六側協迫應答新基因之一或其一部分。
The ph ultra - smal1 biosensor was successfuly used to detect ph value in singie cell . wr biosensors for proten and glucose were also proposed . ped one : in chapter l , three ams were synthsized to find a suitable probe to hybridize the expression of ing1 gene , a recentiy identified tumor suppressor gene 并且，將能表達ing1基因的質粒轉入腫瘤細胞進行培養后，再將分子信標轉入腫瘤細胞，發現轉導了質粒的腫瘤細胞比沒轉導質粒的腫瘤細胞內的熒光明顯增強，其增強程度可用光譜掃描的方式將其量化。
Total cellular rna were extracted , 40 micrograms of the total rna were used in the reverse transcription reaction , using superscript ii reverse transcriptase , oligo ( dt ) i8 primers , and cy3 - dctp or cy5 - dctp for the experimental and control group respectively . the labeled cdnas were hybridized to microarrays at 42c for 12 h - 18 h 提取as _ 2o _ 3作用k562細胞前后的總rna ，用superscript逆轉錄酶逆轉錄成cdna第一鏈，并在逆轉錄的過程中，用cy3 cy5熒光染料分別標記對照組處理組，與自制的k562細胞基因表達譜芯片雜交。
According to marketing segmentation theory and positional theory , the paper considers the corporation ’ s ability and resource and chooses the professional hybridized pig culturist and scattered hybridized pig culturist . after making a market research and gaining the data and using the joint - analytical method of orthogonal design method to calculate the product preference of market culturist , then comparing the market position of competitor , the paper ensures kangda corporation ’ s product position to supply the differential marketing strategy 在此分析基礎上,本文根據市場細分和定位理論,結合公司的能力和資源,評估和選擇了土雜豬專業戶和土雜豬散戶這兩個具有發展潛力的細分市場,并根據市場調查獲得的數據,采用基于正交設計的聯合分析法分別獲得了目標市場養殖戶購買偏好,結合競爭對手的市場定位,確定了公司市場定位,為后續的差異化營銷策略制定提供支撐。
Ny13 fragment had positive signals hybridized with line 14 and leymus multicaulis , which proved that it was a responsive gene in salt stress . furthermore , we tested ny 13 with southern blotting , and also found positive signal in the leymus muhicaulis . this segment may be correlated with the addition chromosome and has the character of salt - resistance 繼續回收雜交信號處的ona片段，連接到可轉化人l _染色體( tac )載體上，構建成含有多枝賴草鹽脅迫應答基因的tac克隆，以期今后通過耐鹽功能互補實驗驗證而直接用于基因_程育種，井可進一步篩選分離獲得耐鹽相關基因。
Abstract : according to the characteristic of hybridize and sudden change of binary coding chromosome in classical genetic algorithm , this paper defines the rule of hybridize and sudden change of continuous variables . the method is noted for easy to operate and quite good to approach the best true solution in overall situation . by means of three computed examples of nonlinear optimization and quadratic programme problem , it confirms that the method has strong adaptability and can precisely determine the best solution , in overall situation 文摘:根據傳統遺傳算法中對于二進制編碼染色體的雜交和突變的特點，定義了連續變量的雜交、突變規則.此方法有操作簡便，可較好地逼近全局最優的真實解的特點.通過非線性優化和二次規劃問題的3個算例，證實本算法適應性強，可較精確地確定全局最優解
The loop sequence of mb1 and mb2 were the anti sense and sense sequence ofing1 , respectively the sequence of mb3 was a piece of ssrna sequence in tobacco mosaic virus , which had no analogical to human gene . mbl was the most suitable probe because mbl had the highest fluorescence enhancemen after hybridizing wtth rna extrated froin normal cell 第三章，根據一種常見的病毒煙草花葉病毒( tmv )的核酸序列設計了分子信標熒光探針，由于tmv的遺傳物質是rna ，分子信標又具有很高的特異性和靈敏度，因此感染了病毒粒子的植物葉片在經過簡單處理后，可用分子信標檢測葉片上。
Finally , genetic optimization research is summarized on several typical production scheduling problems . after expounding the general idea of genetic algorithm , the comparative advantages in contrast to the traditional algorithm , the basic characteristics of genetic algorithm and its theoretical base , the paper puts emphasis on the efficiency of genetic algorithm in the scheduling of flow shop , and puts forward an improving genetic algorithm : the ordinal genetic algorithm based on the heuristic rules . the new algorithm introduces into the initial group the solution of heuristic algorithm , and in the group structure adopts a strategy of first ordering according to the priority of the adaptive solution , and then defining a new way of choosing probability by segments , which provides more hybridizing opportunity for optimized individuals , and designs variation - control rule to prevent single population and partial optimal solution 在論述了遺傳算法的思想、與傳統搜索算法的比較優勢、遺傳算法的基本特征和遺傳算法的理論基礎(包括模式定理、隱含并行性、基因塊假設、欺騙問題和收斂性定理)后，重點探討了遺傳算法在flowshop調度問題中的潛力和有效性；結合啟發式規則，提出了一個改進的遺傳算法?基于啟發式規則的有序遺傳算法，新算法在初始種群中引入了啟發式算法的解，在種群結構上采用了先按適應值優劣排序再分段確定選擇概率的新策略，使優質個體有更多的雜交機會，在變異中設計了變異控制規則，以防種群單一化，而陷入局部優化解。
Pfge analysis of these 21 blocked strains revealed a common chromosomal deletion in the 300kb asel fragment which might be responsible for antibiotic 5102 - iii biosynthesis . so the 300kb fragment was recovered and used as probe to hybridize with 10 - 22 genomic library . cosmids in this region were aligned and suitable fragments in this region were selected and used further for the construction of gene replacement plasmids 以此片段為探針釣出了位于該區域的文庫克隆，并利用指紋印跡和雜交技術將這些文庫克隆排列起來，進而以位于該區域的不同位置的片段做臂構建了用于轉化和接合轉移用的基因置換質粒，并試圖通過基因置換將該區域置換下來，但尚未得到最終結果。
It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent , genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study , genetic diversity in a . polytricha was higher than that in a . auricula : 4 in this study , a . fuscosuccinea had a higher homology to a . auricula than to a . polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a . auricular and a . fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a . auricula as a probe which hybridized with a . auricula and a . fuscosuccinea except a . polytricha , further confirming the veracity of the results from eric - pcr ; 7 in this study , isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity 本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用于木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
Besides , to discuss the methods and pathways for building the hybridize parents of excellent quality by yunnan scent soft rice germplasms and for selection breeding the hybrid rice of high quality , to discuss the scent gene genetic expression of yunnan rice germplasms and the problem of breeding application 此外，討論利用云南香型軟米稻種資源創建品質優異雜交稻親本和選育優質雜種稻的方法和途徑，以及云南稻種資源香味基因遺傳表現及其在育種中應用問題。
Experiments of nucleic acid hybridization show that taqman 6 only hybridizes to the pcr products amplified from s . costatum , but not to products from other microalga . these results indicate that primer6 ( f / r ) and taqman6 can be used to establish the rfq - pcr method for the quantitative detection of s . costatum 最后以primer6rdna序列在幾種浮游植物的分類及中肋骨條藻定量檢測中的應用( f / r )和taqman6探針建立了定量檢測中肋骨條藻的rfq一pcr 。
Differential hybridization of human testis cdna micorarrays human testis cdna microarrays were constructed by spotted the pcr product amplified from human testis library . then membranes were hybridized using the probes of embryonic testis ( 6 months ) and adult testis 人睪丸cdna微陣列雜交從人睪丸文庫中pcr擴增插入片段，將pcr產物點膜制備成cdna微陣列，然后提取胚胎和成人睪丸的mrna ，制備成探針，分別與微陣列進行雜交，獲取差異表達克隆。
Sh - sy5y cells were differentiated into neuron - like cells by ra treatment in low concentration , total rna was extracted and labeled to hybridize with the microarray , the changes on gene expression were detected to find differentially expressed genes 首先應用低濃度的ra處理sh - sy5y細胞，促使細胞分化成神經元表型，然后提取總rna并進行標記，與制備的基因表達譜芯片進行雜交，檢測基因表達變化，查找差異表達基因。

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hybridize

hybridize vi.,vt.(使)產生雜種，(使)雜交；(使)混成。

hybris