E - explant 中文

explant vt.【醫學】外植，移植。n.外植體。n.-planta...

expletive
( 3 ) the optimal explant age for transformation was 6 or 7 day after aseptic germination of seeds . ( 4 ) development of non - transformated cell was inhibited completely by 100mg / l kan . ( 5 ) 500mg / l cb could avoid pollution of agrobacterium tumefaciens and had no distinct effects on the formation of callus and shoot regeneration . ( 6 ) the optimal co - cultural condition was 3 day on 28 in dark 以胡蘿卜品種“改良黑田”為受體材料，以胡蘿卜幼苗的下胚軸為外植體，建立了農桿菌介導法的胡蘿卜轉化體系，并對其中的種子消毒、激素水平、外植體的苗齡、選擇壓、共培養時間等關鍵步驟的條件進行了優化。
The process of initial culture showed quite difference in various hostas . h . ventricosa and h . “ so sweet “ were easier , while h . “ frances williams “ and h . “ gold standard “ were difficult . using shoot tip ( peak bud ) as explant was proved the effective method for improving survival ratio when hostas would defoliate or have defoliated in autumn or winter rather than in summer 玉簪屬不同種類的組培初代建立存在著一定的差異，如h . ventricosa和h . ‘ sosweet ’較容易建立，而h . ‘ franceswilliams ’和h . ‘ goldstandard ’比較困難；應在冬季玉簪屬植物將要落葉或已落葉后取莖尖(頂芽)進行初代建立，最好避開夏季，來提高初代建立的成活率。
The materials as explant in transformation come from birch leaf , stem segment and leaf stalk , and the spider toxin gene was used as foreign gene for this transformation experiment . it showed that the best explant was the big leaf , on which the transformation frequency was 22 % . by gus detection , there were 43 percent of the plants with kanamycin resistance , and 100 percent of positive result , by pcr amplification , was gotten from random sampling 利用雙元載體的根癌農桿菌lba4404菌株( agrobacteriumtumefaciens ) ，含質粒pyhy (目的基因及npt 、 gus基因) ，對白樺試管苗莖段，葉柄，葉片三種外植體進行侵染，結果表明：大葉片生長勢強，為轉基因的最優外植體，轉化率能夠達到22 。
In this paper , three plant expression vectors concerning chilling tolerance were constructed . lba4404 ( pbin + - rd29a - gus + pal4404 ) was used to transform explant materials of cucumber and micro - tomato , and the transient expression of gus gene was detected , whether chilling inducing expression trait of dehydration and low - temperature inducing promoter p - rd29a are charactered in it was primarily discussed 通過將攜有rd29a - gus - tnos基因表達盒的載體pbin ~ + - rd29a - gus轉化黃瓜和小番茄外植體材料，檢測gus基因的瞬間表達活性，初步探討了冷誘導啟動子p - rd29a在這兩種作物中是否具有冷誘導活性的問題。
With 24 c or 4 c , the change trends of the content of the salidroside was basically consentaneous in the consecutive cultural eras of the callus . in the callus from the different explants , the influence was maximal to the the activity of pal enzyme and the influence was lowest to the the activity of ca4h enzyme , and the influence to the the activity of tal enzyme was ascertained according to the explant of the callus . so in the same explant with the different temperature or in the different explant with the same temperature , there was no incident between the content of the salidroside and the activity of enzymes pal , ca4h and tal , and we presumed that there may be emphasized particularly on different metabolic pathway of salidroside ( 2 ) 、愈傷組織的外植體來源、培養溫度條件和不同的繼代培養數都影響著其中紅景天甙的含量和苯丙氨酸解氨酶( pal ) 、肉桂酸解氨酶( ca4h )和酪氨酸解氨酶( tal )這3種酶的活性；不管是葉來源還是莖來源，不管是24培養還是4培養的愈傷組織，在連續繼代培養中紅景天甙含量的變化趨勢基本上是一致的；無論是葉來源的愈傷組織還是莖來源的愈傷組織，培養溫度對pal酶的酶活性影響最大，對ca4h酶的酶活性影響最小，對tal酶的酶活性影響視不同的外植體來源而定；在相同外植體來源的愈傷組織中及不同的培養溫度的條件下，或是在不同外植體來源的愈傷組織中及相同溫度的培養條件下，其紅景天甙含量與pal酶、 ca4h酶和tal酶的酶活性之間沒有完全一致的對應伴隨關系。
( 1 ) plant regeneration system of cucumber contyledonary node was established . ( 1 ) the cotyledonary node was regarded as optimal explant by comparing the differentiation frequency of 3 kinds of explants ( cotyledon , cotyledonary node and hypocotyl ) . ( 2 ) by discussing the effect of pgr ( naa and 6 - ba ) concentration and combination on differentiation frequency of adventitious buds , we concluded that the optimal shoot induction medium is ms basal medium with 1mg / l 6 - ba ( ph5 . 8 ) ( 1 )黃瓜子葉節植株再生建立東北農業大學理學碩士學位論文一通過對比三種外植體（于葉、子葉節、下胚軸）的分化率，得出最佳外植體類型為子葉節；通過探討pgr （ naa 、 6 ba ）濃度和配比對不定芽分化的影響，得出最佳芽誘導培養基為：卜1叭6 。
Cotyledon without petiole showed a significantly lower frequency of shoot regeneration ( 16 . 7 % ) compared with that of entire cotyledon ( 33 . 8 % ) , but the average number of shoots per explant ( 4 . 2 ) was more than that of entire cotyledon ( 2 . 5 ) . no shoot was obtained when the petiole alone was cultured . the frequency of shoot regeneration ( 8 . 3 % , 12 . 5 % ) and average number of shoots per explant ( 2 . 3 , 1 . 7 ) from the upper section of lamina or one side of lamina was lower than those of entire cotyledon 另一方面，以“種都一號”兒菜的不同子葉部位作為外植體所進行的研究結果表明，不帶子葉柄的完整葉片其芽再生率( 16 . 70 )顯著低于帶子葉柄的完整子葉( 33 . 8 ) ，但平均再生芽數，前者( 4 . 2 )卻高于后者( 2 . 5 ) ；子葉柄不能進行芽的分化；不帶子葉柄的子葉尖端和子葉一側，其芽再生率(分別為12 . 5 、 8 . 30 )及再生芽數(分別為2 . 3 、 1 . 7 )也明顯低于帶子葉柄的完整子葉。
Carrot tissue culture and plant regeneration factors including explants , medium and culture condition are combined together to study the most efficient protocol of carrot tissue culture and plant regeneration thereof . the most suitable explant is fresh hypocotyls segment and precultured hypocotyls derived from 7 - 10 day old aseptic plantlets generating in dark or in dim light , the best recipe for cullus induction and subculture is b5c ( 85 with 0 . 5mg / l 6ba and 0 . 5mg / l 2 , 4 - d ) , the ideal recipe for plant regeneration is 65 or ms free of hormone . a phytotron with a 16 / 8 h day / night cycle , at 25 is feasible for plant regeneration , and occasional exposure to sun light dramatically stimulates plant growth 建立了高效的胡蘿卜組織培養及再生體系以適于生產飲料的胡蘿卜“新黑田五寸人參”為材料，研究不同外植體、不同培養基，不同培養條件對胡蘿卜愈傷誘導及再生的影響，建立一套高效的胡蘿卜組織培養再生體系：最適于誘導愈傷的外植體是弱光或黑暗下發芽7 - 10d無菌苗下胚軸，最適合的愈傷誘導培養基和繼代培養是b _ 5c ( b _ 5 + 0 . 5mg l6ba + 0 . 5mg l2 ， 4 - d ) ，最適于植株再生的培養基為不添加任何激素的b _ 5或ms ，組織培養條件為25 、光照周期為16hr 8hr 。
4 . after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time , incubation time and co - culture time , we set up the system of high frequency transformation of tomato cotyledons . then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation , and the resistant plants to hyg were obtained . by pcr analysis on part of the putative transformants , we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants . 5 . transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg . trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection , growth and bio - morphology analysis , hyg - resistant experiment and pcr analysis 通過pcr檢測證實部分番茄抗性植株中已導入hbmp - 3m基因；人骨形成蛋白一3成熟膚基因和全長基因分別轉化番茄和煙草的研究5 .通過農桿菌介導法將hbmp一3全長基因導入煙草，并且獲得了hyg抗性植株，通過gus基因瞬時表達檢測、轉化植株的生長情況及形態學分析、 hyg抗性鑒定及pc尺檢測，證明目的基因己經整合到煙草基因組中。
S . regeneration system of mustard hypocotyls and cotyledons of 4 days and leaves of 25 days were used as transformation materials , and ms as basic medium supplying different ba ^ naa and kt concentration , we got the optimum explant adventitious bud inducing medium ( ms + 6 - ba3 . 0mg / l + naa0 . 2 mg / l ) . among the three kinds of mustard explants . the cotyledons showed the highest adventitious bud regeneration capacity . and the regeneration capacity of leaf explantswas weaker . and the weakest of hypocotyls 芥菜高頻再生體系的建立以4天左右的葉用和莖用芥菜子葉、下胚軸和25天苗齡的芥菜無菌苗的葉片為外植體，以ms為基本培養基，通過加入不同濃度和不同組合的ba 、 naa 、 kt ，篩選到了外植體不定芽分化的最適培養基( ms + ba3 . 0mg / l + naa0 . 1mg / l )及生根的最適培養基( ms + naa0 . 2mg / l ) 。
The genotype is a main factor in the genetic transformation via agrobacteriwn - mediated . the results of orthogonal experiments of the factors which affect mainly the transformation illuminates that the erniuxin genotype + bacterium 15 x + explant + dip - dye is the best one . the cocultivation time is 28 days . the experiment shows that : the erniuxin cotyledon regeneration frequency is very high , and that the dongnong901 hypocotyl regeneration frequency is very high 確立了農桿菌介導轉化過程中基因型是影響轉化率的主要因素。通過極差分析，基因型為“二牛心” ，菌液濃度15倍的，外植體為子葉，感染方法為浸泡法是最佳的組合。農桿菌與外植體共培養的時間為28h 。
The callus was induced and proliferated using young leave as explant , which comes out at early spring . in this paper , the author discussed the induction effect and the proliferation effect by using different kinds of hormone and different physical - chemical factors 本文以山茶初春新生枝條的幼葉為外植體，采用不同的培養基，探討了不同的植物生長調節物質對愈傷組織的誘導和不同的理化因子對愈傷組織的增殖效應。
In this experiment , the immature embryos were used as explant to induce calli and bt gene was tranfered into mo17 , 81162 , ji 846 and 7922 by the agrobactrium - mediated . we have gained the positive plants determined by pcr 本論文以東北地區骨干玉米自交系幼胚為外植體，采用農桿菌介導法將bt基因導入了mo17 、吉846 、 81162及7922 ，并獲得了pcr檢測呈陽性的再生植株。
We confirmed the correct construction by pcr and restriction enzyme analysis . in this research , hypocotyls were used as the explant and several factors affecting genetic transformation of carrot mediated by agrobacterium tumefaciens were studied 利用vp7基因和質粒pbi121上相同的單克隆位點，將vp7基因定向克隆到植物表達載體pbi121上，構建了pbi121vp7表達載體。
Research also indicate that explant influence the frequency of callus induction , especially influence embryogenic calli formation . 5 . the proper concentration of ppt is 3 . 0mg / l in screening resistant callus after transformation 在轉化后抗性愈傷組織篩選中，適應的ppt篩選濃度為3刀mg幾， cef有效抑菌濃度為200pg ml ，乙酚丁香酮有效誘導濃度為100pmol幾， 0
To answer when the retinal explant of rat loses the neurite regeneration in vitro after birth , we have used tissue culture to study the neurite regeneration of retinal explant of wistar rat at different ages 為了解答大白鼠的視網膜人工培養在出生后多久就會喪失軸突再生的能力，我們利用出生后不同年齡的大白鼠之視網膜進行組織培養。
The development of study on tissue culture of orchid is summarized . the program of issue culture , the choice of explant and medium , the ways and condition of culture are introduced 摘要對蘭花的組織培養進行了綜述。著重闡述了蘭花組織培養中組織培養程序、外植體的選擇、培養基與激素的選擇、培養方式和培養條件的研究進展情況。
Addition of img / l tdz and img / l naa resulted in the highest bud regeneration rate ( 67 . 9 % ) and number ( 35 ) of buds per explant . regeneration rate could be improved apparently in the medium with agno3 1mg ltdz和1mg lnaa配合對子葉再生具有極強的誘導作用，再生率最高，達到67 . 9 ，平均每外植體不定芽數達35個。
However , when immature embryo was used as explant , the optimize concentration of 2 , 4 - d is different , as for yangmai 87158 and ann 98005 , it is 2 . 0 mg / l , but as for ann92484 , it is 4 . 0mg / l Omg l時出愈率和胚性愈傷組織誘導率達到最大值，而對于幼胚不同品種表現有所差異，其中楊麥87158和安農98005在2 ， 4濃度為2

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explant

explant vt.【醫學】外植，移植。n.外植體。n.-planta...

expletive