galactoside n.【化學】半乳糖苷。
n. 【化學】半乳糖苷。 “alpha-galactoside“ 中文翻譯： α-半乳糖苷“beta galactoside“ 中文翻譯： β-半乳糖甙“galactoside acetylase“ 中文翻譯： 半乳糖苷乙酰化酶“galactoside acetyltransferase“ 中文翻譯： 半乳糖苷乙酰轉移酶“galactoside permease“ 中文翻譯： 半乳糖苷透酶“quercetin galactoside“ 中文翻譯： 槲皮素二葡萄糖甙,槲皮素半乳糖甙“quereetin galactoside“ 中文翻譯： 槲皮素半乳糖苷“beta-galactoside permease“ 中文翻譯： 半乳糖苷透性酶“cyanidin-3-galactoside“ 中文翻譯： 半乳糖甙“galactoside 3-fucosyltransferase“ 中文翻譯： 半乳糖苷3-巖藻糖基轉移酶“galactoside-binding protein“ 中文翻譯： 半乳糖苷結合蛋白“quercetin-3- galactoside“ 中文翻譯： 槲皮素-3-半乳糖甙,金絲桃甙,田基黃甙,海棠因“6-bromo-2-naphthyl-beta-galactoside“ 中文翻譯： 6-溴-2-萘酯-β-半乳糖苷“galactosidase deficiency“ 中文翻譯： 半乳糖苷酶缺乏癥“galactosidase“ 中文翻譯： 半乳糖激酶; 半乳糖醛酸; 半乳糖苷酶; 牛乳糖“galactosepermease“ 中文翻譯： 半乳糖透性酶“galactoseoxidase“ 中文翻譯： 半乳糖氧化酶“galactosemic fibroblasts“ 中文翻譯： 半乳糖血癥的成纖維細胞
galah |
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In this paper , first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv . the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector . after transforming e . coli dh5 a , ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr . presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc . comparing the aquired sequence of 3abc with that of reference strains , the homology is more than 99 percent . the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo . lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene , which happened to form a terminator codon behind 3ab gene , but it contained the complete open reading frame ( orf ) of 3ab gene . positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ) , bacteria were detected by sds - page and western blotting after properly treated . the results showed that the 3ab gene expressed successfully in e . coli and 33 . 5ku fusion protein can be recognized by the positive bovine serum of fmdv . the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis 擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段于3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，并能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 |
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Malvidin - 3 - o - galactoside chloride 氯化錦葵色素 |
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Isolation and preparation of potentilla anserina galactoside reference substance 蕨麻苷對照品的分離制備 |