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hemagglutinate vt.【醫學】使血球凝集。n.-nation

hemagglutinin

The change of agglutinating activity , cd spectrum and fls of lra in different temperature , ph and different chemicals indicated that lra had partial hemagglutinating activity at ph2 . 0 ( 50 % ) , a temperature above 100 ( 60 % ) and after modified by n - bromosuccinimide ( mbs ) , the activity lost completely , modified by depc , the lra had a little activity , the other groups modified such as arg , tyr , glu , asp did n ' t effect the hemagglutinating activity of lra . the result indicated that trp residues were essential to the hemagglutinating activity and were involved in carbohydrate - binding site 研究不同溫度、 ph和基團特異性化學修飾后lra凝血活性和促淋巴細胞有絲分裂的變化、圓二色譜和熒光光譜的變化,當溫度達80以上時,活性開始下降,到100時活性有60 %保留:當ph為2時,活性保留50 % , ph為4一12對活性的影響不大;用nbs修飾trp后, t即的旦一叫睬基的破壞使活性完全喪失,表明trp對凝血活性是至關重要的, arg 、 tyr 、 glu 、 asp被修飾后, lra的凝血活性并未受到大的影響,但tyr修飾后lra的促有絲分裂活性降低

The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times , gross lesions were observed in chicken embryo . the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin . to surprise , the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells . in addition , the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion , but it ' s gross lesion did n ' t resemble to those of field outbreaks 生物學特性:雞胚尿囊液經離心、磷鎢酸負染后,電鏡觀察該病毒為典型的冠狀病毒;該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用,當繼代到第5代后,胚體嚴重病變;病毒在雞胚中隨著接種時間的延長,其效價增高, 96h可達到48h的2倍;該毒株可在cef上生長,但不能形成明顯的蝕斑;經1胰酶處理后可凝集雞紅細胞;雞胚的第四代尿囊液病毒回歸動物體,病死雞腎臟呈典型的花斑腎,腺胃則未見肉眼可見的病變。

The hemagglutinating activity of amphioxus skin mucus was decreased after heating at 60 for 30 min , and was almost lost upon heating at 100 for 30 min . the hemagglutinating activity of the skin mucus remained stable in the ph range from 6 to 10 , but it was inactivated at ph above 10 or below 6 . the hemagglutinating activity in the skin mucus is inhibited by d - galactose , which is same with the hemagglut inating activity of the humoral fluids of amphioxus 在60保溫30分鐘后,凝集素的凝集活性下降,在100保溫30分鐘后,凝集素凝集活性幾乎完全消失;在ph6 - 10的范圍內,凝集素的凝集活性穩定, ph大于10小于6的條件下,凝集素的凝集活性消失;文昌魚體表粘液中的凝集素受d -半乳糖苷的抑制。

The hemagglutinating activity of the fluids was not affected by treatment with 50 mm edta and egta , while it was increased by treatment with 3 - me 經注射雞大腸桿菌后,文昌魚體液中的凝集素對人b 、 o型紅血細胞,兔、草魚和蟾蜍紅血細胞的凝集活性明顯增強。

The hemagglutinating characteristic of jl94 was studied tentatively with human and 9 kinds of animal red blood cell ( rbc ) 采集人及9種動物的紅細胞,用病毒的細胞培養物做血凝和血凝抑制試驗,來檢測jl94的血凝特性。

The effects of metal ions and denaturants on the conformation and hemagglutinating activity of crotalaria mucronata lectin 金屬離子和變性條件對野花生豆凝集素活性和構象的影響

It is misspent time to calculate and report hemagglutinating titers for cell hybrid studies . 在細胞雜交過程中計算和報道血球凝集的滴定度是浪費時間。