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germ layer 胚層。

germ theory

As a member of this superfamily , human acidic fibroblast growth factor ( afgf or fgf1 ) , expressed by a variety of cells from all three germ layers , shows great potential for clinical applications for therapy of a variety of diseases , such as parkinson ' s disease , spinal cord contusion injury , neural regeneration in reimplantation of broken - off finger , brain ischemia , renal ischemia , myocardial infarction , occlusive vascularitis , retinal ischemia , gastric ulcer and nonhealing wound and so on 人類酸性成纖維細胞生長因子( afgf , fgf - 1 )是成纖維細胞生長因子超家族成員。來自于三個胚層的多種細胞都可以表達afgf 。 afgf在治療帕金森綜合癥、急性脊柱扭曲性損傷、斷指中神經功能重建、腦缺血、腎缺血、心肌梗塞、閉塞性脈管炎、視網膜缺血、胃潰瘍及難愈合性傷口等多種臨床應用方面具有巨大潛力。

Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml , es - d3 cells being used in our experiments formed normal clones , expressed akp and kept their normal karyotype over many passages . the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers 結果顯示: eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因于億f )的es細胞培養液中形成典型的胚胎干細胞克隆,堿性磷酸酶染色結果為強陽性,具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能,而且具有形成種系嵌合動物的能力。

Embryonic stem ( es ) cell lines isolated from primitive embryonic cells are highly undifferentiated and totipotent cells . in vitro , es cells can spontaneously differentiate into embryoid bodies ( ebs ) containing derivatives of three germ layers 胚胎干細胞( embryonicstemcell , escell )系是從早期胚胎細胞分離培養而建立的高度未分化的具有全能性的細胞系。