F - fbs 中文

fbs FBS ＝ forward-based system 【...

fc
Subconfluent cultures of rat were maintained in dmem / 10 % fbs , 24hrs before neuronal induction , media were replaced with pre - induction media consisting of dmem / 10 % fbs / lmm beta - mercaptoethanol ( bme ) , to initiate neuronal differentiation , the preinduction media were removed , and the cells were washed with pbs and transferred to neuronal induction media composed of dmem / serum - free media , 5hrs later , 40ul dmso was given to every hole containing 2ml each 分別取第5代和第13代的mscs ，以8x10 cm ‘濃度接種于六孔板中的蓋玻片上，制備細胞爬片，每孔加zinl培養液。達到80融合時，更換新鮮培養液，并在培養液中加人終濃度為lrnm的p一流基乙醇，誘導24小時， pbs洗滌，而后換成無血清的培養液。
The main contents and results of the study are as follows : 1 . the separation , culture and purification of black bear fibroblast cell for the donor of nuclear transfer the black bear fibroblast cell can be obtained from ear skin and neck skin of the black bear by issue culture method . the cells were cultured in dmem and rpmi1640 mediums containing 10 % fetal bovine serum ( fbs ) , respectively 主要內容和結果如下： (一)黑熊成纖維細胞的分離培養和純化從黑熊耳緣皮膚和頸部皮膚取材，采用組織塊培養法分離培養黑熊皮膚成纖維細胞，在dmem和rpmi1640兩種培養基中分別添加10的胎牛血清，均可滿足黑熊皮膚成纖維細胞的體外生長和傳代。
The eg cell culture media consisting of dmem medium supplemented with fbs , chicken serum , beta - mercaptoethanol , l - glutamine . hepes , chicken embryonic extract and cytokines etc . after 24 hours culture , the isolated pgcs were selectively attached on the gonadal stromall cells in the plates 加入新鮮的eg細胞培養液(含dmem 、胎牛血清、雞血清、 -巰基乙醇、 l -谷氨酰胺、 hepes 、雞胚浸出液以及細胞因子等成分)培養24小時以后， pgcs開始部分貼附于共培養的生殖原基細胞上。
The enhancing factors of eb formation were studied . fifty thousand es - d3 cells per milliliter were incubated in dmem - high glucose supplemented with 20 % fbs and 2mm glutamine . the influencing factors which included hyclone fbs and homemade green season fbs , feeder cell layer , culture containers and 3 - mercaptoethanol ( - me ) were investigated 以5x10 ’加的eso3細胞種入含20胎牛血清和zinm谷氨酚胺的高糖dmem中，觀察進口胎牛血清和國產胎牛血清、飼養層、培養器皿以及卜硫基乙醇對es士3細胞形成的4debs數量的影響。
The main contents and achievements are articulated as follows : ( 1 ) problem solving in conceptual design in virtual enterprises the features and cause of ill structure of conceptual design problem have been analyzed . the representation of design state , including product specification and fbs framework , the methods and features of design decision , and problem solving based on knowledge in conceptual design stage are studied . based on the above work , it is pointed out that the cause of ill structure of design problem lies in the absence of knowledge for decision - making in the initial stage of design , and that capturing the knowledge for conceptual design decision is an effective method for reducing the degree of structural illness 本文就虛擬企業中概念設計問題求解、概念設計信息建模、概念設計知識的獲取以及概念設計決策知識的捕捉和集成這幾方面進行了深入系統的研究，主要研究內容和成果包括： ( 1 )虛擬企業基于知識的概念設計問題求解分析了概念設計問題病態結構的特點以及產生的原因，研究了概念設計狀態(產品規范和fbs框架)的表示、概念設計決策的方法和特點以及基于知識的概念設計問題求解，指出造成設計問題結構病態化的原因是在設計的早期階段設計決策知識的缺乏，提出捕捉概念設計決策知識是降低概念設計問題結構病態程度的有效方法。
In addition , we designed a test : the midgut was removed from spodoptera exigua larvae and was cut longitudinally into halves , the midgut epithelia were cleaned and digested with dispase ii , the pelleted cells in grace medium with 7 % fbs supplemented with 100units / ml penicillin , 100ug / ml streptomycin were cooled and then suspended in r18 - labelled virus 實驗結果表明，在幼蟲的病毒接種物中添加合適濃度的熒光增白劑( 0 . 01g . ml一’ )不會改變病毒在幼蟲中腸組織中的感染部位，卻可以提高幼蟲對病毒的敏感性，增加了病毒毒力。
In the fourth chapter , colored petri net ( cpn ) models of fbs are constructed , and through which the internal procedure of fbs and the relationship of mode and parameter status between different fbs are analyzed for preparation . in the fifth chapter , we discuss the development of function block application process 第四章介紹了一種有效的復雜系統分析模型? ?功能塊的著色petri網( cpn )模型，為后面的功能塊實現中的參數狀態傳播和模式切換部分做下技術準備。
This dissertation is trying to break through the boundaries of the traditional division by systematically investigating fbs , an issue of great practical and academic significance , from the angles of economics , sociology and biology 論文嘗試突破傳統學科分工的藩籬，從經濟學、社會學和生物學結合的視野，對這個具有強烈現實意義和重大學術價值的課題進行較為系統的專門研究。
Methods isolation , , culture and purification of mouse mscs mmscs were isolated from the femurs of adult mice four weeks old and propagated in vitro . mmscs were originally cultured in dmem - lg with 10 % fbs , 100u / ml penicillion , 100mg / ml streptomycin 在細胞沉淀中加人含10胎牛血清的dmem lg培養基，計數細胞數，調整細胞密度，按3x10vinl接種于25cm ‘的玻璃培養瓶中。
After being cultured three generations and depleted feeder cell layers , the es - d3 cells differentiated into ebs efficiently in the culture media supplemented with homemade green season fbs and - me in petriperm bacterial - culture dish 二、 inbmec cm誘導小鼠es細胞生成造血干祖細胞將獲得的4debs制成單細胞懸液，加入inbmec cm誘導4debs的單細胞生成造血干祖細胞。
Chapter 3 discusses entrepreneurs in fbs as the personal representatives . they progress in developing their human capital , physical capital , and social capital , which this thesis calls the three - dimensional development mechanisms of entrepreneurs 第三章，家族企業家作為家族企業人格化，其成長是基于人力資本、物質資本和社會資本三維機制的一個動態過程。
The clonies of es cells were flatter on the mitomycin c - inhibited sto fibroblasts , not easily differentiated from the feeder layer cells surround them , than that on the mef in dmem supplemented 15 % fbs . 5 以sto細胞作飼養層，添加15 fbs ，在倒置顯微鏡下觀察， es - d3細胞不易與周圍的sto飼養層細胞區別，形成的集落較平坦，生長狀態不如mef飼養層好。
Neuronal induction subconfluent cultures of mmscs were maintained in dmem - lg / 10 % fbs . 24h prior to neuronal induction , media were replaced with preinduction media consisting of dmem - lg / 20 % fbs / 1mm bme 2mmscs的神經誘導按以上方法制備細胞爬片，在神經誘導24小時前，培養基換成含有預誘導劑的培養基即合dmem ? lg 20 fbs lmmbme 。
Although modern economics has absorbed nutrients from other branches of social sciences and developed many new research areas , there are fewer literatures for analyzing the phenomena of fbs more comprehensively 雖然現代經濟學不斷吸收其它學科營養，拓寬新的研究領域，但至今對家族企業進行系統全面分析的文獻并不多見。
Why have most of the newly emerging enterprises during the economic transition in china been family businesses ( fbs ) ? why are fbs still the dominant form of economic organization in the world 為什么在轉軌期間中國新興的民營企業大都是家族企業，而且，在世界范圍內家族企業也普遍存在、持久不衰
The concentration of fbs ( fetal bovine serum ) and ncs ( newborn calf serum ) was influential on the culture process of mef ( mouse embryonic fibroblast ) and es cells 血清濃度的高低對胎鼠成纖維細胞和es細胞的培養過程是有影響的。無血清或低濃度的血清能抑制細胞分裂增殖，使細胞生長出現停滯。
In the later experiments dmem - lg / 20 % fbs / bfgf ( 50ng / ml ) was used as the neuronal preinduction media and dmem - lg / atra ( 150mg / ml ) was used as the neuronal induction media 在以后的實驗中， bfgf矚0n才rnl ）被用來作為前誘導劑，人ttiho50略inl ）被用來作為誘導劑。
The numhers of day - 4 ebs per milliliter culture media are 44 . 83 1 . 22 ( n = 6 ) and 43 . 17 1 . 05 ( n = 6 ) respectively when cultured by hyclone fbs and homemade green season fbs 在含進口hyclone胎牛血清或國產四季青胎牛血清的培養液中培養， es d3細胞形成的4debs數量分另為44
Through building functional , behavioral , and structural model , formal representation of function , behavior , and structure in fbs framework is realized 通過建立功能模型、行為模型和結構模型，實現了fbs框架中功能、行為和結構的形式化表示。

名稱
說明
信箱
電話
圖片	移動背景圖固定背景圖重製上傳圖片

fbs

fbs FBS ＝ forward-based system 【...

fc

Through building functional , behavioral , and structural model , formal representation of function , behavior , and structure in fbs framework is realized 通過建立功能模型、行為模型和結構模型，實現了fbs框架中功能、行為和結構的形式化表示。