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hapten n.【生、化】半抗原。haptenic adj.

haptene

The protein accounting for the total was approximately 11 . 80 % . the peptide was synthesized according to the sequence of grb - ast7 . since ast7 is a hapten , it was conjugated to the carrier proteins bovine serum albumin ( bsa ) using l - ethyl - 3 - ( dimethyl - aminopropyl ) carbodiimide ( edc ) 根據ast _ 7氨基酸序列合成小肽,用edc做偶聯劑把合成的半抗原小肽與載體bsa偶聯成完全抗原,免疫家兔三次后,采血收集抗血清,用elisa測定效價為1 400 。

A competitive binding assay used to determine hapten iaa with the aid of hrp - iaa conjugate has been developed . it was based on the ordered , stable mercapto self - assembled monolayer formed on gold surface , which immobilized igg with covalent bond 方法基于金基底上形成的均一、穩定、有序的巰基乙酸自組裝單層膜,在edc 、 nhs活化下,能以共價方式固定抗體。

The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation , the specificity of the antibody and the choice of a suitable enzyme label 酶免疫分析技術的質量依賴于抗原的純度、抗體的特異性、合適標記酶的選用,其靈敏度取決于標記酶的高度純化和高轉化率。