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uploads/hematoxylin.jpg

hematoxylin n.= haematoxylin.

hematozoon

Section two the evaluation of biocompatibility of the acellular dermal matrix by the method of cell culture . the new born rat ' s epdermic cells were cultured with the acellular dermal matrix together as experiment group , while the epdermic cell were cultured simply as control . 24 hours later , under the invert microscope , the epidermic cells anchored well and transparent flat cells were observed in both groups . 7 days later , both cultured cells were taked out and fixed in 95 % ethanol , stained with hematoxylin and were observed under light microscope . many cleaved cells were observed in both groups . during cell culture , no pathogenic microganism was observed . so we considered the acellular dermal matrix was aseptic and had good biocompatibility . section three subdermal implantation of the acellular dermal matrix . 24 rats were used in the experiments . a piece of acellular dermal matrix ( 1 . 5 x 1 . 5cm2 ) was implanted beneath the dorsum skin flaps of each rat , 1 week , 2 weeks , 3 weeks and 4 weeks after implantation , 6 pieces of acellular dermal matrix were harvested and the size of implanted acellular dermal matrix were measured , the sections were used for he staining and observed under light microscope . the result were as folio wing : 1 - 2 weeks after implantation , the acellular dermal matrix began to adhere to the tissue around and turned red gradually ; 3 - 4weeks after implantation , the acellular dermal matrix adhered closely to the tissue around and could be recognized easily , 1 - 3 weeks after implantation , the size of implanted acellular dermal matrix had no statistical difference ( p > 0 . 05 ) . 4 weeks after implantation implanted acellular dermal matrix contracted ( p < 0 . 05 ) . under light microscope , l - 2weeks after implantation , the fibroblast cells infiltrated the acellular dermal matrix and a small amount endothelial cells of vessel and lympho - histiocytic cells infiltrated the acellular dermal matrix . 3 - 4 weeks after implantation , infiltrating blood vessels were evident . so we think that the acellular dermal matrix had low immunological reactions and could induce the infiltration of fibroblast macrophage cell and the endothelial cells of vessel 結果如下:皮下包埋卜周者,無細胞真皮基質漸與周圍組織粘附,顏色由蒼白轉紅;皮下包埋3周者,無細胞真皮基質與周圍組織緊密枯附,盾晰葉辯;術后卜周,包埋的基質面積變化較包埋前無統計學差異o川0引,術后4周包埋的無細胞真皮基質面積較包埋前縮小j刃刀5 ) 。光鏡下術后卜周,宿主的淋巳組織細胞、成纖維細胞浸入生長,釉附在膠原纖維上,少量血管內皮細胞浸入基質;術后34周,無細胞真皮基質內較多的血管形成,故可認為無細胞真皮基質免疫原性低,能誘導宿主的成纖維細胞、巨噬細胞浸入生長,為一種新型的真皮替代物。第四部分無細胞真皮基質與自體斷層皮片復合移棺的研究, sd大鼠10只,在其背部卜方造成全厚皮膚缺損的創面

The early embryo were made into a series of continuous section slides by tissue cutting . the sections were stained by hematoxylin and eosin ( h & e ) staining and then the development of internal organs such as heart in early embryos was observed by microscope . we found that there is certain relationship between external and internal malformation 同時我們收集人類藥物流產的早期胚胎,觀察發現胚胎畸形占17 . 86 % ,早期致死占32 . 54 % ;采用組織切片技術將胚胎制成一系列石蠟連續切片,染色后顯微觀察畸形和正常的早期胚胎內部心臟等器官的發育情況,發現胚胎外部畸形與體內畸形存在一定關聯,對此我們將做進一步的研究。

In this paper , the methods that the author used are as follows : light microscopy : the testis was fixed in bouin ' s fluid , dehydrated in an ethyl alcohol series , embedded in paraffin , sectioned at 6 u m and stained with hematoxylin and eosin , then observed with olympus microscopy and photographed 光鏡樣品以bouin ' s固定液固定,系列酒精脫水,石蠟包埋,切片厚度6 m ,蘇木精、伊紅染色, olympus顯微鏡觀察并拍照。

In order to understand the mechanism of spermatozoa living in the spermatheca after copulation , hematoxylin - eosin dyeing method is used to discover the microstructure of the spermatheca by light microscope 為進一步了解雌雄個體交配后精子在受精囊內的存活的機制,采用h - e法,在光鏡水平上研究了受精囊的顯微結構。

Finally , the sections were counterstained with hematoxylin , dehydrated and cover - slipped . statistical analysis the data analysis were performed using spss for windows 10 法醫病理學上關于icam ?一的研究已有很多,主要集中在應用icamj判定心肌缺血及腦損傷時間可行性方面。

Cells take diverse shapes . these are epithelial cords of block - like cells . as always , nucleoli and nuclear heterochromatin stain darkly with hematoxylin 不同形態的細胞。這些是立方細胞排列成的上皮索狀結構。總之,核仁與細胞核中的異染色質被蘇木精染成深色。

Elimination of hematoxylin sediments in he sections 切片中蘇木素沉渣去除方法探討