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hemagglutinin n.【醫學】血球凝集素。

hemal

The fusion ( f ) protein and hemagglutinin - neuraminidase ( hn ) are the major virulence factors of the virus to which the host responds well with protective antibody production . recombinant plasmids containing the f or hn gene can induce specific immune responses against ndv . however , dna vaccination by injection or gene gun routes is rather inconvenient and costly in field applications in scaled animal farms 融合蛋白( f )和血凝素-神經氨酸酶蛋白( hn )是ndv的主要宿主保護性抗原,以f基因和hn基因為免疫原研制的dna疫苗能誘導一定程度的免疫應答,但注射免疫或基因槍免疫途徑因實際操作和成本等問題難以推廣應用,而直接口服重組質粒dna免疫效果不理想。

A surface antigen hemagglutinin - neuraminidase ( hn ) gene of the ndv b95 strain , was selected to study as target gene . referred to the reported sequence , rwo primers were designed and synthesized . hn gene of ndv b95 strain was amplified by rt - pcr , the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fraction method 為此,從澳大利亞某大學獸醫病理系引進一株ndv熱穩定性天然弱毒b95株,并選取其表面抗原血凝素?神經氨酸酶的編碼基因為目的基因,根據國外已發表的序列,設計一對引物,利用rt - pcr技術擴增出了ndvb95株hn基因。

To elucidate the antigenic drift and evolution of h9n2 subtype avian influenza viruses ( aivs ) , five isolates from the north of henan province during 1998 - 2002 were compared and analysed by cross - hemagglutinin inhibition test ( hi ) , cross - virus neutralization test ( vn ) in the chicken embryo and chicken embryo primary kidney cell ( cek ) and cross protection against challenge infection test 為了探討h _ 9n _ 2亞型禽流感病毒的抗原性有否發生漂移,本研究從生物學角度和ha基因分子水平上對1998 ? 2002年間在河南省豫北地區分離到的5株h _ 9n _ 2亞型禽流感病毒的抗原性變化進行了比較和分析。

Hemagglutinins inhibition ( hi ) testes showed that there were obvious variations among the isolates from different geographical areas . this result explained why the protection rates of current vaccine were different around the country . dna sequence analysis of h9 hemagglutinin genes showed that these viruses were closely related and possibly came from one source 且在同一地域內,不同的異變體之間的差異較小,隨著時間的推移,其變異并不明顯;但在不同的區域內分離的毒株之間變異較為突出,這表明中國大陸h9n2亞型禽流感病毒不同分離株抗原性是與地域的改變而發生較為明顯的變異。

In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed , hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3 應用反轉錄-聚合酶鏈反應( rt - pcr )獲得兩株新城疫病毒( ndv )血凝素-神經氨酸酶( hn )基因cdna ,然后定向插入真核表達質粒pcdna3中,構建了含hn基因的重組質粒。

Hemagglutinin ( ha ) is proved to be the main immune pathogen of chicken accounting for inducing of antibody against ai and main target antigen causing humoral immunity in chicken . it plays an important role in anti - infection immunity 研究表明其ha基因是誘導機體產生抗體的主要免疫原,是機體對禽流感病毒產生體液免疫的主要靶抗原,在機體的抗感染免疫中起重要作用。

Abstract : this paper describes the role of the sialylsugar chains on cell surface in the attacking process of the influenza virus , and also binding specificity of hemagglutinin of the influenza virus to the sialylsugar chains 文摘:介紹了細胞外表面上含唾液酸的糖鏈在流感病毒侵襲細胞中的作用以及糖鏈與流感病毒表面糖蛋白-血凝素結合的特異性。

The a / pr / 8 / 34 ( hln1 ) adapted to mouse lung was propagated in allantoic cavity of 9 - day - old embryonated chicken eggs and titered by hemagglutinin test , and the tilers was 1 : 320 用9日齡雞胚培養人流感病毒的鼠肺適應株戶jp側8 / 34 ( hini ) ,通過血凝實驗測得病毒滴度為1 : 320 。

Based on the published aiv h7 ha1 cdna sequences , a pair of primers were designed which were specific to hemagglutinin ( ha1 ) genes of h7 subtypes 根據已發表的aivh7ha基因序列,設計了針對ha1基因的一對引物,經rt - pcr方法擴增出了約1 . 0kb的基因片段。

The difference of the hemagglutinin of h9n2 chicken influenza viruses isolated in china and korea 2病毒血凝素分子特性的區別

Cloning and sequecing analyzing of the hemagglutinin gene from influenza a h3n 基因真核表達載體的構建及序列分析

Analysis of hemagglutinin genes of two avian influenza virus isolates 2亞型流感病毒的發現及感染人調查

Characterization of hemagglutinin gene of influenza a virus subtype h9n 毒株基因組特性的研究