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enucleate vt.1.〔古語〕闡明。2.【醫學】摘出,剜出。3.【生...

enumerable

These observations show that reprogramming is easier in interspecific embryos reconstructed with es cells than that reconstructed with somatic cells , and that es cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells . oocytes were reconstructed with outbreeding kunming albino mouse es cells and enucleated rabbit oocytes , and the effects of the passages of es cells and 6 - dmap on the development of interspecific reconstructed oocytes were analyzed . the interspecific reconstructed es - rabbit oocytes were activated either by combined two set electric pulses and 6 - dmap or by two set electric pulses 以上結果顯示, 6 dmap能增加胚胎干細胞異種重構卵的卵裂率,對重構卵囊胚的發育率影響不大;高培養代數和低培養代數的胚胎干細胞用于異種核移植不影響異種重構卵的卵裂率和囊胚發育率;用胚胎干細胞作為供核細胞;比體細胞作為供核細胞所構建的異種重構胚更容易進行重編程,并且胚胎于細胞指導異種克隆胚胎正常發育的能力強于體細胞。

The paper gave out that these problems were resulted in the challenge of conflict of individual value and community value , the shock of value pluralist trend , the influence of diversity of value idealism and customs , the improper deal with the contradiction of social fair principle and social utility , and unbalance of development caused by region variety , also the paper explains that labor - technology education is the important way to promote the junior middle school students “ allover development by enucleating that labor - technology education has the functions such as grooming virtues , enlightening wisdom , benefiting goodness , strengthening mind and body , accelerating creation and exercising living and practice ability 本文討論了初中階段勞動技術教育的內涵及其結構,分析了當代初中勞動技術教育存在著的諸如制度欠缺、教材陳舊、方法簡單、投入不足、師資不穩、評估薄弱等一系列問題,并認為產生這些問題的根源在于:個體價值與群體價值沖突帶來的挑戰、價值多元化趨勢的沖擊、價值目標的理想性與世俗性對立造成的影響、社會公正原則與社會功利矛盾的處理不當以及地域差異導致的發展失衡。

Recovery of microtubule organizing centers in enucleated oocytes and condensation of chromosome and organization of spindle in reconstructed embryos were analysed . a non - invasive method of enucleation mouse oocytes has been developed . firstly , the progression of mouse oocytes in meitosis i was ascertained by immunofluoresence technique 另外,本試驗通過在m16基礎上添加eaa 、 non - eaa和葡萄糖顯著提高了胚胎的桑椹率、囊胚率和孵化率;單獨添加葡萄糖的培養液,其桑椹率顯著升高,但囊胚率和孵化率的升高并不顯著,所以胚胎培養液中氨基酸和葡萄糖能協同促進胚胎發育。

Like the oocytes of aged mice , the reconstituted oocytes by gvs of aged mice and enucleated gv oocytes of young mice showed significantly increased frequency of premature chromatid separation , spindle disruption and / or chromosome misalignment . in contrast , the reconstituted oocytes by gvs of young mice and enucleated gv oocytes of aged mice behaved 由不同校質比gv期卵母細胞成熟后的mll期卵母細胞以及發育成的原核期胚和2一細胞期胚中pkcq的分布并不受細胞質減少的影響。

By nuclear transferee transfered the nih 3t3 cell to enucleated gv and mii oocyte . the immunofluorescence results showed that jak . 2 was not transfered to somatoblast nuclear whether the recombined oocyte occured gvbd or not . when transfered somatoblast nuclear to enucleated and integrated m ii oocyte , the immunofluorescence results showed that jak2 only existed in the nuclear of integrated m ii oocyte but not the somatoblast nuclear . the nuclear transfer implied that the mature and immature oocyte cytoplasm could not transfer the jak . 2 protein to donor nuclear 通過核移植,將nih3t3體細胞核移入去核gv期和m期的卵母細胞中。熒光檢測發現,體細胞核與去核gv期卵母細胞質構成的重組卵無論是否發生gvbd ,核內均沒有jak2的出現。在去核和未去核的m期卵母細胞中移入體細胞核, jak2僅存在于未去核m期卵母細胞的核內,并不轉移到體細胞核內。

This simple two - step chemical enucleation procedure yields fully enucleated mouse oocytes in 85 . 6 % of cumulus - oocytes complexes and 56 % of natural oocytes , significantly different ( p < 0 . 05 ) . since the enucleation rate of cumulus - oodles complexes is equivalent to that by micromanipulation , induced enucleation will greatly improve the whole efficiency in mouse somatic nulear transfer 該方法完全丟棄了傳統核移植的顯微操作及其繁瑣程序,是純粹的手工克隆( hmc ) ,它的成功將會大大簡化核移植程序,提高核移植效率,加快其產業化的步伐。

The enucleated rates can reach at 87 . 06 % . 3 ) three activated methods may effectively activate the black bear - cat reconstructed embryos . whereas activated effect of ethanol + 6 - dmap + ccb is more effective , the cleavage rate and the development rate to m 重組胚在3種培養液中的發育能力有差異t 0刀引, m199成熟培養液比dmem12 、 m16更能支待重組胚胎的卵裂和桑棋胚發育,卵裂率和桑堪胚發育率分別為63

It mainly introduced some basic conceptions such as the maritime search and rescue . and it also enucleated the research sense of the new search and rescue system on the changjiang river . in this part , the dissertation ascertained the dissertation ' s technical route 主要介紹水上搜救的基本慨念和水上救助的基本形式,闡明長江水上搜救的目的和重要意義,明確論文的研究方向和總體思路。

It is even more interesting that the activation of some pathways , i . e . , the activation of camp - pka - creb and clustering of cellular surface receptor egfr seem to be independent of dna damage , because these events can still occur in enucleated cells 更值得注意的是這些有關信號轉導通路的激活有的并不依賴于細胞核中的dna損傷,因為在除去細胞核的細胞中有些細胞通路的激活仍可被誘發。

Theory of self - calibration of platform was enucleated . process of self - calibration of platform discussed in this thesis was given . observability of multi - position error calibration was studied , then calibrate the errors of the platform 闡明平臺誤差自標定原理,提出本文研究的平臺系統自標定過程,并研究多位置翻滾試驗下的可觀測性分析,進行平臺誤差系數的標定。

It is even more interesting that the activation of these pathways seems to be independent of dna damage , because these events can still occur in enucleated cells 更值得注意的是這些有關信號轉導通路的激活并不依賴于細胞核中的dna損傷,因為在除細胞核的細胞中這些細胞通路的激活仍可被誘發。

We also sought to determine whether we could induce human eggs to divide into early embryos without being fertilized by a sperm or being enucleated and injected with a donor cell 我們也想知道,卵子是否可以無需精子的受精作用,或不必去核再注入其他細胞核,就可以直接分裂成早期胚胎。

No pregnant rats had been observed for 20d . this event indicated mouse enucleated maturation oocytes ca n ' t support the donors of rat fibroblast cells to develop a viable offspring 將255枚2細胞以上的核移植重構肚,移植給46只sd假孕鼠,均未妊娠。

In this study , we found that enucleated maturation oocytes of bovine and mouse can only support nuclear transfer embryos to develop to morula 60o和8 50o的桑堪胚發育;重組胚胎在mi99和改良的czb中培養,分別獲得16 41o和17

This paper discussed purposes , functions and management of software engineering , enucleated principles it should follow 本論文探討了軟件工程的目的、作用及管理方法,闡明了軟件工程應遵循的原則。

4 ) the enucleated rate ( 88 . 23 % ) of the mouse maturation oocytes were improved in the medium containing 10 ? ug / ml ccb 研究發現10土5ug ml的ccb有利于去核(去核率為88

Enucleated cells are prepared by removing cell nuclei in the presence of the drug , cytochalasin b 無核細胞是通過藥物、細胞松馳素b把細胞核去掉制備的。

After another 8 - year period follow - up , two small recurrences were noted and were easily enucleated 因其再發率特高,長期的追蹤是必須的,故提此報告。

To a mother ' s enucleated egg 母親的無細胞核卵子